Fish species accumulation curve for Madagascar Reef. Calculated using sample-based incidence data with the software EstimateS v.9 (http://purl.oclc.org/estimates) using Eq. 5 in Colwell et al. (2004) and Eq. 17 in Colwell et al. (2012) for rarefaction and Eq. 18 in Colwell et al. 2012Colwell et al. (2012) for extrapolation. Bars represent 95% confidence intervals calculated using Eq. 6 in Colwell et al. (2004) for rarefaction and Eq. 19 in Colwell et al. (2012) for extrapolation. For a full output of the analysis see Suppl. material 3.