Natural history of Astrotischeria karsholti Puplesis & Diškus, 2003 in the Azapa Valley, Arica Province of northern Chile. A The host plant Ambrosia cumanensis Kunth (Asteraceae) in the neighbourhood of a citrus orchard; B, C Leaf mine on A. cumanensis previous to nidus formation, adaxial and abaxial views, respectively; D, E Leaf mine at the beginning and at the end of nidus formation, respectively, abaxial view; F Last instar larva removed from mine of B and C; G Nidus of D and E opened to show the pupa.