Neighbour-joining tree of Southern Appalachian Pseudoscorpiones barcoding sequences, based on K2P distances. Vertical boxes on the right of the tree indicated ASAP-inferred species level lineages, based on 3, 5 and 8% distance thresholds (yellow boxes: populations exclusively west of the Asheville Depression; blue boxes: populations to the east; green boxes: populations on both sides). Black stars indicate specimens illustrated on the right.