Phylogeographic analysis of Leuctra prima and Leuctra sp. ZB, based on the COI gene fragment, 658 bp in length. A – ML phylogenetic tree. The terminals correspond to the BOLD IDs. Individuals collected in present study are marked with an asterisk and Leuctra sp. ZB specimens with triangles. Bars in different colours represent the results of species delimitation methods. B – Phylogenetic network obtained by the statistical parsimony (TCS network). Colour coding corresponds to the groups in the species tree. C – Geographical distribution of analysed haplotypes. Colour coding corresponds to the groups in the species tree and the TCS network.