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Biodiversity Data Journal 14: e173579
https://doi.org/10.3897/BDJ.14.e173579 (08 Jan 2026)
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  • Introduction
  • Materials and Methods
    • Sample collection and RNA extraction
    • Illumina sequencing and de novo assembly
    • Functional annotation
    • Development of EST-SSR markers
    • DNA extraction and microsatellite genotyping
    • Genetic diversity analyses
    • Population structure and phylogenetic inference
  • Results
    • Illumina sequencing and de novo assembly
    • Functional annotation and classification of unigenes
    • Frequency and distribution of SSRs in the unigenes
    • Genetic diversity
    • Genetic structure and cluster analysis
  • Discussion
    • Characterization of Transcriptome
    • Frequency and distribution of EST-SSRs
    • Genetic diversity and population structure
    • Conservation implications
  • Conclusions
  • Acknowledgements
  • References
  • Supplementary files
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