First record of predation of Nyctinomops laticaudatus (É. Geoffroy, 1805) by Chrotopterus auritus (Peters, 1856) (Mammalia: Chiroptera)

Abstract The Wooly False Vampire Bat, Chrotopterus auritus (Peters, 1856) (Chiroptera: Phyllostomidae), feeds on small mammals, birds, lizards, frogs and occasionally large insects and fruits. In this paper we report an additional evidence of bat predation by C. auritus. A male of this species was captured with a partially eaten Broad-eared Free-tailed Bat, Nyctinomops laticaudatus (É. Geoffroy, 1805) (Chiroptera: Molossidae). This record was obtained during a research project conducted in the Biological Reserve of Sooretama, Southeastern Brazil.


Introduction
Neotropical bats have the highest diversity of dietary habits among mammals, including nectar, fruits, blood, insects and vertebrates (Kunz et al. 2011). Several Phyllostominae (Phyllostomidae) species occasionally feed on small vertebrates, but the prevalence of regular carnivory is unique to the tribe Vampyrini (Baker et al. 1989;Bonato et al. 2004;Giannini and Kalko 2005;Simmons 2005). This correlation between phylogeny and feeding habits reinforces the importance of dietary studies within Phyllostomidae.
Chrotopterus auritus is a large Vampyrini that feeds on small mammals, birds, frogs, reptiles, insects, and occasionally fruits (Peracchi and Albuquerque 1976;Medellin 1988;Giannini and Kalko 2005). In Phyllostomidae and Noctilionidae, the consumption of small vertebrates is associated with increased body size (Peracchi and Albuquerque 1976;Wetterer et al. 2000). The species C. auritus may present opportunistic feeding habits (Wolda 1988;Bonato et al. 2004). However, according to Bonato et al. (2004), small mammals represent more than 70% of the biomass consumed by that species, and may include bats. Nevertheless, cases of consumption of bats by C. auritus are very rare (Nogueira et al. 2006).

Methods
This research was conducted in the Biological Reserve of Sooretama, a protected area of 24,250 hectares formed by a large area of primary Atlantic Forest, located mainly in the municipality of Sooretama but also spanning to Jaguaré, Linhares and Vila Valério in northern Espírito Santo, Southeastern Brazil. The climate is Tropical (Am in Koppën's classification), with a rainy season in the summer and a short dry season in the winter. Average temperature is 23 ºC, and annual rainfall is 1250.5 mm. The dominant vegetation is lowland dense tropical rainforest (MMA 2007).
Capture of the bats was performed using mist nets placed at ground level, on a trail in the far eastern region of the conservation unit (19°02'44"S and 39°57'30"W, elevation of 38 m). An adult male of C. auritus was captured in December 2011. Simultaneously, 15 cm below it, the remains of a molossid bat, the lower body intact, were recovered (Fig. 1). The voucher specimen of C. auritus was placed in 70% ethonol for long-term preservation and deposited at "Laboratório de Estudo em Quirópteros" (LABEQ), Universidade Federal do Espírito Santo (UFES), Brazil, under the code VP450. The individual of C. auritus had feces and intestinal contents collected and its components were analyzed using a stereoscopic microscope. The fragments that could represent parts of bats were separated to assist in identifying the prey.

Results
In the intestinal contents, an intact tooth was found, and we could identify it as a premolar (P3) of Nyctinomops laticaudatus (Fig. 2) using identification keys (Gregorin and Taddei 2002;Gregorin and Cirranello 2015). The specimen has a dark brown dorsal coat, with lighter brown hair with whitish tips on the ventral region. The hair around the toes are almost twice as long as the toes themselves (Fig. 1). Measurements of external structures were taken for comparison with other species of this family occurring in the region. A specimen of Nyctinimops macrotis, found in the same locality by Hoppe et al. (2014), was included in this comparative analysis (Table 1). No fragments of mist net were found in the intestinal tract, feces, or even in the mouth of the specimen of C. auritus, and the net around the body of the molossid bat was intact.
It is important to emphasize that this record does not provide information about the time and place where predation occurred. Thus, there are two possible scenarios: 1) the predation of N. laticaudatus occurred while this individual was in the mist net; 2) the predation occurred elsewhere and therefore C. auritus was captured with its prey.
Complementary studies are needed in order to know more details regarding the diet and feeding behavior of C. auritus.