1urn:lsid:arphahub.com:pub:F9B2E808-C883-5F47-B276-6D62129E4FF4urn:lsid:zoobank.org:pub:245B00E9-BFE5-4B4F-B76E-15C30BA74C02Biodiversity Data JournalBDJ1314-28361314-2828Pensoft Publishers10.3897/BDJ.8.e510925109213340Taxonomic PaperColeopteraCoccinellidaeTaxonomyChinaAsiaNew records and checklist of Chilocorini (Coleoptera: Coccinellidae) from ChinaLiWenjinghttps://orcid.org/0000-0002-3365-12191ChenBingxu1HuoLizhi2ChenXiaoshenghttps://orcid.org/0000-0001-8253-49432WangXingmin32457430@qq.com2Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaGuangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural SciencesGuangzhouChinaKey Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, Guangdong Province; Engineering Research Center of Biological Control, Ministry of Education & Guangdong Province, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, Guangdong Province; Engineering Research Center of Biological Control, Ministry of Education & Guangdong Province, South China Agricultural UniversityGuangzhouChina
Corresponding author: Xingmin Wang (32457430@qq.com).
Academic editor: Yasen Mutafchiev
2020240620208e51092AD6F8FCD-4AE8-5006-8DBA-551EEB676FB739269561302202010062020Wenjing Li, Bingxu Chen, Lizhi Huo, Xiaosheng Chen, Xingmin WangThis is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Background
China is one of the countries with the greatest species diversity of Chilocorini (Coleoptera: Coccinellidae), including nearly forty-five percent of the known genera and fourteen percent of all described species in this tribe. Recently, we discovered three species previously not recorded in China.
New information
In this study, three species Priscibrumusuropygialis (Mulsant, 1853), Priscibrumusdisjunctus Canepari, 1997 and Brumusoctosignatus (Gebler, 1830) are documented for the first time in China. Brumusoctosignatus is the first member of the genus Brumus Mulsant, 1850 recorded in China. Detailed descriptions, illustrations and distributions of these three species are provided. A checklist of Chinese Chilocorini is also given.
The members of family Coccinellidae, commonly known as colourful and shiny beetles, predators of plant pests, contain 6000 species distributed worldwide (Vandenberg 2002). The tribe Chilocorini is well-known as a primary predator of coccids. Many species of this tribe are widely used as biological control agents. In recent years, phylogenetic and evolutionary studies of Chilocorini indicated that this tribe is a monophyletic group, closely related to the tribe Coccinellini (Magro et al. 2010, Seago et al. 2011, Escalona et al. 2017, Che et al. 2017) or Plotinini (Li et al. 2020). At present, Chilocorini contains 22 genera and more than 280 species distributed worldwide (Łączyński and Tomaszewska 2012, Li et al. 2020).
China is one of the countries with the greatest species of Chilocorini (Coleoptera: Coccinellidae), including 10 genera and 39 species (Li et al. 2017a). However, in Li et al. (2017a), the genus Brumus Mulsant was incorrectly recorded in the Chinese checklist of Chilocorini (no literature existed to indicate that the members of this genus were distributed in China). After a phylogenetic study of the Chilocorini, the genus Phaenochilus Weise was synonymised with Chilocorus Leach (Li et al. 2020).
In this study, we report for the first time in China the genus Brumus with the species Brumusoctosignatus (Gebler, 1830), as well as the species Priscibrumusuropygialis (Mulsant, 1853) and Priscibrumusdisjunctus Canepari, 1997. A revised checklist of Chinese Chilocorini is also provided, containing all nine genera and 42 species.
Materials and methods
Specimens, examined in this study, were collected in China (Tibet and Xinjiang) and deposited at the Department of Entomology, South China Agriculture University (SCAU), Guangzhou.
The newly-collected specimens of Priscibrumusdisjunctus were identified based on the original species description (Canepari 1997). The specimens of the other two new Chinese records i.e. Priscibrumusuropygialis and Brumusoctosignatus, were identified from the secondary descriptions and illustrations by Miyatake (1985) and Kovář (1997), respectively.
External morphology was observed with a dissecting stereomicroscope (SteREO Discovery V20, Zeiss). Male and female genitalia were dissected, cleared in 10% solution of sodium hydroxide (NaOH) by boiling for several minutes and examined with an Olympus BX51 microscope. Photographs of the genitalia and other morphological characters were taken with digital cameras (AxioCam HRc and Coolsnap-Procf & CRI Micro*Color), attached to microscopes using AxioVision Rel. ver. 4.8 and Image-Pro Plus ver. 6.0. Images were cleaned up and laid out in plates with Adobe Photoshop CS ver. 8.0. Terminology follows Ślipiński (2007).
Abbreviations
TL = total length: length from apical margin of clypeus to apex of elytra
TW = total width: width across both elytra at widest part
TH = body height measured across the highest point of the elytra
HW = head width in a frontal view
PL = pronotal length: from middle of anterior margin to base of pronotum
PW = pronotal width at widest part
EL = elytral length: from apex to base including scutellum
Priscibrumus can be distinguished from other genera of the tribe Chilocorini by the following combination of characters: body densely covered with short, greyish pubescence; antenna composed of 10 antennomeres, with terminal antennomere very small and embedded in the penultimate antennomere; pronotal basal margin entirely bordered with submarginal line; base of pronotum and elytra contiguous all along their length; elytral epipleura narrow, more or less horizontal and without foveae; abdominal postcoxal lines almost complete; mid and hind tibiae with two apical spurs.
DBFEBD22-DF86-5F7D-9C90-20724315E3A3PriscibrumusdisjunctusCanepari, 1997Priscibrumusdisjunctus Canepari, 1997 in Canepari 1997: 45.Materials
TL: 3.75–4.10 mm, TW: 2.80–2.91 mm, TH: 1.83–2.12 mm, TL/TW: 1.31–1.34, PL/PW: 0.45–0.47, EL/EW: 1.07–1.10.
Body oval, moderately convex. Head black, densely covered with short, greyish pubescence. Mouthparts and antenna black. Pronotum black, densely covered with short, greyish pubescence. Scutellum black. Elytra reddish-brown, with two pairs of broadly black stripes: outer stripes approximately 2/5 width of elytra, 3/4 length of elytra; inner stripes situated on suture, almost as long as elytra, distinctly broadening at base and weakly broadening at apex, densely covered with extremely short, greyish pubescence (Fig. 1a–c). Underside black except elytral epipleura brownish-yellow, densely covered with short, greyish pubescence. Abdominal postcoxal lines incomplete, arcuate. Posterior margin of male abdominal ventrite 5 slightly emarginate at middle and of ventrite 6 distinctly emarginate (Fig. 1d).
Male genitalia: penis slender, penis capsule with short outer and inner arm, apex of penis truncate with membranous appendage (Fig. 1e–f). Tegmen stout, penis guide narrow at base, parallel along basal 1/3, after that, gradually broadening to basal 2/3, then narrowing to apex in ventral view; penis guide in lateral view, widest near base, gradually narrowing to apex; parameres distinctly longer than penis guide with dense short setae on the inner sides and distal end with a group of short setae in lateral view (Fig. 1g–h).
Female genitalia: coxites distinctly elongated. Spermatheca approximately C-shaped, cornu without appendage.
Diagnosis
This species can be distinguished from other species of Priscibrumus by the following combination of characters: elytra reddish-brown, with two pair of broadly black stripes, inner stripes situated on suture, almost as long as elytra; parameres distinctly longer than penis guide.
Distribution
Nepal (Canepari 1997) and Tibet, China (present study) (Fig. 4).
TL: 3.65–4.24 mm, TW: 2.72–3.31 mm, TH: 1.46–1.82 mm, TL/TW: 1.28–1.34, PL/PW: 0.46–0.51, EL/EW: 1.07–1.16.
Body oval, moderately convex. Head black, densely covered with short, greyish pubescence. Mouthparts and antenna black. Pronotum black, densely covered with short, greyish pubescence. Scutellum black. Elytra reddish-brown, with a pair of black spots at elytral apex, densely covered with short, greyish pubescence (Fig. 2a–c). Underside black, except elytral epipleura brownish-yellow, densely covered with short, greyish pubescence. Abdominal postcoxal lines incomplete, arcuate. Posterior margin of male abdominal ventrite 5 slightly emarginate at middle and of ventrite 6 distinctly emarginate (Fig. 2d).
Male genitalia: penis slender, penis capsule with short outer arm and long inner arm, apex of penis truncate with membranous appendage (Fig. 2e–f). Tegmen stout, penis guide parallel along 2/3 length, then gradually narrowing to apex in ventral view; penis guide in lateral view, widest at base, parallel along 2/5 length, then gradually narrowing to apex; parameres as long as penis guide with dense long setae on the inner sides and distal end with a group of long setae in lateral view (Fig. 2g–h).
Female genitalia: coxites distinctly elongate (Fig. 2i). Spermatheca approximately C-shaped, cornu without appendage.
Diagnosis
This species can be easily distinguished from other species of Priscibrumus by the following combination of characters: elytra reddish-brown, with a pair of black spots at elytral apex; parameres as long as penis guide.
Distribution
Kashmir, Nepal, India, Bhutan, Pakistan (Mulsant 1850, Crotch 1874, Gordon 1987, Barovskij 1922, Bielawski 1979, Miyatake 1985, Kovář 1997, Canepari 1997, Poorani 2002, Mader 1955) and Tibet, China (present study) (Fig. 4).
49BD6CF2-085C-5C68-A9A6-62CC9C552965BrumusMulsant, 1850Brumus Mulsant, 1850 in Mulsant 1850: 492.BrumusCoccinellaoctosignata Gebler, 1830Diagnosis
Brumus can be distinguished from other genera of the tribe Chilocorini by the following combination of characters: antenna 10-segmented, terminal antennomere very small and embedded in penultimate segment; pronotal basal margin entirely bordered with submarginal line; elytral epipleura narrow, more or less horizontal and without foveae; abdominal postcoxal lines complete; mid and hind tibiae with two apical spurs; tarsal claws without basal tooth, only slightly swollen at base.
TL: 3.80–4.00 mm, TW: 2.80–3.20 mm, TH: 1.87–2.07 mm, TL/TW: 1.32–1.35, PL/PW: 0.47–0.50, EL/EW: 1.05–1.10.
Body oval, moderately convex. Head, mouthparts and antenna brownish-yellow. Pronotum orange-yellow, with a black spot at centre of basal margin. Scutellum black. Elytra orange-yellow, with four pairs of black spots, the first one situated at the humeral angle; the second one situated at basal 2/5, near suture; the third one situated at basal 3/5, near outer margin; the fourth one situated basal 4/5, near suture (Fig. 3a–c). Underside orange-yellow, densely covered with short, greyish pubescence. Abdominal postcoxal lines complete and semicircular. Posterior margin of male abdominal ventrite 5 and of ventrite 6 distinctly emarginate (Fig. 3d).
Male genitalia: penis slender, penis capsule with short outer and long inner arm, apex of penis acute with membranous appendage (Fig. 3e–f). Tegmen stout, penis guide narrow at base, widest at basal 1/2, then gradually converging to blunt tip, symmetrical in ventral view; penis guide in lateral view, widest at base, parallel along basal 1/3, after that gradually converging to apex; parameres nearly as long as the penis guide with dense short setae on the inner sides and distal end with a group of short setae in lateral view (Fig. 3g–h).
Female genitalia: coxites distinctly elongate (Fig. 3i). Spermatheca approximately C-shaped, cornu without appendage.
Diagnosis
This species can be easily distinguished from other species of Brumus by the following combination of characters: pronotum with a black spot at central of basal margin; elytra orange-yellow, with four pairs of black spots; parameres nearly as long as the penis guide.
Distribution
Azerbaijan, Armenia, France, Italy, Russia: south European Territory, Afghanistan, Iran, Iraq, Kyrgyzstan, Mongolia, Tajikistan, Turkmenistan, Turkey, Uzbekistan (Gebler 1830, Motschulsky 1840, Mulsant 1850, Crotch 1874, Barovskij 1927, Mader 1955, Bielawski 1975, Bielawski 1984, Kovář 1997) and Xinjiang, China (present study) (Fig. 4).
ChecklistsChecklist of Chinese Chilocorini sensu Li et al. 2020C1D7BFF4-D441-5864-BA87-EA89FF4A197BBrumoidesChapin, 1965B90B50F7-8D94-52DA-91BD-3D699476C1B6BrumoideshainanensisMiyatake, 1970Distribution
China, Myanmar, Indonesia, India, Pakistan, Sri Lanka, Bangladesh, Australian, USA, Brazil and South Africa (Fabricius 1798, Crotch 1874, Poorani 2002, Kovář 2007, Li et al. 2018).
The genus Priscibrumus was erected by Kovář (1997), when he revised Exochomus and Brumus Mulsant, 1850 from the Palearctic Region. This revision was a huge contribution to our understanding of the relationships between Exochomus and its closely-related genera. Until now, Priscibrumus only contained seven species which mainly occur in the Pamir Mountains, especially in the western part of the Himalaya Mountains (Kovář 1997, Poorani 2002). Priscibrumushimalayensis (Kapur) was the first member of this genus recorded in China (Tibet) by Hu et al. (2013).
Brumus was considered a junior synonym of Exochomus by Ślipiński and Giorgi (2006). Subsequently, Kovář (2007) accepted this point of view and transferred all species of Brumus from the Palearctic Region to Exochomus. The recent phylogenetic studies of Chilocorini indicated that the relationship between Exochomus and Brumus is not so close. Exochomus is the sister group of a large clade containing various genera, for example, Xanthocorus, Priscibrumus, Parexochomus, Brumus and Brumoides, while Brumus is closely related to Brumoides (Li et al. 2020). Actually, Brumus can be easily distinguished from Exochomus by the following characters: elytral epipleuron more or less horizontal; tarsal claws without basal tooth, only slightly swollen at base. In Exochomus, the outer part of elytral epipleuron is distinctly descending; and the tarsal claws have a large triangular tooth at the base.
Acknowledgements
The research was supported by the National Natural Science Foundation of China (31802003, 31970441), Science and Technology Planning Project of Guangdong Province (2017A020208060) and the Science and Technology Program of Guangzhou (201804020070, 151800033).
ReferencesBarovskijV.1922Revisio specierum palaearticarum Coccinellidarum generis Exochomus Redtb.23289303BarovskijV.1927Revision specierum palaearticarum Coccinellidarum generis Brumus Muls. (Coleoptera).28194200BielawskiR.1975Ergebnisse der zoologischen Forschungen von Dr. Z. Kaszab in der Mongolei, Nr. 352. Coccinellidae V and VI (Coleoptera)2024727110.3161/00159301FF1975.20.16.247BielawskiR.1979Ergebnisse der Bhutan-Expedition 1972 des Naturhistorischen Museums in Basel. Coleoptera: Faro. Coccinellidae.483125BielawskiR.1984Coccinellidae (Coleoptera) of Mongolia.38281460CanepariC.1997Coccinellidae (Coleoptera) from the Nepal Himalayas.565165CaoC. Y.XiaoN. N.1984New species of Coccinellidae from Yunnan, China6109132ChineseChapinE. A.1965The genera of the Chilocorini (Coleoptera, Coccinellidae).13322727CheL. H.ZhangS. Q.LiY.LiangD.PangH.ŚlipińskiA.ZhangP.2017Genome-wide survey of nuclear protein-coding markers for beetle phylogenetics and their application in resolving both deep and shallow-level divergences1761342135810.1111/1755-0998.12664CrotchG. R.1874E. W. JansonLondon311EscalonaH. E.ZwickA.LiH. S.LiH. S.WangX. M.PangH.HartleyD.JermiinL. S.NedvědO.MisofB.NiehuisO.ŚlipińskiA.TomaszewskaW.2017Molecular phylogeny reveals food plasticity in the evolution of true ladybird beetles (Coleoptera: Coccinellidae: Coccinellini)17115110.1186/s12862-017-1002-3FabriciusJ. C.1798Proft and StorchCopenhagen572LedebourC. F.1830Bemerkungen über die Insecten Sibiriens, vorzüglich des Altai. (Part 3)GeblerA.G. ReimerBerlin427GoezeA. E.1777Weidmanns Erben und ReichLeipzig736GordonR. D.1985The Coccinellidae (Coleoptera) of America north of Mexico931912GordonR. D.1987A catalogue of the Crotch collection of Coccinellidae (Coleoptera).3146GorhamH. S.1892Coleoptera from Central China and the Korea2548185SchönherrC. J.1808[New taxa.]GyllenhalL.Eleutherata oder Käfer. Zweiter Theil. Sprechus-CryptocepalusC.F Marquard, Stockholm424GrayJ. E.1831Synopsis of the new species of Nepal insects in the collection of Major General HardwickeHopeF. W.Treutel, Wurtz and CoLondon40HuS. C.LinX. W.WangB. H.2013Henan Science and Technology Press213KamiyaH.1959A revision of the tribe Chilocorini of Japan and the Loochoos (Coleoptera: Coccinellidae)27299105KapurA. P.1958Coccinellidae of Nepal53309338JunkW.SchenklingS.1932Pars 120: Coccinellidae. II. pp. 225–659KorschefskyR.W. JunkBerlin10.1007/978-94-017-5006-6KovářI.1997Revision of the genera Brumus Muls. and Exochomus Redtb. (Coleoptera: Coccinellidae) of the Palaearctic region. Part I.445124LöblI.SmetanaA.2007New nomenclatorial and taxonomic acts and comments Coccinellidae, pp. 568–631KovářI.Apollo BooksStenstrup905ŁączyńskiP.TomaszewskaW.2012Chapinaria, new genus of Chilocorini for Endochilusmeridionalis Sicard from Africa (Coleoptera: Coccinellidae)6211910.3161/000345412X633658LinnaeusC.1758Holmiae: Laurentii Salvii823LiW. J.ChenX. S.WangX. M.RenS. X.2015Contribution to the genus Xanthocorus Miyatake (Coleoptera, Coccinellidae, Chilocorini)511899810.3897/zookeys.511.9584LiW. J.HuoL. Z.WangX. M.ChenX. S.RenS. X.2015The genera Exochomus Redtenbacher, 1843 and Parexochomus Barovsky, 1922 (Coleoptera: Coccinellidae: Chilocorini) from China, with descriptions of two new species91429130410.3956/2015-91.4.291LiW. J.HuoL. Z.AhrensD.RenS. X.WangX. M.2017Reniuscornutus, a new genus and species of Chilocorini from Tibet, China (Coleoptera, Coccinellidae)67812112810.3897/zookeys.678.11862LiW. J.HuoL. Z.ChenX. S.RenS. X.WangX. M.2017A new species of the genus Phaenochilus Weise from China (Coleoptera, Coccinellidae, Chilocorini)644334110.3897/zookeys.644.9825LiW. J.HuoL. Z.WangD.AhrensD.WangX. M.2018Contribution to the genus Chilocorus Leach, 1815 (Coleoptera: Coccinellidae: Chilocorini), with descriptions of two new species from China.46913410.5852/ejt.2018.469LiW. J.ŁączyńskiP.EscalonaH. E.EberleJ.HuoL. Z.ChenX. S.HuangW. D.ChenB. X.AhrensD.AhrensD.TomaszewskaW.WangX. M.2020Combined molecular and morphological data provide insights into the evolution and classification of Chilocorini ladybirds (Coleoptera: Coccinellidae)4544746310.1111/syen.12409MaderL.1955Evidenz der palearktischen Coccinelliden und ihrer Aberrationen in Wort und Bild. 2. Entomologische Arbeiten aus dem Museum G.67651035MagroA.LecompteE.MagneF.HemptinneJ.Crouau-RoyB.2010Phylogeny of ladybirds (Coleoptera: Coccinellidae): are the subfamilies monophyletic?5483384810.1016/j.ympev.2009.10.022MiyatakeM.1970The East-Asian coccinellid beetles preserved in the California Academy of Sciences1431956MiyatakeM.1985Coccinellidae collected by the Hokkaido University Expedition to Nepal Himalaya, 1968 (Coleoptera)30133MotschulskyV.1840Insectes du Caucase et des provinces transcasiennes recueillis et decrits (Continuation)13169180MotschulskyV.1853Diagnoses de Coléoptères nouveaux, trouvés par M.M. Tatarinoff et Gasckéwitsch aux environs de Pékin24451MulsantM. E.18502Annales des Sciencies Physiques et Naturelles, d’Agriculture et d’Industrie, publiées par la Société nationale d’Agriculture, etc.de Lyon, Deuxième Série1–1104MulsantM. E.1853Supplément à la Monographie des Coléoptères Trimerès Sécuripapes2129333PooraniJ.2002An annotated checklist of the Coccinellidae (Coleoptera) (excluding Epilachninae) of the Indian Subregion3630738310.1080/00305316.2002.10417335PooraniJ.RojeetT.2019New additions to Indian fauna of Coccinellidae (Coleoptera)10.1080/00305316.2018.1550448SasajiH.1968A revision of the Formosan Coccinellidae (II) tribes Stethorini, Aspidimerini and Chilocorini (Coleoptera)32124SeagoA. E.GiorgiJ. A.LiJ. H.ŚlipińskiA.2011Phylogeny, classification and evolution of ladybird beetles (Coleoptera: Coccinellidae) based on simultaneous analysis of molecular and morphological data6013715110.1016/j.ympev.2011.03.015SilvestriF.1909Nuovo Coccinellide introdotto in Italia7126129ŚlipińskiA.GiorgiJ. A.2006Revision of the Australian Coccinellidae (Coleoptera). Part 6. Tribe Chilocorini562265304ŚlipińskiA.2007ABRSCanberraArnettR. H.ThomasM. C.SkelleyP. E.FrankJ. H.2002CoccinellidaeVandenbergN. J.CRC PressBoca Raton10.1201/9781420041231WangX. M.RenS. X.2010Two new species of Chujochilus Sasaji, 2005 (Coleoptera: Coccinellidae) from China6033193210.3161/000345410X535316WeiseJ.1885Beschreibung einiger Coccinelliden46227241WeiseJ.1887Neue sibirische Chrysomeliden und Coccinelliden nebst Bemerkungen über früher beschriebene Arten531164238WeiseJ.1895Neue Coccinelliden, sowie Bemerkungen zu bekannten Arten39120146WeiseJ.1898Üeber bekannte und neue Coccinelliden641225238ZaslavskijV. A.1962Novyy palearkticheskiy vid Chilocorus (Coleoptera, Coccinellidae)41398401240B1A68-AF3A-558F-8095-F8765A992C1F10.3897/BDJ.8.e51092.figure13926958
Priscibrumusdisjunctus Canepari, 1997. a. dorsal view; b. lateral view; c. frontal view; d. abdomen; e. penis; f. apex of penis; g. tegmen, lateral view; h. tegmen, ventral view. Scale bars: 0.1 mm.
Priscibrumusuropygialis (Mulsant, 1853). a. dorsal view; b. lateral view; c. frontal view; d. abdomen; e. penis; f. apex of penis; g. tegmen, lateral view; h. tegmen, ventral view; i. ovipositor. Scale bars: 0.1 mm.
Brumusoctosignatus (Gebler, 1830). a. dorsal view; b. lateral view; c. frontal view; d. abdomen; e. penis; f. apex of penis; g. tegmen, lateral view; h. tegmen, ventral view; i. ovipositor. Scale bars: 0.1 mm.