Corresponding author: Ji C. Kang (
Academic editor: Danny Haelewaters
Species of the fungal genus
In this study, a new species,
The genus
Species of
There are 92 species epithets for
Fresh samples of dead culms of
Conidiomata present on the surface of the host were observed using a stereomicroscope (Motic SMZ-171, Wetzlar, Germany). Sections of conidiomata were taken and mounted in water on a microscope slide to observe fungal characters. Photographs were taken using a Nikon ECLIPSE Ni-U compound microscope connected with a Nikon camera series DS-Ri2. Morphological structures (conidiophores, conidiogenous cells, conidia) were measured by Image Frame Work software v. 0.9.7. Adobe Photoshop CC 2019 was used for editing the photographic plate. Colonies were described, based on the colour charts of
Genomic DNA was extracted from fresh mycelia obtained from living cultures that were grown on potato dextrose agar (PDA) for 15 days at room temperature, using the EZgene Fungal gDNA Kit (GD2416, Biomiga, San Diego, California, USA) following the manufacturer’s instructions. PCR amplification was done for the internal transcribed spacer region (ITS), the large subunit of the ribosomal RNA gene (LSU), translation elongation factor 1-alpha (TEF1) and beta-tubulin (TUB2). The following primers were used: ITS5 and ITS4 for ITS (
PCR amplification was done in 50-μl volumes consisting of 2 μl of DNA template, 2 μl of each 10 μM forward and reverse primers, 25 μl of 2 ×Taq PCR Master Mix and 19 μl of deionised water. Cycling conditions were as follows: for ITS: initial denaturation at 94°C for 5 min, then 35 cycles of denaturation at 94°C for 45 s, annealing at 52°C for 50 s and extension at 72°C for 1 min; and final extension at 72°C for 10 min. For LSU: initial denaturation at 94°C for 5 min, then 35 cycles of denaturation at 94°C for 45 s, annealing at 52°C for 50 s and extension at 72°C for 1 min; and final extension at 72°C for 10 min. Lastly, for TEF1: initial denaturation at 94°C for 5 min; then 35 cycles of denaturation at 94°C for 1 min, annealing at 56°C for 1 min and extension at 72°C for 90 s; and final extension at 72°C for 10 min.
PCR products were checked in 1% agarose gels and sent to Sangon Biotech (Shanghai) Co. Ltd, China for sequencing, using the same primers.
Raw sequence reads were combined using BioEdit v. 7.0.5.3 (
Phylogenetic analyses were performed using the CIPRES Science Gateway web portal (
BI analysis was performed using the MrBayes on XSEDE tool available on the CIPRES Science Gateway (
colonies flat, spreading, with moderate, pale, aerial mycelium. On PDA, surface white, lightly yellow with patches of dirty white, reverse lightly pigmented.
FoF 09162
Referring to the host from which the holotype was isolated, a member of the bamboo subfamily (
The concatenated ITS–LSU–TEF1–TUB2 dataset consisted of 98 taxa with
The family
ITS sequence data provide limited resolution to distinguish species for some
To date, seven species of
This work was funded by grants of the National Natural Science Foundation of China (NSFC Grants nos. 31670027 & 31460011). The authors are grateful to the Thailand Research Fund grant “Impact of climate change on fungal diversity and biogeography in the Greater Mekong Sub-region” (RDG6130001). Shaun Pennycook is thanked for his suggestions on naming the new fungus. The authors would also like to thank Mae Fah Luang University.
Figs
Figure 2 Continued.
Details of fungal taxa used in this study. Newly-generated sequences are indicated by ▲ after the species name; type materials are in bold.
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S-642 | Submerged wood | China |
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- | - |
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CBS 133509 | USA |
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CBS 449.92 | Bamboo | Canada |
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- | Japan |
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Undetermined |
Spain |
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Leaves of bamboo | China |
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LC7124 | Leaves of bamboo | China |
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Culms of |
Thailand |
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- |
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China |
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LC8181 |
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China |
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CBS 145127 | Dead leaves of |
China |
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China |
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- |
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CFCC 53037 |
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China |
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- |
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Thailand |
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- |
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Dead culms of |
Spain |
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China |
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LC8175 |
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China |
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Dead culms of |
Spain |
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IMI 285638b | Bangladesh |
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- | |
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Living leaves and culms of |
China |
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- |
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CFCC 52302 | Living leaves and culms of |
China |
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- |
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Dead culms of bamboo | China |
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- | - |
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LC5318 | Air in karst cave | China |
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Air in karst cave | China |
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Gut of a grasshopper | India |
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Beach sand | Spain |
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- |
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Culms of |
China |
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KUMCC 16-0204 | Dead culms of bamboo | China |
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- | - |
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Culms of |
China |
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KUMCC 16-0201 | Culms of bamboo | China |
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CBS 145133 |
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Spain |
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Bamboo | New Zealand |
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Dead culms of |
Portugal |
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Dead culms of |
Italy |
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CBS 145139 | Dead culms of |
Spain |
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- |
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IFO30500 | - | Japan |
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IFO 31098 | Leaves of |
Japan |
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- |
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India |
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AMH-9556 |
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India |
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LC4494 | China |
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China |
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CBS 113332 | Dead culms of |
South Africa |
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Dead culms of |
South Africa |
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LC11688 | Bee bread | China |
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- |
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Hive-stored pollen of |
China |
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- |
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MFLUCC 11-0479 | Dead culms of bamboo | Thailand |
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- | - |
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Dead culms of bamboo | Thailand |
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- | - |
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Malaysia |
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Beach sands | Spain |
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Air | Spain |
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- |
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AP25418 | Leaves of |
China |
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CBS 145131 | Dead leaves of |
Germany |
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Dead blades of |
India |
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Bamboo | China |
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- | - |
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JHB006 | Dead culms of bamboo | China |
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- | - |
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Bamboo | China |
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- | - |
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China |
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LC8177 | China |
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China |
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Dead culms of bamboo | Thailand |
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CBS 114317 | Leaves of |
Iran |
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CBS 114318 | Leaves of |
Iran |
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Culms of |
Italy |
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Dead culms of |
China |
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Dead culms of |
Spain |
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- |
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Leaves of bamboo | China |
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LC8173 | Leaves of bamboo | China |
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Leaves of bamboo | Netherlands |
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- |
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Malaysia |
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CBS 123185 | Leaves lesion of |
New Zealand |
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- |
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Leaves of |
Australia |
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CBS 549.86 | Leaves of |
Germany |
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- |
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Dead culms of |
China |
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- |
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CFCC 52304 | Dead culms of |
China |
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- |
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LC8179 |
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China |
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Soil | Norway |
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CBS 372.67 | Air | - |
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CBS 664.74 | Soil under |
Netherlands |
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CBS 191.73 | Air | Netherlands |
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CBS 831.71 | - | Netherlands |
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Food, pharmaceutical excipients, atmosphere and home dust | Spain |
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- |
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Dead branches of bamboo | China |
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- |
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CBS 145154 | Dead leaves of |
Spain |
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Dead culms of bamboo | Thailand |
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LC7291 | Leaves of bamboo | China |
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Leaves of bamboo | China |
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MFLUCC 15-0199 | Dead culms of bamboo | Thailand |
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- | - |
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Dead culms of bamboo | Thailand |
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- | - |
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Dead branches of |
China |
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- |
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CFCC 53039 | Dead branches of |
China |
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IMI 326344 | - | - |
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- |
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Vietnam |
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- |
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Soil from roadway | Zimbabwe |
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CBS 595.66 | Soil | Austria |
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- | - |
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DDQ00281 | Dead culms of |
China |
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- | - |
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Dead culms of |
China |
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- | - |
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UK |
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CPC 19965 |
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UK |
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Morphological comparison of the seven
Morphological comparison
The morphological comparison of seven
File: oo_453721.txt
BI output file
Phylogenetic output file for BI
The output file of BI
File: oo_478675.txt
MP output file
The phylogenetic output file of MP
File: oo_478682.txt
The output file of ML
The phylogenetic output file of ML
File: oo_478686.txt