Corresponding author: Yong Wang (
Academic editor: Danny Haelewaters
Phylogenetic analyses of a combined ITS–LSU–
In recent years, most species of
The samples were collected in 2017 at the Medicinal Botanical Garden, Nanning, Guangxi, China. Single spore isolates were obtained on oatmeal agar (OA), malt extract agar (MEA) and potato dextrose agar (PDA), followed by incubation at 25 °C. Colony diameters were measured after 1 week (
Fungal mycelia were scraped off the surface of the pure culture plate with a sterile scalpel. Total genomic DNA was extracted using the A BIOMIGA Fungus Genomic DNA Extraction Kit (GD2416, BIOMIGA, San Diego, California, USA). Four loci of each fungal strains were amplified, including the internal transcribed spacer (ITS) region with primers V9G (
The related DNA sequences for phylogenetic analyses in this study were downloaded from GenBank (Table 1). Amongst them,
Pathogenic on the leaf spot of
Culture characteristics: Colonies on PDA, 46−57 mm diameter after 1 week, irregular at margin, aerial mycelia floccose, grey with a white margin, brown near the centre; reverse pale brown, with a white margin. Colonies on MEA 44−47 mm diameter after 1 week, regular at margin, covered by brown, dense aerial mycelia, yellow near the centre; reverse greyish-brown. Colonies on OA, 41−46 mm diameter after 1 week, irregular at margin, covered by white aerial mycelia sparse, brownish, reverse buff to yellowish-olivaceous.
In reference to the host (
Pathogenic on the leaf spot of
Culture characteristics: Colonies on PDA, 46−50 mm diameter after 1 week, regular at margin, densely covered by floccose aerial mycelia, grey, with a white concentric ring near the margin; reverse pale black, with a white concentric ring near the margin. Colonies on MEA, 52−58 mm diameter after 1 week, regular at margin, dull green, aerial mycelia floccose, aerial mycelia sparsely, grey near the centre; reverse changing towards margin from the centre greyish-brown to brown. Colonies on OA 34−47 mm diameter after 1 week, irregular at margin, covered by floccose aerial mycelia, mycelia sparse in some furrowed zone, reverse buff to pale olivaceous.
Phylogenetic analyses (Fig.
We would like to thank Dr. Danny Haelewaters and Ms. Subodini N. Wijesinghe for their meticulous reviews. This research is supported by the following funding sources: National Natural Science Foundation of China (nos. 31972222, 31660011), Program of Introducing Talents of Discipline to Universities of China (111 Program, D20023), Talent Project of Guizhou Science and Technology Cooperation Platform ([2017]5788-5, [2019]5641, [2019]13), Guizhou Science, Technology Department of International Cooperation Base Project ([2018]5806), the Project of Guizhou Provincial Education Department ([2020]001) and Guizhou Science and Technology Innovation Talent Team Project ([2020]5001). Nalin N. Wijayawardene acknowledges the National Natural Science Foundation of China (No: 31950410558) and Guizhou Medical University (grant number FAMP201906K).
Phylogenetic tree inferred from a Maximum Parsimonious analysis, based on a concatenated alignment of ITS,
Sequences that were used for phylogenetic analysis. The accession numbers in bold are those generated in this study. Ex-type strains are marked by an asterisk (*).
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CBS 120422 |
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CBS 124673 |
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CBS 142453* |
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CBS 142859 |
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CBS 142860 |
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CBS 124.93 |
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CBS 325.82* |
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CBS 506.91* |
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CBS 497.91 |
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CGMCC 3.18114* |
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CGMCC 3.18115 |
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CGMCC 3.18116 |
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CBS 268.93* |
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CBS 108.93 |
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CGMCC 3.19245* |
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GUCC2070.6 |
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GUCC2070.3 |
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MFLU 17-2281 |
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GUCC2070.7 |
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CBS 436.75* |
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CBS 131.93 |
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CBS 229.32 |
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ICMP 16850 |
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CBS 562.81* |
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DNA base differences amongst our strains and related species in four gene regions.
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GUCC 2070.7 | 0 | 0 | 0 | 0 |
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CBS 268.93 * | 21 | 39 | 26 | 0 |
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CBS 325.82* | 6 | 1 | 9 | 0 |
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GUCC 2070.3 | 0 | 0 | 0 | 0 |
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GUCC 2070.6 | 5 | 0 | 1 | 4 |
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CGMCC 3.19245* | 1 | 0 | 1 | 3 |
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MFLU 17-2281 | 4 | 55 | 1 | 6 |
The pycnidia, conidiogenous cells, and conidia morphology of the new species compared to known species of
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(sub-)globose to ellipsoidal, whitish at onset | 205−635 × 195−510 | 3.5−6.5 × 4.5−9 | oblong, with both ends rounded, hyaline, smooth and thin-walled, aseptate | 3−4.5 × 1.5−2.3 |
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(sub-)globose to ellipsoidal | 130−400 × 120−370 | 5−7 × 5.5−9 | oblong with both ends rounded or ovoid, smooth and thin-walled, hyaline, aseptate | 3.5−5.5 × 2−3 |
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glabrous, ovoid | 120−210 × 90−140 | 3.5−4 × 4.5−5 | aseptate, hyaline, smooth and thin-walled, cylindrical | 3−4 × 2 |
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(sub-)globose with 1−2 narrow and long necks | 125 × 102 | - | oblong to ellipsoidal | 3.3−8 × 2.2−3.3 |
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globose | 250 × 70 | 5−7 × 4−8 | oblong to ellipsoidal | 4−6.5 × 2–3 |
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globose to broadly ellipsoidal | 150−280 × 150−220 | 4−5.5 × 3−4.5 | ellipsoidal to ovoid or slightly allantoid | 3.5−4.5 × 1.8−2.5 |
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globose to flask-shaped | 30−150 × 28−120 | 3−4.5 × 3.5−4.5 | ellipsoidal to oblong | 2.5−4 × 1.5−2.5 |
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globose to subglobose | 50−440 × 145−420 | 4.5−7 × 3.5−6.5 | oblong to cylindrical | 3−4.5 × 1.5−2.5 |
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subglobose | 115−245 × 85−230 | 2.5−3.5 × 2−3 | ellipsoidal to ovoid or slightly allantoid | 3.5−5.5 × 1.5−2.5 |
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globose to subglobose, brown, glabrous | 60−330 × 67−280 | 6−10 × 3−6 | oval to oblong, occasionally bacilliform | 3−5.5 × 1.5−2 |
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glabrous, ovoid | 70−90 × 68−85 | 3−6 × 4−5 | hyaline, cylindrical, aseptate | 3−4 × 2−3 |
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subglobose to irregular, dark brown | 39−200 × 48−230 | 4.5−7 × 4−5 | oblong to cylindrical, incidentally slightly obovoid | 3−5 × 1.5−2.5 | this study |
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subglobose | 100−300 | 2−6 × 3−6 | ellipsoidal | 3−4 × 1−2 |
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subglobose or depressed | 90−180 | - | oval or ellipsoidal | 4−7 × 2.5−3.5 |
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