Two new species of the genus Anufrievia Dworakowska from China (Hemiptera: Cicadellidae: Typhlocybinae)

Abstract Background The leafhopper genus Anufrievia Dworakowska, 1970 includes 33 species and is widely distributed in China, Korea, South Korea, Japan, Nepal, India, Thailand and Vietnam. New information Two new species found at Bijie City and Shibing County, Guizhou Province, China are described and illustrated, A. crispata sp. nov. and A. confluensa sp. nov. A key to distinguish the Chinese species of the genus is given.


Introduction
The leafhopper genus Anufrievia Dworakowska, 1970 belongs to the tribe Erythroneurini of Typhlocybinae, with Anufrievia rolikae Dworakowska, 1970 as its type species (Dworakowska 1970). The genus previously contained 33 species, including 28 species in China (Cao et al. 2018). In this paper, two new species from Guizhou Province, China are described and illustrated and a key to distinguish the Chinese species of the genus is provided.
The characteristics of Anufrievia genus are as follows. Body yellow or white, often with brown markings or diffuse patterns. Head slightly narrower than pronotum. Length of crown distinctly shorter than inter ocular width. Body length 2.0-4.0 mm (including wing). Vertex anterior margin with minute paired black spots. Male anteclypeus narrow and flat, greyish, brown or black. Pronotum pale, scutellum with dark lateral triangles. Forewing with 4 apical cell small, not reaching apex of forewing, 2 apical cell nearly rectangular and 1 apical cell broad. Hind wing venation follows typical schemes for Erythroneurini taxa. Abdominal apodemes small and narrow, extended dorsomesad.
Male pygofer lobe with hind margin sleeked or truncated slightly, basal lateral angle usually with macrosetae, sometimes absent and scattered a few fine setae in outer lateral surface. Pygofer dorsal appendage articulated to pygofer lobe with ventral appendage absent. Subgenital plate with some macrosetae in mid-ventral part, broad basally and sometimes terminal half tapering abruptly; row of stout setae along upper margin from sub-base to apex. Apex of style pointed, bifid, foot-shaped or otherwise modified. Aedeagus with dorsal apodeme well developed; aedeagal shaft tubular; gonopore sub-basal to subapical on ventral surface. Connective lateral arms long, Y-or V-shaped.

Materials and methods
All specimens in this study were collected by the sweeping-net method. Morphological terminology used follows Dietrich (2005) and Dworakowska (1993). An Olympus SZX16 dissecting microscope was used for observing and an Olympus BX53 stereomicroscope for drawing. A KEYENCE VHX-5000 digital microscope was used for taking habitus photos. Body measurements are from the apex of the vertex to the tip of the forewing. All specimens examined were deposited in the collection of the School of Karst Science, Guizhou Normal University, China (GZNU).

Etymology
The new species is named from the Latin word "crispatus", referring to the serrated marginal lamellae on both sides of shaft apex dorsad ( Fig. 2F and G).

Taxon discussion
This species can be distinguished from other species in this genus by the unique characters of the aedeagus: the aedeagal shaft with serrated marginal lamellae on both sides of apex, pair of small curved processes subapically; short dorsal apodeme and long prearium.

Description
Male length 2.8 mm (including wing). Body yellowish. Vertex brownish-yellow, with pair of small dark brown apical spots ( Fig. 3A and C). Eyes grey. Face pale milky yellow, anteclypeus and frontoclypeus light brownish ( Fig. 3B and D). Pronotum and scutellum brownish-yellow and anterior margin of scutellum with black triangles (Fig. 3A and C). Forewing beige (Fig. 3A and B). Abdominal apodemes very short, not extended to 3 sternite (Fig. 4A).

Diagnosis
Male genitalia. Pygofer lobe broad, with dense microsetae near dorso-caudal margin and several peg-like setae on outer surface (Fig. 4B). Pygofer dorsal appendage broadened at base, tapering towards apex (Fig. 4C). Subgenital plate slightly concave near middle area, with three macrosetae on lateral margin, row of short stout setae along upper margin from sub-base to apex (Fig. 4D). Style with two points at apex; pre- Two new species of the genus Anufrievia Dworakowska from China (Hemiptera: ... apical lobe small (Fig. 4E). Aedeagal shaft straight and flat in lateral view, but long and slim in ventral view; gonopore arising from ventral surface, reaching two thirds of aedeagal shaft; dorsal apodeme well developed ( Fig. 4F and G). Connective Y-shaped, two arms slender, central lobe absent (Fig. 4H).

Etymology
The new species is named from the Latin word "confluensus", referring to the connective stem fused with a long process (Fig. 4H).

Taxon discussion
This species is similar to A. akazu (Matsumura 1932), but can be recognised by the subapical ventral surface without paired short processes and pygofer appendage not bifurcate at apex.