Corresponding author: Olivier Raspé (
Academic editor: Yasen Mutafchiev
The bolete specimens, collected in forests dominated by
The plant family
In this study, we describe a new species of
Fresh basidiomata were collected during the rainy season (2019) from Chiang Mai and Chiang Rai Provinces, orthern Thailand. The basidiomata were photographed on-site and wrapped in aluminium foil. The descriptions of the macroscopic features were made on the same day, after which the basidiomata were dried in an electric drier at 45–50°C. Specimens were deposited in the Mae Fah Luang University (MFLU) or CMUB Herbaria.
The habitat, locality information and macro-chemical reactions on fresh basidiomata were recorded. Spore prints were taken for each collection. Colour codes were given using
Genomic DNA was extracted from CTAB-preserved tissues or dry specimens (ca. 10 mg) using a CTAB isolation procedure, adapted from
The sequences were assembled using Geneious 8 (Biomatters). The Basic Local Alignment Search Tool (BLAST) (
All analyses were done on the CIPRES Science Gateway (
Using jModeltest2 (
This species is distinguished from
Epithet “
Thus far known only from northern Thailand.
Solitary, in tropical forest dominated by
Morphologically,
The basidiospores of
The concatenated gene dataset comprised 47 terminals. The final alignment contained 121 sequences (38 for
Our survey on the diversity of boletes in northern Thailand led to the discovery of a second species of
B. Raghoonundon appreciates the kind support given by the Mushroom Research Foundation (MRF), Thailand. The authors thank the Thailand Science Research and Innovation (TSRI) (Grant No. DBG6280009) entitled “Macrofungi diversity research from the Lancang-Mekong Watershed and surrounding areas”. The authors are also grateful to Amy Choong for loaning her collection of
Photograph of
Microscopic features of
Maximum Likelihood phylogenetic tree inferred from the three-gene dataset (atp6, rpb2, tef1). The three
List of collections used for DNA analyses, with origin, GenBank accession numbers and reference(s).
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OR0209 | Thailand |
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HKAS75739 | China | – |
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BOTH4144 | USA |
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OR0345 | Thailand |
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LEE1180 | Malaysia |
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OR0228 | China |
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BOTH4591 | USA |
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BOTH4432 | USA |
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HKAS76669 | China | – |
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HKAS63502 | China | – |
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RW105a | Belgium |
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VDKO0938 | Belgium |
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VDKO1128 | Belgium |
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HKAS57758 | China | – |
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VDKO0844 | Belgium |
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HKAS76658 | China | – |
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OR0082 | Thailand |
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This study |
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VDKO1006 | Belgium |
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HKAS90639 | China | – |
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HKAS53427 | China | – |
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OR0711 | Thailand |
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KPM-NC 17793 | Japan |
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– |
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KPM-NC 17797 | Japan |
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– |
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KPM-NC 17748 | Japan |
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– |
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MEL 2341996 | Australia |
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– | |
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MES270 | USA |
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– | |
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OR0477 | China |
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This study |
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OR0570 | Thailand |
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HKAS 52680 | China | – |
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OR0231 | China |
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OR0738 | Thailand |
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MB03-079 | USA |
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OR0278 | China |
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BC0179 | Thailand |
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OR049 | Thailand |
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MBsn | USA |
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SV170 | Thailand |
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TUMH-40252 | Japan |
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– |
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TUMH-40266 | Japan |
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– |
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A.W. Claridge 2137 | Australia |
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– |
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DED7873 | Thailand |
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ACMF5 | Singapore |
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This study |
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KPM-NC 18012 | Japan |
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– |
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KPM-NC 18001 | Japan |
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– |
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Muroi361 | USA |
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HKAS50281 | China | – |
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OR622 | Thailand |
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– |
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This study |