Male (holotype, DUIER-MWa11, Fig. 1). Total length 24.56: cephalothorax 10.89 long, 6.98 wide; opisthosoma 8.14 long, 5.64 wide. Carapace dark, gray hairs. Fovea concave. Both eye rows recurved. Eye sizes and inter-distances: AME 0.55, ALE 0.64, PME 0.48, PLE 0.49; ALE-AME 0.14, AME-AME 0.17, ALE-PLE 0.18, PLE-PME 0.07, PME-PME 0.88. Eye area 1.11 long, 2.21 wide. Chelicerae black, with 15 stout promarginal teeth, 13 small retromarginal teeth, and 15 tiny teeth within fang furrow (Fig. 1F). Labium and maxillae chestnut, with grey bristles and patch of cuspules on anterior half part (Fig. 1K); maxillae area length 2.58, and cuspules area length 1.45. Sternum with three pairs of sigilla (Fig. 1J). Palp: tibia grey, 4.34 long, with four long prolateral spines (Fig. 1G), palpal trochanter without lyral spines; embolus 4.76 long, apex curved and hook shaped (Fig. 1B-E).

Leg spines. I: femur with 1 prolateral near base; patella 2 prolateral distally; tibia 2 prolateral, 2 retrolateral, 9 ventral (Fig. 2A); metatarsus with 19 ventral spines (Fig. 2B), and two comb-like organs near distad, among them, prolateral with 2 spines, retrolateral with 3 spines (Fig. 3A-B); tarsus with 3 prolateral and 3 retrolateral. II: femur 1 prolateral near distad; patella 2 prolateral; tibia 1 prolateral, and 3 ventral, 1 spine near distad, 2 near basal (Fig. 2C); metatarsus with 1 prolateral, 10 ventral (Fig. 2D), two comb-like organs near distad, among them, prolateral with 3 spines, retrolateral with 4 spines (Fig. 3C-D); tarsus with 6 prolateral, 6 retrolateral. III: femur 1 prolateral and 1 retrolateral; patella 2 prolateral, 1 retrolateral; tibia 3 prolateral, 2 retrolateral, and 3 ventral; metatarsus 2 ventral, 4 prolateral, 3 retrolateral, two comb-like organs near distad, among them, prolateral with 3 spines, retrolateral with 4 spines (Fig. 3E-F); tarsus with 4 prolateral, 5 retrolateral. IV: femur 1 prolateral and retrolateral near distal end; patella with 2 prolateral, 1 retrolateral; tibia with 2 prolateral, 1 retrolateral, 3 ventral near distal end; metatarsus with 2 dorsal, 3 prolateral, 3 retrolateral, 7 ventral, and two comb-like organs near distad, among them, prolateral with 3 spines, retrolateral with 5 spines (Fig. 3G-H); tarsus with 7 prolateral, 6 retrolateral.

Measurements of palp and legs: palp: 9.63 (2.28, 6.12, 1.23); I: 22.79 (6.14, 7.66, 5.58, 3.41); II: 25.80 (6.72, 8.75, 6.84, 3.49); III: 24.69 (6.14, 7.89, 6.93, 3.73); IV: 29.75 (7.37, 9.35, 8.68, 4.35). Leg formula: 4231.

Abdomen gray brown, hairy. Spinnerets, PMS one segment, 1.87 long, 0.48 wide, PMS-PMS 0.78; PLS three segments, PLS 10.65 long (3.20, 3.19, 4.26).

Female (DUIER-MWa21, Fig. 4). Total length 28.39 : cephalothorax 11.67 long, 6.66 wide; opisthosoma 9.11 long, 6.54 wide. Eye sizes and inter-distances: AME 0.52, ALE 0.71, PME 0.50, PLE 0.51; AME-AME 0.26, ALE-AME0.24, ALE-PLE 0.12, PME-PME 0.91, PLE-PME 0. Eye area 1.13 long, 2.24 wide. Chelicerae brown, with 15 stout promarginal teeth, 12 small retromarginal teeth, and 13 tiny teeth within fang furrow (Fig. 4B). Labium and maxillae dark yellow, with dark brown bristles and patch of cuspules on anterior half part (Fig. 4C); maxillae area length 2.34, and cuspule area length 1.58. Sternum with three pairs of sigilla (Fig. 4D); palpal trochanter without lyral spines.

Leg spines. I: femur with 1 prolateral near distad; patella 1 prolateral; tibia with 1 prolateral; metatarsus with 7 ventral, and two comb-like organs near distad, prolateral with 3 spines, retrolateral with 2 spines (Fig. 5A-B); tarsus with 3 prolateral, 4 retrolateral. II: femur 1 prolateral near distad; patella 2 prolateral; tibia 1 prolateral, 2 ventral; metatarsus 1 prolateral, 6 retrolateral, and two comb-like organs near distad, among them, prolateral with 3 spines, retrolateral with 4 spines (Fig. 5C-D); tarsus with 5 prolateral, 3 retrolateral. III: femur with 1 dorsal; patella 2 prolateral and 1 retrolateral; tibia 3 prolateral, and 2 retrolateral, 3 ventral distally; metatarsus with 2 dorsal, 5 prolateral, 4 retrolateral, 6 ventral, and two comb-like organs near distad, among them, prolateral with 3 spines, retrolateral with 3 spines (Fig. 5E-F); tarsus with 5 prolateral, 6 retrolateral. IV: femur 1 prolateral near distad, 1 retrolateral; patella 2 prolateral, 1 retrolateral; tibia 3 prolateral, and 2 retrolateral, 3 ventral near distad; metatarsus with 2 dorsal, 3 prolateral, 4 retrolateral, 7 ventral, and two comb-like organs near distad, prolateral with 3 spines, retrolateral with 6 spines (Fig. 5G-H); tarsus with 9 prolateral, 7 retrolateral.

Measurements of palp and legs: palp: 12.07 (3.07, 4.80, 4.20); I: 20.65 (5.80, 7.47, 4.85, 2.53); II: 21.99 (5.81, 7.92, 5.33, 2.93); III: 22.24 (5.39, 7.25, 6.33, 3.27); IV: 25.37 (6.24, 8.35, 7.54, 3.24). Leg formula: 4321.

Receptacula apically teardrop shaped, the ratio of the length of the T to the length of the CD is almost 1:6 (Fig. 6A-B). Spinnerets: PMS one segment, PMS 2.78 long, 0.58 wide, PMS-PMS 1.63; PLS three segments, PLS 11.11 long (2.91, 3.75, 4.45).

Males of Macrothele washanensis sp. n. resemble M. arcuata Tang, Zhao & Yang, 2020 by having similar bulb shape, but they can be distinguished by the BH no protrusion in prolateral view, embolus tapers from base to apex, and hook-shaped, the ratio of the length of the BH to the length of the E is almost 1 : 4 (Fig. 1B-E); the four tibial spines visible in prolateral view (Fig. 1G-I); tibia I with nine spines visible in ventral view, tibia II straight, with three ventral spines (Fig. 2) (vs tibia with three prolateral spines, and three ventral spines, embolus with visible protrusion, joint of embolus and bulb is strongly bent, embolus needle shaped, the ratio of the length of the BH to the length of the E is almost 1 : 5; tibia I with 26 spines, tibia II with retrolateral bend and 15 ventral spines in M. arcuata). Females of M. washanensis sp. n. can be differentiated from M. arcuata by the receptacula apically teardrop shaped, the ratio of the length of the T to the length of the CD is almost 1 : 6 (Fig. 6) (vs copulatory duct long, shape of the English letter “G”; receptacula apically oval, the ratio of the length of the T to the length of the CD is almost 1 : 8 in M. arcuata).

The species epithet is a noun in apposition referring to the type locality.

China, Yunnan Province (Cangyuan, Gengma) (Fig. 15)

Spinning large funnel web on crevices. Female often stays in the entrance of funnel tube, when the sheet part of funnel web was hit by other animals, she quickly rush out, to catch the prey, or attack the enemy (Fig. 7B). If the male of the same species comes, releasing some chemical clue or sending vibration via the web, female accepted the clue and walk out for further communication and copulation (Fig. 7A). After some days of copulation, female will lay several dozens of eggs, packaged in a silk-sac, then carried it under her ventral side with cheliceral fang (Fig. 7C).

Male (holotype and two male paratypes, n = 3): total length 23.30-24.56; maxillary cuspules 220-269, labial cuspules 53-59. Female (paratypes, n = 3): total length 23.43-28.39; maxillary cuspules 260-297, labial cuspules 61-70.

Male (holotype, DUIER-MWl11, Fig. 8). Total Length 22.52: cephalothorax 11.43 long, 7.49 wide; opisthosoma 8.49 long, 4.97 wide. Carapace dark chestnut, gray hairy. Fovea concave. Both eye rows recurved. Eye sizes and inter-distances: AME 0.47, ALE 0.62, PME0.45, PLE 0.50; ALE-AME 0.15, AME-AME 0.31, ALE-PLE 0.22, PLE-PME 0.05, PME-PME 0.95. Eye area 1.15 long, 2.28 wide. Chelicerae dark, with 13 stout promarginal teeth, 15 small retromarginal teeth, 17 tiny teeth with fang furrow (Fig. 8F). Labium and maxillae chestnut, with dark brown bristles and patch of cuspules on anterior half part (Fig. 8J); maxillae area length 3.30, cuspules area length 1.48. Sternum chestnut, and with three pairs of sigilla (Fig. 8K). Palp: tibia yellow, 4.34 long，with one long and two stout prolateral spines, two stout dorsal spines (Fig. 8G-I), palpal trochanter without lyral spines; embolus terminal end curved, 4.76 long. (Fig. 8E)

Leg spines. I: femur with 1 dorsal near distad; tibia 2 prolateral, 3 retrolateral, 10 ventral (Fig. 9A) metatarsus with 16 ventral, 12 ventral on near based (Fig. 9B), and two comb-like organs near distad, among them, prolateral with 2 spines, retrolateral with 3 spines (Fig. 10A-B); tarsus 3 prolateral, 4 retrolateral. II: femur with 2 dorsal; patella 2 prolateral; tibia 1 prolateral, 7 ventral (Fig. 9C); metatarsus with 1 prolateral, 7 ventral (Fig. 9D), and two comb-like organs near distad, prolateral with 2 spines, retrolateral with 3 spines (Fig. 10C-D); tarsus 4 prolateral, 4 retrolateral. III: patella 2 prolateral, 1 retrolateral; tibia 3 prolateral, 1 retrolateral, 4 ventral; metatarsus 2 dorsal, 4 prolateral, 2 retrolateral, 7 ventral, and two comb-like organs near distad, among them, prolateral with 3 spines, retrolateral with 2 spines (Fig. 10E-F); tarsus with 7 prolateral, 5 retrolateral. IV: femur with 1 dorsal near basad; patella 2 prolateral, 1 retrolateral; tibia 2 prolateral, 2 retrolateral, 4 ventral; metatarsus 1 dorsal, 4 prolateral, 3 retrolateral, 7 ventral, and two comb-like organs near distad, among them, prolateral with 3 spines, retrolateral with 5 spines (Fig. 10G-H); tarsus with 7 prolateral, 7 retrolateral.

Measurements of palp and legs: palp: 12.30 (5.07, 6.22, 1.01); I: 22.44 (6.04, 7.81, 5.82, 2.77); II: 23.40 (6.09, 7.67, 6.29, 3.35); III: 22.77 (5.99, 7.38, 6.06, 3.34); IV: 27.76 (6.99, 8.75, 8.44, 3.58). Leg formula: 4231.

Abdomen black, hairy. Spinnerets: PMS one segment, 1.84 long, 0.45 wide, PMS-PMS 0.86; PLS three segments, PLS 10.35 long (3.29, 3.55, 3.51).

Female (DUIER-MWl21, Fig. 11). Total length 26.86: cephalothorax 8.95 long, 6.77 wide; opisthosoma 8.92 long, 5.18 wide. Eye sizes and inter-distances: AME 0.58, ALE 0.70, PME 0.50, PLE 0.56; AME-AME 0.17, ALE-AME 0.13, ALE- PLE 0.16, PLE-PME 0.03, PME-PME 0.99. Eye area 1.20 long, 2.50 wide. Chelicerae chestnut, with 15 stout promargin teeth, 12 small retromarginal teeth, 10 tiny teeth with fang furrow (Fig. 11B). Labium and maxillae lightly yellow, with dark brown bristles and patch of cuspules on anterior half part (Fig. 11C); maxillae area length 3.49, cuspules area length 2.15. Sternum yellow, with three pairs of sigilla (Fig. 11D); palpal trochanter without lyral spines.

Leg spines. I: patella with 1 prolateral; tibia 2 ventral; metatarsus 6 ventral, and two comb-like organs near distad, ventral with 2 spines, prolateral with 2 spines (Fig. 12A-B); tarsus 6 prolateral, 5 retrolateral. II: patella 1 prolateral; tibia 1 prolateral, 2 ventral; metatarsus 1 prolateral, 7 ventral, and two comb-like organs near distad, retrolateral with 2 spines, prolateral with 2 spines (Fig. 12C-D); tarsus 5 prolateral spines, 5 retrolateral. III: patella 2 prolateral, 1 retrolateral; tibia 4 prolateral, 2 retrolateral, 4 ventral distally; metatarsus 3 dorsal, 5 prolateral, 3 retrolateral, 8 ventral, and two comb-like organs near distad, among them, retrolateral with 2 spines, prolateral with 2 spines (Fig. 12E-F); tarsus 8 prolateral, 7 retrolateral. IV: patella 1 prolateral near distally, 1 retrolateral; tibia 2 prolateral, 2 retrolateral, 2 ventral distal; metatarsus 2 dorsal, 4 prolateral, 5 retrolateral, 8 ventral, and two comb-like organs near distad, among them, retrolateral with 4 spines, prolateral with 2 spines (Fig. 12G-H); tarsus with 9 prolateral, 8 retrolateral.

Measurements of palp and legs: palp: 13.52 (4.54, 4.91, 4.07); I: 18.86 (5.46, 6.58, 4.19, 2.63); II: 21.53 (6.77, 7.25, 4.86, 2.65); III: 23.00 (6.55, 8.33, 5.13, 2.99); IV: 23.33 (5.78, 8.51, 6.28, 2.76). Leg formula: 4321.

Apically teardrop shaped receptacula bent inwards apically, the ratio of the length of the T to the length of the CD is almost 1 : 5 (Fig. 13). Spinnerets: PMS one segment, 1.94 long, 0.59 wide, PMS-PMS 1.05; PLS three segments, 12.00 long (3.33, 3.94, 4.73).

Males of Macrothele wuliangensis sp. n. resemble M. washanensis sp. n. by having similar palpal bulb morphology, but they can be distinguished by having spines in prolateral and dorsal views of palpal tibia and similar palpal bulb morphology, females of the new species are similar to others by the apically teardrop shaped receptacula bent inwards apically. Males of M. wuliangensis sp. nov. can be distinguished from M. washanensis sp. n. having five tibial spines visible in prolateral view, two tibial spines visible in dorsal view (Fig. 8G-I); tibia I has 10 ventral spines with six arranged in three pairs, tibia II has 7 ventral spines (Fig. 9) (vs four tibial spines visible in prolateral view, 0 dorsal spines; tibia I with nine spines visible in ventral view, tibia II has 3 ventral spines in M. washanensis sp. n.). Females of M. wuliangensis sp. n. can be differentiated from M. washanensis sp. n. by the ratio of the length of the T to the length of the CD is almost 1:5 (Fig. 13) (the ratio of the length of the T to the length of the CD is almost 1 : 6 in M. washanensis sp. n.).

The specific name refers to the type locality and is a noun in apposition.

China, Yunnan Province (Jingdong, Zhenyuan) (Fig. 15)

These spiders usually live in the gap between high rocks and soil (Fig. 14A). They use the natural gap to form a web (Fig. 14B). The web is obvious, funnel shaped, and there are many gaps (Fig. 14C). The spiders usually stay at the hole and wait for the appearance of prey. Generally, the female is large (Fig. 14D).

Male (holotype and two male paratypes, n = 3): total length 22.05-22.63; maxillary cuspules 210-330, labial cuspules 57-79. Female (paratypes, n = 16): total length 19.27-26.86; maxillary cuspules 219-354, labial cuspules 61-84.