Insect rearing and sample processing

DNA extraction

PCR amplification and PacBio sequencing of bacterial 16S rRNA gene

Statistical and bioinformatics analysis

Analysis of 16S rRNA sequencing results

Comparison of intestinal microbial diversity of Hyphantria cunea fed on different host plants

Functional prediction of gut microbiota

The composition of insect gut microbes is influenced by a variety of factors, with diet being the most critical

The gut microbes of H. cunea play important roles in nutrient acquisition, metabolism and pesticide degradation

The intestinal microbial composition of H. cunea feeding on different hosts was different and there were complex interactions amongst the different bacteria

The prediction results of intestinal microbial function in the plum feeding group were significantly different from those in the other feeding groups