Biodiversity Data Journal :
Taxonomy & Inventories
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Corresponding author: LI Qi-Rui (lqrnd2008@163.com)
Academic editor: Ning Jiang
Received: 08 Feb 2023 | Accepted: 17 Apr 2023 | Published: 15 May 2023
© 2023 Long Chun-Sheng, Wu You-Peng, Zhang Xu, Lin Yan, Shen Xiang-Chun, Ma Jian, LI Qi-Rui
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Chun-Sheng L, You-Peng W, Xu Z, Yan L, Xiang-Chun S, Jian M, Qi-Rui L (2023) Additions to hyphomycetes from Yungui Plateau, China with three new species (Ascomycota, Sordariomycetes). Biodiversity Data Journal 11: e101629. https://doi.org/10.3897/BDJ.11.e101629
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Yungui Plateau is rich in fungal diversity. Hyphomycetes, growing on submerged wood, can promote the degradation of organisms and the reuse of rotten wood energy. During an investigation of hyphomycetes in this region, 19 species of dematiaceous hyphomycetes were collected in Yungui Plateau.
Both morphological identification and multi-gene phylogenetic analyses of ITS, tef1 and LSU sequences supported Coryneum sevenseptatis as a new species. Phaeoisaria guizhouensis and Pleurothecium yunanensis were introduced, based on morphology. Morphological descriptions and illustrations of the new species were detailed. Known species are listed with notes.
Guizhou, karst area, new taxa, phylogenetic analysis, taxonomy
Coryneum firstly described by Nees based on the C. umbonatum Nees, 1816 (
Phaeoisaria was firstly described by
Pleurothecium was firstly described by
Yungui Plateau is a typical karst landform including Guizhou and Yunnan Provinces in south-western China (
Decaying wood with fungi were collected from forests in Guizhou and Yunnan Provinces. The samples were placed in paper bags and brought to the laboratory. Specimens were examined using an OLYMPUS SZ6 dissecting microscope. Photomicrographs were taken using a Cannon EOS 700D camera attached to a Nikon ECLIPSE Ni compound microscope. Measurements were made using the Tarosoft (R) Image Frame Work programme. Dimensions of anatomical features were shown followed
Strains used in the phylogenetic tree and their culture accession and GenBank numbers. Strains from this study are in bold.
SPECIES |
Strain number |
GenBank Accession number |
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ITS |
LSU |
tef |
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Asterosporium asterospermum |
KT2125 |
NA |
NA |
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Asterosporium asterospermum |
KT2138 |
NA |
NA |
||
Chaetoconis polygoni |
CBS 405.95 |
NA |
NA |
||
Coryneum castaneicola |
CFCC 52315 |
||||
Coryneum castaneicola |
CFCC 52316 |
||||
Coryneum depressum |
AR 3897 |
NA |
NA |
||
Coryneum gigasporum |
G14 |
||||
Coryneum gigasporum |
G15 |
||||
Coryneum heveanum |
MFLUCC 17-0369 |
NA |
|||
Coryneum heveanum |
MFLUCC 17-0376 |
||||
Coryneum ilicis |
CFCC 52994 |
NA |
|||
Coryneum ilicis |
CFCC 52995 |
NA |
|||
Coryneum modonia |
AR 3558 |
NA |
NA |
||
Coryneum perniciosum |
CBS 130.25 |
NA |
|||
Coryneum sinense |
X60 |
||||
Coryneum sinense |
X23 |
||||
Coryneum septemseptatum |
GMB0393 |
||||
Coryneum septemseptatum |
GMB0392 |
NA |
|||
Coryneum songshanense |
CFCC 52997 |
||||
Coryneum songshanense |
CFCC 52998 |
||||
Coryneum suttonii |
Z17 |
||||
Coryneum suttonii |
Z63 |
||||
Coryneum umbonatum |
CBS 199.68 |
NA |
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Coryneum umbonatum |
AR 3541 |
NA |
NA |
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Crinitospora pulchra |
CPC 22807 |
NA |
|||
Cytospora centrivillosa |
IT 2132 |
NA |
|||
Cytospora centrivillosa |
MFLU 17-0887 |
NA |
|||
Cytospora fertilis |
AR 3514 |
NA |
|||
Cytospora melanodiscus |
Jimslanding2 |
NA |
|||
Cytospora translucens |
CZ320 |
NA |
|||
Diaporthe azadirachtae |
TN 01 |
NA |
NA |
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Diaporthe eres |
AR 5193 |
||||
Diaporthe eres |
T-098 |
||||
Diaporthe maytenicola |
CPC 21896 |
NA |
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Hyaliappendispora galii |
MFLU 15-2269 |
||||
Lamproconium desmazieri |
MFLUCC 15-0870 |
||||
Lamproconium desmazieri |
MFLUCC 15-0872 |
||||
Macrohilum eucalypti |
CPC 10945 |
NA |
|||
Macrohilum eucalypti |
CPC 19421 |
NA |
|||
Pachytrype princeps |
Rogers s.n. |
NA |
NA |
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Pachytrype rimosa |
FF1066 |
NA |
NA |
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Phaeoacremonium aleophilum |
CBS 631.94 |
||||
Phaeoacremonium vibratile |
CBS 117115 |
||||
Phaeoappendicospora thailandensis |
TL 19 |
NA |
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Phaeoappendicospora thailandensis |
MFLU 12-2131 |
NA |
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Phaeodiaporthe appendiculata |
D77 |
NA |
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Prosopidicola mexicana |
CBS 113529 |
NA |
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Prosopidicola mexicana |
CBS 113530 |
NA |
NA |
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Rossmania ukurunduensis |
AR 3484 |
NA |
NA |
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Stegonsporium acerophilum |
D5 |
||||
Stegonsporium pyriforme |
D2 |
||||
Stilbospora macrosperma |
CBS 121883 |
||||
Stilbospora macrosperma |
WJ 1840 |
NA |
NA |
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Sydowiella depressula |
CBS 813.79 |
NA |
NA |
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Sydowiella fenestrans |
CBS 125530 |
The mycelia cultured on PDA medium were scraped out and put into a 1.5 ml centrifuge tube for DNA extraction. DNA Extraction Kit (E.Z.N.A.® Forensic DNA Kit, D3591, BIOMEGA, USA) was used for extraction of total DNA following its instructions. Three gene regions were amplified with universal primers. ITS1/ITS4 for the internal transcribed spacer regions of ribosomal DNA (ITS) (
The sequences were pasted into the BLASTN for preliminary identification. All sequences were selected, based on the top hits and the latest literature (
The Maximum Likelihood (RAxML) tree, based on a combined dataset of ITS, LSU and tef1 sequences. Bootstrap support values for Maximum Likelihood (ML, left) equal to or greater than 75% and Bayesian posterior probabilities (BY, right), equal to or greater than 0.95, are indicated at the nodes. When the value is less than 75/0.95, the value is represented by -/-. The tree is rooted to Phaeoacremonium vibratile (CBS 117115) and Phaeoacremonium aleophilum (CBS 631.94). The new collections are in bold. MH780882 (tef sequence of Coryneum heveanum) cannot be used in Phylogenetic analyses due to the abnormal Phylogenetic tree.
Saprobic on the surface of decaying wood (Fig.
With reference to the conidia with 7–8 septa.
Amongst the known species of Coryneum, C. betulinum Schulzer, C. gregoryi B. Sutton, C. japonicum (Sacc.) B. Sutton and C. psidii B. Sutton are similar to C. sevenseptatum in conidial shape (
Conidial sizes and numbers of distosepta of currently accepted Coryneum species.
Species name |
Conidia size (μm) |
No. of distosepta |
Reference |
Coryneum acaciae |
49-52 × 5-6 |
5-6 |
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Coryneum affine |
20-22 x 7 |
7 |
|
Coryneum arausiacum |
42–56 × 13–16 |
4–6 |
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Coryneum betulinum |
31–36 × 14–17 |
4–5 |
|
Coryneum berkeleyi |
30 x 8 |
3-5 |
|
Coryneum calophylli |
38–48 × 12.5–14.5 |
5–6 |
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Coryneum canadense |
45-75 x 13-15 |
3-5 |
|
Coryneum carpinicola |
50-68 x 8-11 |
7-11 |
|
Coryneum castaneicola |
56–80 × 9.5–13 |
5-8 |
|
Coryneum clusiae |
30–40 × 20–30 |
3-5 |
|
Coryneum compactum |
40–58 × 15–21 |
4-6 |
|
Coryneum cesatii |
80-90 x 13-15 |
6-7 |
|
Coryneum cocois |
40-42 x 3-4 |
2 |
|
Coryneum concolor |
10-11 x 4.5-5 |
3 |
|
Coryneum depressum |
44–53 × 19–23 |
4-6 |
|
Coryneum elevatum |
56-59 x 20-25 |
5-7 |
|
Coryneum eriobotryae |
5-9 x 5-7 |
1 |
|
Coryneum fagi |
45–75 × 10–15.5 |
6–12 |
|
Coryneum foliorum |
15-20 x 6-8 |
3 |
|
Coryneum gigasporum |
93–108 × 19–21 |
7-9 |
|
Coryneum gregoryi |
32.5–43 × 12–16 |
5-9 |
|
Coryneum heveanum |
43–53 x 15–20 |
4-6 |
|
Coryneum ilicis |
82-105 × 9.5-12.5 |
10–11 |
|
Coryneum japonicum |
45–60 × 11–12 |
5-7 |
|
Coryneum lanciforme |
45–53 × 16–18 |
4-6 |
|
Coryneum longistipitatum |
18-20 x 8-9 |
3 |
|
Coryneum megaspermum |
52-110 x 12-15 |
8-14 |
|
Coryneum megaspermum var. cylindricum |
100–125 × 10–13 |
7-8 |
|
Coryneum modonium |
50–71 × 14–19 |
5-8 |
|
Coryneum neesii |
68–82 × 18–22 |
6–8 |
|
Coryneum pruni |
14–23 × 5.5–9 |
4–5 |
|
Coryneum psidii |
25–40 × 14–17 |
5–6 |
|
Coryneum pyricola |
61–70 × 24–32 |
5–7 |
|
Coryneum septemseptatum |
34–46 × 14.5–17 |
7–8 |
This study |
Coryneum quercinum |
45–60 × 14–16 |
6–7 |
|
Coryneum sinense |
50–76 × 13–17 |
5–7 |
|
Coryneum songshanense |
51–76 × 9–11.5 |
5–7 |
|
Coryneum stromatoideum |
105–180 × 16–20 |
9–17 |
|
Coryneum suttonii |
60–76 × 10–14.5 |
4–5 |
|
Coryneum sydowianum |
50–58 × 14–17 |
5–6 |
|
Coryneum umbonatum |
57–72 × 13–16 |
5–7 |
|
Saprobic on the surface of decaying wood (Fig.
With reference to Guizhou Province where the type specimen was found.
Phaeoisaria guizhouensis morphologically resembles P. aquatica Z. L. Luo et al. and P. pseudoclematidis D.Q. Dai & K.D. Hyde, but they differ in conidial size (6.5–7.5 × 2.5–3.5 μm for P. aquatic; 9–13 × 1.9–3.6 μm for P. guizhouensis; 5–8.5 × 3–4 μm for P. pseudoclematidis) and the conidial of P. acquatica and P. pseudoclimatidis appears around the conidiophores, but P. guizouensis only appears at the apex (
Saprobic on the surface of decaying wood (Fig.
With reference to Yunnan Province where the type specimen was found.
Pleurothecium is characterisedby the distinct brown conidiophores and polyblastic, sympodially extended, densiculate conidiogenic cells (
Conidiophores 151–395 μm long (x̅ = 269.9 μm, SD = 65.7, n = 20), 3.6–6.5 μm wide (x̅ = 5 μm, SD = 1.0, n = 20), mononematous, erect, single, brown or dark brown, smooth. Conidiogenous cells 6–12 μm long (x̅ = 9.1 μm, SD = 2, n = 20), 2.5–3.5 μm wide (x̅ = 3 μm, SD = 0.5, n = 20) ampulliform to cylindrical, brown to dark brown, Conidia 15–20 μm long (x̅ = 17.3 μm, SD =1.4, n = 20), 7.9–12 μm wide (x̅ = 10 μm, SD = 1.2, n = 20), solitary, clavate, guttulate, truncated in base, hyaline, 3-septate, smooth-walled
Also see
Brachysporiella pulchra superficially resembles B. gayana Bat, but the conidia in B. pulchra are smaller (24–26 × 10.5–12.5 µm vs. 30–38 × 13–21.5 µm) (
Conidiophores 300–450 μm long (x̅ = 360.4 μm, SD = 30.8, n = 20), 3.6–6.5 μm wide (x̅ = 4.7 μm, SD = 0.92, n = 20), mononematous, branched in apex, erect, single, 5–7 septate, brown or dark brown, smooth. Conidiogenous cells lacking. Conidia 20–38 μm long (x̅ = 25.8 μm, SD =4.9, n = 20), 17–23 μm wide (x̅ = 18.4 μm, SD = 2.2, n = 20). Pyriform or obovoid brown or dark brown.
Also see
This species was originally assigned to Monotospora Corda by Berk and Curtis and later was transferred to Phragmocephala E.W. Mason & S. Hughes, Monosporella S. Hughes and Monotosporella S. Hughes (
Conidiophores 200–283 μm long (x̅ = 346.2 μm, SD =21.3, n = 20), 6–10 μm wide (x̅ = 7.5 μm, SD = 1.8, n = 20), cylindrical. Conidiogenous cells 21–40 μm long (x̅ = 28.5 μm, SD = 5.6, n = 20), 5.4–6.5 μm wide wide (x̅ = 6 μm, SD = 0.36, n = 20), monophialidic, integrated, terminal, cylindrical-clavate, with flared collarette. Conidia 13–15 μm long (x̅ = 14 μm, SD = 0.79, n = 20), 12–14 μm wide (x̅ = 13.2 μm, SD = 0.84, n = 20), formed in chains, aseptate, turbinate-triangular, with three blunt protruding edges at the broader distal end, hyaline to subhyaline when young, greyish-brown at maturity, smooth-walled.
Also see
Chaetosphaeria catenulata was firstly reported on submerged wood on the side of Nujiang River, Yunnan Province (
Conidiophores 190–346 μm long (x̅ = 294.1 μm, SD = 52.6, n = 20), 4.5–6.5 µm wide (x̅ = 5.5 μm, SD = 1.8, n = 20), mononematous, single, unbranched, septate, with 3-4 whorls of phialides in the mid-section and a single phialide at the apex, dark brown and paler towards the apex. Conidiogenous cells 10–14 μm long (x̅ = 12.5 μm, SD = 52.6, n = 20), 3–4 µm wide (x̅ = 5.5 μm, SD = 3.5, n = 20), cylindrical to lageniform, phialides, producing conidia from multiple entero-blastic conidiogenous loci and phialides borne on collar hyphae around the conidiophore. Conidia 3.5–4.5 μm long (x̅ = 4 μm, SD = 1.1, n = 20), 2.5–3.0 µm wide (x̅ = 2.5 μm, SD = 1.4, n = 20), globose to subglobose, aseptate and hyaline to subhyaline.
Also see
This species was originally collected on decaying wood in the Caribbean national forest and described as Melanopsammella gonytrichii F.A. Fernández & Huhndorf (
Conidiophores 3–5 μm long (x̅ = 2.8 μm, SD = 0.5, n = 20), 19.5–22.5 μm wide (x̅ = 3.6 μm, SD = 0.5, n = 20), micronematous, pale brown, smooth. Conidiogenous cells holoblastic, integrated, terminal, pale brown, cylindrical, smooth-walled. Conidia 49–55 μm long (x̅ = 52 μm, SD = 52.6, n = 20), 19.5–22.5 μm wide (x̅ = 21 μm, SD = 2.4, n = 20), solitary, acrogenous, cheiroid, pale brown to brown, consisting of 5–7 rows of cells, rows digitate, cylindrical, inwardly curved at the tip, arising from a basal cell euseptate, guttulate.
Also see
Dictyocheirospora rotunda, the type species of Dictyocheirospora, was collected on submerged wood in freshwater from Thailand (
Conidiophores 211–345.6 μm long (x̅ = 52.3 μm, SD = 52.3 n = 20), 4.4–9.5 µm wide (x̅ = 7.1 μm, SD = 2.1, n = 20), unbranched, erect, straight, attenuated, distinctly 5-8-septate, thick-walled, medium yellowish-brown, uniform in colour. Conidiogenous cells 120–280 μm long (x̅ = 199.1 μm, SD = 52.9, n = 20), 10–12 µm wide (x̅ = 10.8 μm, SD = 1.8, n = 20), integrated, polytretic with pores 0.8–1 μm diam., terminal and intercalary. Conidia 49–55 μm long (x̅ = 52 μm, SD = 5.2, n = 20), 19.5–22.5 μm wide (x̅ = 21 μm, SD = 3, n = 20), solitary, acrogenous, cheiroid, pale brown to brown, consisting of 5–7 rows of cells, rows digitate, cylindrical, inwardly curved at the tip, arising from a basal cell, without appendages, with each row composed of 8–12 cells, euseptate, guttulate, slightly constricted at septa.
Also see
Diplococcium dendrocalami was firstly introduced in the culms of Dendrocalamus sp. in the Philippines (
Conidiophores 37–66 μm long (x̅ = 52 μm, SD = 11.3, n = 20), 2.5–4.8 µm wide (x̅ = 3.1 μm, SD = 0.7, n = 20), macronematous, mononematous, single, unbranched, erect, straight or flexuous, septate, smooth, brown. Conidiogenous cells 3.6–5.6 μm long (x̅ = 4.4 μm, SD = 0.3, n = 20), 2.5–4.8 µm wide (x̅ = 3.6 μm, SD = 0.3, n = 20), monoblastic, integrated, terminal, cylindrical, smooth, brown to pale brown. Conidia 14.5–21 μm long (x̅ = 17.8 μm, SD = 3.3, n = 20), 6–7.5 µm wide (x̅ = 6.7 μm, SD = 0.5, n = 20), holoblastic, solitary, acrogenous, dry, clavate, smooth, lower two cells brown, apical cell pale brown, 2-septate.
Also see
Endophragmiella curvata has been found on dead branches from Guangdong Province, China (
Conidiophores 110–140 μm long (x̅ = 127.9 μm, SD =10, n = 20), 2.5–4.5 µm wide (x̅ = 2.9 μm, SD = 1.9, n = 20), macronematous, mononematous, single, unbranched, erect, straight or flexuous, septate, smooth, brown. Conidiogenous cells 12–13 μm long (x̅ = 12.4 μm, SD =0.34, n = 20), 3–4 µm wide (x̅ = 2.9 μm, SD = 0.4, n = 20), monoblastic, integrated, terminal, cylindrical, smooth, brown, percurrently proliferating. Conidia 12–17 μm long (x̅ = 14.1 μm, SD = 2.8, n = 20), 2–5 µm wide (x̅ = 3.4 μm, SD = 1.1, n = 20) holoblastic, solitary, acrogenous, dry, clavate, smooth, lower two cells brown, apical cell pale brown, 2–septate.
Also see
Conidiophores 200–350 μm long (x̅ = 280.6 μm, SD = 45, n = 20), 7–10 µm wide (x̅ = 8.6 μm, SD = 1.9, n = 20), mononematous, erect, simple, straight or flexuous, thick-walled, dark brown, paler towards the apex, 8–10 septate. Conidiogenous cells 48.5–60 μm long (x̅ = 53.5 μm, SD = 3.9, n = 20), 9–12 µm wide (x̅ = 10.2 μm, SD = 1.3, n = 20), with a narrow cytoplasmic channel and marked periclinal thickening in the upper quarter, lacking a collarette, proliferating enteroblastically to produce successive conidia at the same level. Conidia 21.5–40 μm long (x̅ = 29.3 μm, SD = 6.8, n = 20), 7.5–10 µm wide (x̅ = 8.2 μm, SD = 0.5, n = 20), holoblastic, solitary, accumulating in translucent slimy masses at the apices of conidiogenous, cylindrical, obtuse at the apex, slightly tapered towards the truncate base, hyaline, 3–euseptate, smooth, eguttulate.
Also see
Kylindria excentrica was firstly found on rotten wood in Ethiopia (
Conidiophores 3.5–4 µm diam. (x̅ = 3.6 μm, SD =1.2, n = 20), arising from a dark repent mycelium, more or less erect, dark brown, septate, irregularly branched, often forming a loop and network by anastomosing. Conidiogenous cells 1–1.5 µm long (x̅ = 1.3 μm, SD = 0.6, n = 20), 0.5–1 µm wide (x̅ = 0.8 μm, SD = 0.5, n = 20), holoblastic, monoblastic, integrated, intercalary or terminal, denticulate; denticles on the lower parts of conidiophores or directly arising on lateral of creeping fertile hyphae. Conidia diameter of coiled spores 12–15 µm (x̅ = 14.1 μm, SD =1.9, n = 20), pleurogenous, borne singly on minute hyaline sporogenous teeth, hyaline, tightly coiled 2½–3¼ times, indistinctly 18-20 septate.
Also see
Conidiophores 480–520 μm long (x̅ = 280.6 μm, SD =4 5, n = 20), 2–5 µm wide (x̅ = 3.7 μm, SD = 1.3, n = 20) macronematous, synnematous, erect, septate, smooth, mid-brown to dark brown. Conidiogenous cells 14.5–35.9 μm long (x̅ = 26.6 μm, SD = 4.4, n = 20), 1.6–3.8 μm wide (x̅ = 3.0 μm, SD = 0.6, n = 20) integrated, terminal, polyblastic, pale brown to hyaline, sympodial, splaying out with one to several denticulate conidiogenous cells loci. Conidia 3.5–5.5 μm long (x̅ = 4.5 μm, SD =1.1, n = 20), 2.5–4.8 µm wide (x̅ = 3.5 μm, SD =1.4, n = 20), globose to obovoid, hyaline, smooth-walled, guttulate, aseptate.
Also see
This species was originally discovered on decaying wood in Guizhou Province, China (
Conidiophores 128–157 μm long (x̄ = 142.5 µm, SD = 14.5, n = 20), 6.5–8.5 μm wide (x̄ = 7.5 µm, SD = 1, n = 20), synnematous, macronematous, septate, unbranched or branched, erect, dark brown at the base, pale brown at fertile, flared apex, sometimes proliferating, 5–8-septate. Conidiogenous cells 37–44 μm long (x̄ = 37.3 µm, SD = 2.1 µm, n = 20), 2.3–4 μm wide (x̄ = 2.5 µm, SD = 1.3 µm, n = 20), monoblastic, terminal, integrated, elongated, pale brown, often separating from the conidium through a break or frill below the base of conidium. Conidia 30–35 μm long (x̄ = 32.5 µm, SD = 2.5 µm, n = 20), 16–19 μm wide (x̄ = 17.5 µm, SD = 1.5 µm, n = 20), 4–septate, ellipsoidal to subglobose, dark brown, pale brown at apical and basal cells, with dark brown to black central cells, with a thick dark band on the central septum; smooth, rounded at apex, truncate at base, sometimes released with part of conidiogenous cell.
Also see
Phragmocephala atra is the type species of Phragmocephala, which is characterised by the dark brown to black central cells (
Conidiophores 97–120 μm long (x̄ = 99.7 µm, SD = 8.3, n = 20), 4.5–9 μm wide (x̄ = 6.2 µm, SD = 1.3, n = 20), straight or flexuous, septate, smooth, brown, with 3-4 branches at the apex. Conidiogenous cells 39–46 μm long (x̄ = 42.1 µm, SD = 4.0, n = 20), 7.6–12 μm wide (x̄ = 8.7 µm, SD = 2.4, n = 20) enteroblastic, phialidic, obscured by a shield of sterile cells. Conidia solitary, 1–septate, falcate, simple, smooth, hyaline, produced in slimy masses, 15.5–18 μm long (x̄ = 16.4 µm, SD = 1, n = 20), 1.2–1.4 μm wide (x̄ =1.3 µm, SD = 1.1, n = 20), solitary, 1–septate, falcate, simple, smooth, hyaline, produced in slimy masses.
Also see
Conidiophores 86–114 μm long (x̄ = 100 µm, SD = 5.3, n = 20), 5–7 μm wide (x̄ = 100 µm, SD = 5.3, n = 20) macronematous, mononematous, solitary, erect, unbranched, 7–9 septate, straight or flexuous, percurrently growing, dark brown, smooth. Conidiogenous cells 5–7 μm long (x̄ = 5.3 µm, SD = 2.2, n = 20), 4–5 μm long (x̄ = 4.8 µm, SD = 2.4, n = 20), monoblastic, integrated, terminal, dark brown. Conidia 45–63 μm long (x̄ = 54 µm, SD = 5.3, n = 20), 13–17 μm wide (x̄ = 15 µm, SD = 2.3, n = 20), acrogenous, solitary, ovoid to fusiform, 5–6-pseudoseptate, truncate at base, with a short and hyaline rostrate tip at apex, brown, smooth-walled.
Also see
Ellisembia brachypus was firstly reported as Sporidesmium branchypus in Shiwaliks (
Conidiophores 7.5–8.3 μm long (x̄ =7.9 µm, SD = 3.2, n = 20), 2.5–3 µm wide (x̄ = 2.6 µm, SD = 2, n = 20), micronematous, hypha-like, cylindrical, aseptate, simple or sparsely branched, smooth, hyaline. Conidiogenous cells 20–40 μm long (x̄ =34.2 µm, SD = 4.3, n = 20), 4–6 µm wide (x̄ = 5.3 µm, SD = 2.3, n = 20), hyaline, clavate to vermiform. Conidia 17–23 μm long (x̄ = 20.4 µm, SD = 4.1, n = 20), 8–13 µm wide (x̄ = 11.3 µm, SD = 3.3, n = 20), acrogenous, solitary, clavate to obpyriform, smooth, brown to dark brown, aseptate.
Also see
The genus Vanakripa was originally established by
Conidiophores 37–50 μm long (x̄ = 42.2 µm, SD = 4.6, n = 20), 5–6.8 µm wide (x̄ = 5.9 µm, SD = 0.6, n = 20), macronematous, mononematous, solitary, erect, unbranched, 1–3 septate. Conidiogenous cells 10–12 μm long (x̄ =10.8 µm, SD =0.5, n = 20), 4-5 μm long (x̄ = 4.8 µm, SD = 2.4, n = 20), monoblastic, integrated, terminal, dark brown. Conidia 36–52 μm long (x̄ = 36.6 µm, SD = 12.7, n = 20), 8–10 μm wide (x̄ = 9.4 µm, SD = 2.5, n = 20), acrogenous, solitary, clavate to broadly fusiform, 5–6 septate, with a short and hyaline rostrate tip at apex, brown, smooth-walled.
Also see
Many genera of hyphomycetes were found in Karst areas, such as Acrogenospora M.B. Ellis, Craspedodidymum Hol.-Jech, Corynesporopsis P.M. Kirk, Dactylella Grove, Dendryphiopsis S. Hughes, Digitoramispora R.F. Castañeda & W.B. Kendr., Diplocladiella G. Arnaud, Endophragmiella B. Sutton, Elegantimyces Goh, C.K.M. Tsui & K.D. Hyde, Exosporium Link, Gangliostilbe Subram. & Vittal, Helminthosporium Link, Heteroconium Petr., Microclava F. Stevens, Monodictys S. Hughes, Mucispora Jing Yang, Bhat & K.D.Hyde, Phalangispora Nawawi & J. Webster, Phragmocephala E.W. Mason & S. Hughes, Repetophragma Subram., Spadicoides S. Hughes, Sympodioplanus R.C. Sinclair & Boshoff, Synnemacrodictys W.A. Baker & Morgan-Jones, Tretospeira Piroz. and Ulocladium Preuss (
After consulting the relevant literature and our experimental experience, the authors found that numerous hyphomycetes occurring on wood cannot be cultured which led to lack of DNA sequences, resulting in confusion in identification and classification (
This research was supported by the National Natural Science Foundation of China (32000009 and 31960005); the Fund of the Science and Technology Foundation of Guizhou Province ([2020]1Y059); Guizhou Province Ordinary Colleges and Universities Youth Science and Technology Talent Growth Project [2021]154.