Biodiversity Data Journal :
Taxonomy & Inventories
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Corresponding author: Cuong The Pham (cuongiebr@gmail.com), Anh Van Pham (phamanh@hus.edu.vn)
Academic editor: Bin Wang
Received: 06 Jan 2025 | Accepted: 09 Apr 2025 | Published: 30 Apr 2025
© 2025 Vien Nguyen, Tien Phan, Dang Do, Truong Nguyen, Thomas Ziegler, Cuong Pham, Anh Pham
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Nguyen VT, Phan T, Do D, Nguyen T, Ziegler T, Pham C, Pham A (2025) New provincial records of the genus Limnonectes (Amphibia, Anura, Dicroglossidae) from South Central, Vietnam. Biodiversity Data Journal 13: e146098. https://doi.org/10.3897/BDJ.13.e146098
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Limnonectes is one of the poorly-known genera of amphibians in Vietnam. In the herpetofaunal list of Vietnam in 2009, only five species of the genus Limnonectes were recorded from the country. Recently, four new species (L. nguyenorum, L. quangninhensis, L. kiziriani and L. phuyenensis) and three new country records (L. gyldenstolpei, L. fastigatus and L. kohchangae) were reported from Vietnam.
Based on recent field-work in South Central of Vietnam, we report four new provincial records of the genus Limnonectes from Phu Yen, Khanh Hoa, Binh Thuan and Gia Lai Provinces, namely L. dabanus, L. fastigatus, L. limborgi and L. phuyenensis. In addition, morphological data and ecological notes of the aforementioned species are provided.
distribution, Limnonectes, new records, morphology, taxonomy
The genus Limnonectes Fitzinger, 1843 currently contains 93 recognised species with a wide distribution in Asia, from eastern and southern China, eastwards to Japan, throughout Indochina and southwards to Malaysia, Indonesia, the Philippines and New Guinea (
Limnonectes dabanus (Smith) and L. limborgi (Sclater) are listed as Least Concern in the IUCN Red List (
Based on our recent fieldwork in Vietnam between 2022 and 2024, we herein report four new provincial records of the genus Limnonectes from Vietnam: L. dabanus from Binh Thuan Province, L. fastigtus from Gia Lai Province, L. limborgi from Khanh Hoa and Phu Yen Provinces and L. phuyenensis from Khanh Hoa Province.
Sampling
Field surveys were conducted in three localities in South Central Vietnam: in Takou Nature Reserve, Binh Thuan Province in March 2022; in Van Ninh District, Khanh Hoa Province in May 2023 and in October 2024; in Son Hoa District, Phu Yen Province in October 2024; and in Kon Cha Rang Nature Reserve, Gia Lai Province in May 2024.
Frogs were collected by hand between 19:00 and 22:00 h. After taking photographs, frogs were anaesthetised and euthanised in a closed vessel with a piece of cotton wool containing ethyl acetate (
Morphological characters
Measurements were taken with a digital caliper to the nearest 0.1 mm. Morphological terminology followed
Molecular analysis
The tissue samples of Limnonectes were extracted using QIAamp DNA Mini Kit (Qiagen, Germany) following protocols by the manufacturer. Total DNA was then amplified by HotStar Taq Mastermix (Qiagen, Germany). The standard PCR conditions were 95oC for 15 min to active Taq; 35 cycles at 95oC for 30s, 50oC for 45s, 72oC for 1 min; and a final elongation at 72oC for 10 min. The PCR volume contained 2 µl of each primer at 10 µmol/µl, 5 µl water, 10 µl of Mastermix and 2 µl DNA template. The primers used to amplify a fragment of the mitochondrial DNA 16S gene were AR (5’-CGCCTGTTTATCAAAAACAT-3’) and BR (5’-CCGGTCTGAACTCAGATCACGT-3’) (
One sequence of 560 bps (16S gene) from specimen IEBR A.6338 (GenBank accession number PV444292) of Limnonectes specimen from Ta Kou Nature Reserve, Binh Thuan Province was similar (99.5%) to the available sequence of L. dabanus (accession number MK688610) on GenBank.
Morphological characters of specimens from Binh Thuan Province agreed with the descriptions of
Measurements (in mm) of the specimens of Limnonectes from South Central Vietnam (abbreviations defined in the text).
Limnonectes dabanus |
Limnonectes fastigatus |
Limnonectes limborgi |
Limnonectes phuyenensis |
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Male (n=2) |
Females (n=2) |
Male (n=2) |
Male (n=1) |
Female (n=3) |
Male (n=4) |
Female (n=1) |
|
SVL |
52.4-53.3 |
44.7-44.9 |
58.9-68.6 |
36.4 |
33-34.5 |
61.5-69.9 |
45.8 |
HL |
23.5-27.3 |
18.2-18.3 |
26.3-29.8 |
15.5 |
12.5-14.2 |
28.5-32.2 |
19.9 |
HW |
23.8-27.6 |
18.3-18.5 |
27.1-32.6 |
15.0 |
12.3-13.7 |
29.7-33.3 |
20.5 |
RL |
8.5-9.5 |
6.8-7.1 |
8.5-10.2 |
6.0 |
5.0-5.3 |
8.6-9.9 |
7.0 |
ED |
5.7-5.9 |
5.1-5.2 |
7.1-8.0 |
4.0 |
4.3-4.5 |
7.2-8.4 |
5.9 |
UEW |
2.9-3.5 |
3.1-3.2 |
3.6-3.8 |
2.6 |
2.5-3.3 |
3.5-4.3 |
2.8 |
IND |
5.3-5.9 |
4.3-4.5 |
6.0-6.2 |
4.6 |
4.0-4.5 |
5.5-6.3 |
4.9 |
IOD |
4.6-4.8 |
3.5-3.7 |
5.6-5.8 |
3.6 |
3.0-3.2 |
5.4-5.8 |
3.7 |
NS |
3.4-4.4 |
2.8-3.1 |
4.1-4.8 |
3.1 |
2.3-2.6 |
3.4-4.8 |
3.0 |
EN |
4.9-5.3 |
3.8-3.9 |
4.4-5.4 |
2.9 |
2.8-2.9 |
4.4-5.0 |
3.9 |
TD |
5.2-5.3 |
3.4-3.5 |
NA |
2.1 |
2.7-2.8 |
NA |
NA |
TYE |
4.0-4.4 |
2.6-2.8 |
NA |
2.2 |
1.5-1.8 |
NA |
NA |
UAL |
10.6-12.3 |
9.1-9.6 |
11.8-14.3 |
7.3 |
7.1-7.4 |
11.0-12.6 |
8.6 |
FAL |
22.1-22.2 |
19.8-20 |
23.1-29.1 |
14.6 |
12.0-13.6 |
25.1-29.2 |
20.5 |
FeL |
23.9-24.2 |
21.1-21.7 |
27.2-31.1 |
18.8 |
16.5-17.4 |
29.2-31.7 |
23.2 |
TbL |
26.3-26.5 |
22.0-22.8 |
26.1-29.8 |
19.2 |
17.6-18.0 |
27.1-29.3 |
21.5 |
TbW |
8.1-8.3 |
6.5-6.9 |
10.8-12.8 |
5.4 |
4.2-4.8 |
13.0-14.2 |
9.2 |
FoL |
25.4-25.5 |
21.6-22.9 |
32.5-38.6 |
27.7 |
24.5-25.1 |
36.0-37.5 |
28.7 |
HL/SVL |
0.45-0.51 |
0.41-0.41 |
0.43-0.45 |
0.43 |
0.37-0.41 |
0.45-0.48 |
0.43 |
HW/SVL |
0.44-0.48 |
0.4-0.41 |
0.46-0.48 |
0.41 |
0.36-0.40 |
0.47-0.51 |
0.45 |
RL/SVL |
0.16-0.18 |
0.15-0.16 |
0.14-0.15 |
0.16 |
0.15-0.16 |
0.14-0.15 |
0.15 |
HL/HW |
1.02-1.06 |
1.01-1.03 |
0.91-0.97 |
1.03 |
1.02-1.06 |
0.94-0.97 |
0.97 |
ED/RL |
0.60-0.68 |
0.73-0.75 |
0.78-0.84 |
0.67 |
0.83-0.9 |
0.75-0.93 |
0.84 |
TYE/TD |
0.70-0.74 |
0.74-0.82 |
NA |
1.05 |
0.56-0.67 |
NA |
NA |
TD/ED |
0.90-0.91 |
0.85-0.88 |
NA |
0.53 |
0.6-0.63 |
NA |
NA |
TbL/SVL |
0.49-0.51 |
0.49-0.51 |
0.43-0.44 |
0.53 |
0.52-0.53 |
0.43-0.48 |
0.49 |
TbL/TbW |
3.17-3.27 |
3.30-3.38 |
2.33-2.42 |
3.56 |
3.67-4.26 |
2.10-2.26 |
2.46 |
Arms short; fingers free of webbing; tips of fingers blunt, not expanded; finger I with nuptial pad, without minute spines in males. Tibia length shorter than thigh; tips of toes blunt, round; webbing well developed, formula I0–1/3II0–1/4III0–1IV1–0V; tibio-tarsal articulation reaching to the eye (Fig.
Skin texture in life: Dorsal surface of head smooth with a swollen flap in males; dorsal surface of body and flanks with tubercles; supratympanic fold distinct; dorsal surface of limbs and thighs smooth; dorsal surface of tibia with small tubercles; throat, chest, belly and ventral surface of thighs smooth.
Colouration in life: Dorsal surface of head, body and flanks light brown with dark brown marking; dorsal surface of fore- and hind-limbs light brown with dark crossbars; ventral surface of limbs, throat, chest and belly white (Fig.
In Vietnam, this species has been reported from Dak Lak, Dak Nong, Lam Dong, Dong Nai, Binh Dinh, Phu Yen, Ninh Thuan and Khanh Hoa Provinces (
The frogs were found between 21:00 and 22:30 h in rocky streams. Surrounding habitat consisted of mixed secondary forest composed of small to medium hardwoods and shrubs. Air temperature was 25–30°C and relative humidity was 55–70%.
One sequence of 560 bps (16S gene) from specimen IEBR A.6341 (GenBank accession number PV444293) of Limnonectes specimen from Kon Cha Rang Nature Reserve, Gia Lai Province was similar (98.2%) to the available sequence of L. fastigatus (accession number MT459155) on GenBank.
Morphological characters of specimens from Gia Lai Province agreed with the descriptions of
Arms short; upper arm length (UAL 11.8-14.3 mm in males), forearm length (FAL 23.1-29.1 mm in males); fingers free of webbing; dermal ridge on sides of fingers II and III; tips of fingers blunt, not expanded; inner metatarsal tubercle large, oval; outer metatarsal tubercle small, elongate; finger I of males with nuptial pad, composed of minute spines on the dorsal surface and medial edge (Fig.
Tibia length shorter than thigh length (FeL 27.2-31.1 mm, TbL 26.1-29.8 mm in males), longer than wide (TbL/TbW 2.33-2.42 in males); tips of toes blunt, slightly round; toes webbed to the middle of terminal phalanx at base of toe pad; webbing well developed; dermal ridge on outer sides of toes I and V; inner metatarsal tubercle elongate; outer metatarsal tubercle absent; tibio-tarsal articulation reaching behind the eye (Fig.
Skin texture in life. Dorsal surface of head and body crenulate; small tubercles on the upper eyelid; flanks, around cloaca and dorsal surface of limbs and thighs with small tubercles; supratympanic fold distinct, extending from eye to angle of the jaw; dorsolateral fold absent; dorsal surface of tibia and foot distinctly tuberculate, covered with moderately dense, small, tubercles; throat, chest, belly and ventral surface of thighs smooth.
Colouration in life. Iris light brass with dark grey-brown lines bisecting eye vertically and horizontally through pupil; head with a pale yellow bar in the anterior interorbital region and a narrow dark brown bar in the posterior interorbital region; dorsum and flanks with dark grey-brown marking; lips with dark bars; supratympanic fold black; dorsal surface of limbs irregular, yellowish-brown with dark crossbars; ventral surface of limbs, throat and chest white with brown markings; belly immaculate white; toe webbing brown; the tip of fingers and toes white (Fig.
In Vietnam, this species was recorded in Kon Tum Province (
The frogs were found between 19:00 and 23:00 h in the water of rocky streams or on the ground. The surrounding habitat was a mixed secondary evergreen forest of the large, medium and small hardwoods and shrubs. Air temperature was 18–28°C and relative humidity was 76–86%.
One sequence of 560 bps (16S gene) from specimen IEBR A.6343 (GenBank accession number PV444294) of Limnonectes specimen from Van Ninh District, Khanh Hoa Province was similar (100%) to the available sequence of L. limborgi (accession number GU934345) on GenBank.
Morphological characters of specimens from Phu Yen and Khanh Hoa Provinces agreed with the descriptions of
Arms short and thin; fingers free of webbing; tips of fingers blunt, not expanded into discs; nuptial pad absent on finger I in males. Tibia and thigh short; tips of toes blunt, not expanded into discs; webbing formula I1–2II11/2–21/2III2–3IV31/2–11/2V; tibio-tarsal articulation reaching between eye and tip of snout (Fig.
Skin texture in life: Dorsal surface of head and body with small tubercles; supratympanic fold distinct; dorsolateral folds present; ventral surface smooth.
Colouration in life: Dorsal surface light brown or light yellow with small dark spots; a dark cross bar between the eyes and a ᴧ-shaped mark between the shoulders; thighs and tibia with dark brown cross bars; ventral surface cream; throat cream with dark pattern (Fig.
In Vietnam, this species is known from Lao Cai in the north southwards to Dong Nai and Kien Giang Provinces (
The frogs were found between 19:00 and 22:30 h on forest paths. Surrounding habitat consisted of mixed secondary forest composed of small to medium hardwoods and shrubs. Air temperature was 23–30°C and relative humidity was 65–81%.
One sequence of 560 bps (16S gene) from specimen IEBR A.6348 (GenBank accession number PV444294) of Limnonectes specimen from Son Hoa District, Phu Yen Province was similar (99.8%) to the available sequence of L. phuyenensis (accession number MW222163) on GenBank.
Morphological characters of specimens from Khanh Hoa Province agreed with the description of
Arms short, finger free of webbing, tips of fingers blunt, not expanded, fingers I with nuptial pad, composed of minute spines on dorsal surface and medial edge in males. Tibia shorter than thigh (FeL 29.2-31.7 mm, TbL 27.1-29.3 mm in males; 23.2 mm, 21.5 mm in female); tips of toes blunt, not expanded into discs toes webbed to distal of terminal phalanx; webbing well developed; tibio-tarsal articulation reaching to the tip of snout (Fig.
Skin texture in life. Dorsal surface of head, body and flanks with ridges and tubercles; dorsal surface of tibia and foot distinctly tuberculate, covered with moderately dense, small, low tubercles; supratympanic fold present; dorsolateral fold absent.
Colouration in life. Dorsum yellowish-brown with dark brown markings; ventral surface white with brown markings (Fig.
In Vietnam, this species was previously known only from Phu Yen Province (
The frogs were found between 19:00 and 22:30 h on the stones, on the ground or in the water of rocky streams. Surrounding habitat consisted of mixed secondary forest composed of large to medium hardwoods and shrubs. Air temperature was 22–29°C and relative humidity was 72–88%.
In terms of distribution, members of the genus Limnonectes are found in different geographical units in the Indochina Region (see
List of genus Limnonectes recorded from Vietnam. RBVN (2024) = Vietnam Red Data Book VU = Vulnerable (
Name |
RBVN 2024 |
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Distribution by subregions of Indochina ( |
Limnonectes bannaensis Ye, Fei, Xie & Jiang, 2007 |
NWU, NEU, NEL, NAN |
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Limnonectes dabanus (Smith, 1922) |
CAN, CSL, SAN |
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Limnonectes fastigatus Stuart, Schoen, Nelson, Maher, Neang, Rowley & McLeod, 2020 |
CAN |
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Limnonectes gyldenstolpei (Andersson, 1916) |
NAN, UML, CMB |
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Limnonectes kiziriani Pham, Le, Ngo, Ziegler & Nguyen, 2018 |
CAN |
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Limnonectes kohchangae (Smith, 1922) |
CBM, MEK |
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Limnonectes limborgi (Sclater, 1892) |
NWU, NEU, NEL, NAN, UML, SLU, SLL, CBM, CAN, CSL, SAN, MEK |
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Limnonectes nguyenorum McLeod, Kurlbaum & Hoang, 2015 |
NT |
NWU, NEL, NEU |
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Limnonectes phuyenensis Pham, Do, Le, Ngo, Nguyen, Ziegler & Nguyen, 2020 |
CAN |
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Limnonectes poilani (Bourret, 1942) |
CAN, CBM, SAN, CSL |
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Limnonectes quangninhensis Pham, Le, Nguyen, Ziegler, Wu & Nguyen, 2017 |
VU |
VU |
NEU, NIS |
In terms of taxonomy, several populations of the L. bannaensis complex in Vietnam were described as new species, viz. L. nguyenorum from Ha Giang Province (
The Annam Wart Frog (Limnonectes dabanus) was described from Lam Dong Province, Vietnam by
In terms of conservation concern, one is listed as Vulnerable (VU) in the Red Data Book of Vietnam (
We are grateful to the Directorates of the Forest Protection Department of Phu Yen, Khanh Hoa, Binh Thuan, Gia Lai Provinces and Kon Cha Rang, Ta Kou Nature Reserves for supporting our fieldwork. We thank CV Hoang and LHT Le (Hanoi) for their assistance in the field. For the productive collaboration within joint research projects, we cordially thank AH Le (IEBR, Hanoi), as well as T Pagel and C Landsberg (Cologne Zoo). Fieldwork in Vietnam was supported by the Cologne Zoo.