Biodiversity Data Journal : Taxonomic Paper
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Taxonomic Paper
A preliminary checklist of soil ants (Hymenoptera: Formicidae) of Colombian Amazon
expand article infoDaniel Castro, Fernando Fernández§, Andrés D Meneses§, Maria C Tocora§, Stepfania Sanchez§, Clara P Peña-Venegas
‡ Instituto Amazónico de Investigaciones Científicas SINCHI, Leticia, Colombia
§ Instituto de Ciencias Naturales, Universidad Nacional de Colombia, Bogotá, Colombia
Open Access

Abstract

Background

This paper presents an updated list of soil ants of the Colombian Amazon collected in three different river basins: the Amazon, the Caquetá and the Putumayo. The list includes 10 subfamilies, 60 genera and 218 species collected from TSBF monoliths at four different depths (Litter, 0 - 10 cm, 10 - 20 cm and 20 - 30 cm). This updated list increases considerably the knowledge of edaphic macrofauna of the region, due to the limited published information about soil ant diversity in the Colombian Amazon region.

New information

This is the first checklist of soil ant diversity of the Colombian Amazon region. Six new records of species for Colombia are exposed: Acropyga tricuspis (LaPolla, 2004), Typhlomyrmex clavicornis (Emery, 1906), Typhlomyrmex meire (Lacau, Villemant & Delabie, 2004), Cyphomyrmex bicornis (Forel, 1895), Megalomyrmex emeryi (Forel, 1904) and Myrmicocrypta spinosa (Weber, 1937), most of them corresponding to subterranean ants.

Keywords

TSBF, Amazon basin, soil macrofauna, biogeography, species distribution.

Introduction

In tropical forests, the abundance and diversity of ants is usually high, which brings out the importance of ants for these ecosystems (Floren and Linsenmair 2005, Floren et al. 2002, Dunn et al. 2007, Davidson et al. 2007, Jaffe et al. 2007). Ants, together with earthworms and termites, are known as "ecosystem engineers" due to the positive effect of their activity on ecosystems (Decaëns et al. 1999, Decaëns 2010, Lavelle et al. 1997, Luke et al. 2014, Griffiths et al. 2017). Physical, chemical and biological soil properties are positively affected by the presence of ant nests, chambers, galleries and mineral aggregates that ants create (Seybold et al. 1999, Barros et al. 2001, Sanabria et al. 2014, Wu et al. 2015)

Diversity of soil ants in Amazonian forests is notoriously high (Ryder Wilkie et al. 2010, Ryder Wilkie et al. 2007, Bastos and Harada 2011, Bruna et al. 2008). In Colombia, ant lists include reports from coastal, mountain and Amazonian ecosystems (Pérez et al. 2009, Sanabria-Blandón and Achury 2011, Sanabria-Blandón and de Ulloa 2011, Vergara-Navarro and Serna 2013, Valdés-Rodríguez et al. 2014). However, information on soil ant diversity in the Colombian Amazon region is limited, due to the small number of works on this topic that have been published (Ospina and Fagua 2007).

This paper reports a preliminary checklist of soil ants collected in the Colombian Amazon region, with the purpose of contributing to a better understanding of the biogeographical distribution of these insects in the three most important river basins of the Amazon region of Colombia: the Amazon, the Caquetá and the Putumayo.

Materials and methods

Study area

Three Colombian states of the Amazon region were sampled: Amazonas, Caquetá and Putumayo (Fig. 1). The study area includes the Andean-Amazonian transition from north to south of the Colombian Amazon region up to the borders with Peru and Brazil along the Amazon River. Sampling altitude went from 78 to 2275 metres above sea level. There, 71 sampling sites in 13 municipalities were sampled: in Caquetá, the municipalities of Belén de los Andaquíes, Florencia, Morelia, San José and Solano; in Putumayo, the municipalities of Puerto Leguizamo and La Tagua; in Amazonas, the municipalities of El Encanto, La Chorrera, Leticia, Puerto Alegria, Puerto Arica, Puerto Nariño and Puerto Santander. Different natural and anthropic land uses were included in the sampling: primary and secondary forests, young secondary forests, pastures and indigenous slash-and-burn agricultural plots (Table 1).

List of TSBF monoliths sampling sites in Colombian Amazon soils.

River basin State Town Land use Altitude Latitude Longitude
Amazonas Amazonas Leticia Primary forest 80 S4°10'09", W69°57'25"
Primary forest 81 S4°10'09.1", W69°57'27.2"
Primary forest 98 S04°07'15.4", W69°57'19.7"
Primary forest 106 S04°02'45.7", W69°59'26.8"
Primary forest 110 S04°00'32.5", W69°53'43.3"
Primary forest 119 S04°00'10.5", W69°53'47.6"
Primary forest 121 S04°02'48.0", W70°00'20.4"
Secondary forest 87 S04°07'14.7", W69°56'40.9"
Pto. Nariño Secondary forest 84 S03°46'33.6", W70°21'41.8"
Young secondary forest 102 S03°46'52.6", W70°21'17.7"
Caquetá Pto. Santander Secondary forest 116 S00°39'43.3", W72°18'38.2"
Caquetá Belen Pasture 233 N01°16ˈ08.3", W75°47ˈ17.6"
Pasture 242 N01°15ˈ59.9", W75°47ˈ23.4"
Primary forest 500 N01°36'17.8", W75°52'50.9"
Primary forest 625 N01°36'28.6", W75°53'12.6"
Primary forest 750 N01°37'50.3", W75°54'21.3"
Primary forest 875 N01°37'40.0", W75°54'16.8"
Primary forest 1000 N01°37'27.4", W75°54'04.3"
Primary forest 1125 N01°40'14.4", W75°54'13.3"
Primary forest 1247 N01°50'36.4", W75°40'18.3"
Primary forest 1250 N01°40'45.2", W75°54'12.4"
Primary forest 1375 N01°40'54.3", W75°54'17.1"
Primary forest 1500 N01°42'06.8", W75°53'57.5"
Primary forest 1625 N01°41'49.9", W75°54'18.1"
Primary forest 1875 N01°43'04.4", W75°54'11.7"
Young secondary forest 250 N01°25'46.2", W75°45'01.01"
Young secondary forest 251 N01°25'57,6", W75°45'06,4"
Young secondary forest 268 N01°26'45.7", W75°48'12.4"
Young secondary forest 271 N01°25'42,7", W75°46'56,3"
Florencia Pasture 383 N01°38ˈ54,1", W75°38ˈ13,6"
Pasture 437 N01°39'15.6", W75°38'00.2"
Pasture 527 N01°43'00.12", W75°36'49,3"
Pasture 2275 N01°45'33.7", W75°46'41.5"
Pasture 981 N01°42'29.8", W75°41'32.4"
Pasture 1268 N01°42'55.1", W75°42'06.0"
Secondary forest 259 N01°26ˈ39.9", W75°31ˈ29.1"
Secondary forest 488 N01°40'35.0", W75°37'5.86"
Secondary forest 495 N01°42'26.8", W75°36'59.5"
Secondary forest 598 N01°43'04.0", W75°36'45.6"
Secondary forest 1328 N01°42'37.7", W75°43'49.1"
Secondary forest 1571 N01°50ˈ09,0", W75°40ˈ19,2"
Young secondary forest 241 N01°42'52.2", W75°36'53.6"
Young secondary forest 246 N01°42'27.6", W75°43'26.0"
Young secondary forest 260 N01°26ˈ40.9", W75°31ˈ32.1"
Young secondary forest 425 N01°40'47.0", W75°37'48.3"
Young secondary forest 506 N01°42'27.9", W75°36'59.7"
Young secondary forest 1617 N01°50'36.9", W75°40'16.1"
Morelia Pasture 252 N01°27'21.63", W75°39'48.10"
Secondary forest 249 N01°26ˈ28.8", W75°39ˈ10.3"
Young secondary forest 261 N01°26'18.1'", W75°45'16.3"
Young secondary forest 555 N01°26ˈ29.9", W75°39ˈ12.5"
Young secondary forest 248 N01°39'35.2", W75°36'33.9"
San José Primary forest 284 N01°11ˈ38.4", W75°58ˈ16.7"
Young secondary forest 288 N01°11ˈ40.1", W75°58ˈ18.7"
Solano Young secondary forest 106 S00°34'30.8", W72°06'51"
Putumayo Amazonas El Encanto Primary forest 140 S01°37'03.7", W73°15'31.7"
Primary forest 141 S01°40'34.7", W73°13'51.4"
La Chorrera Primary forest 126 S02°04'55.2", W72°10'54.8"
Primary forest 133 S02°04'14.4", W72°10'14.2"
Primary forest 146 S01°26'54.2", W72°48'13.3"
Primary forest 151 S01°26'56.3", W72°48'37"
Primary forest 154 S01°25'05.7, W72°47'21.2"
Secondary forest 147 S01°25'11", W72°47'10.5"
Pto. Alegria Primary forest 154 S01°00'31.5", W74°04'44.5"
Primary forest 169 S00°59'34.3", W74°01'10.4"
Pto. Arica Primary forest 108 S02°07'55.6", W71°44'42.8"
Primary forest 120 S02°07'59", W71°46'54"
Primary forest 127 S02°08'10.5", W71°43'16.8"
Sabalo Primary forest 142 S02°21'11.7", W72°35'53.4"
Putumayo Pto. Leguizamo Secondary forest 182 S00°05'14.9", W74°36'38.4"
Secondary forest 213 S00°08'42.1", W74°46'40.9"
Figure 1.  

Study area, sampling localities.

Sample collection and analysis

Soil ant collection took place between September 2015 and July 2017. Soil ants were collected using the methodology suggested by the Tropical Soil Biology and Fertility Program (TSBF) for soil macrofauna collection (Anderson and Ingram 1993). In each sampling site, a plot of 60 x 60 metres was selected. There, five monoliths of 25 x 25 x 30 cm of depth were done: one in each corner of the 60 x 60 m delimited square plot and one in the centre of it. In each monolith, macrofauna samples were collected at four depths: litter, 0 – 10 cm, 10 – 20 cm and 20 – 30 cm. Macrofauna collection in each monolith depth was undertaken in the field manually. Recovered samples were preserved in ethanol at 75% until their arrival to the SINCHI Institute laboratories in Leticia, Colombia, where specimens were vouchered and preserved in the CATAC collection.

In the laboratory, samples were cleaned and classified into morphotypes and species. All samples were identified by using the keys of recent revisions, verifying the species with the diagnosis and in some cases comparing with photos of type material in AntWeb (Brandão 1990, Kugler 1994, De Andrade and Baroni 1999, Palacio 1999, Fernández 2003, Longino and Fernández 2007, Jiménez et al. 2008, Mackay and Mackay 2010, Ortiz and Fernández 2011, Pacheco and Mackay 2013, Lenhart et al. 2013, Ješovnik and Schultz 2017, AntWeb 2018, LaPolla 2004, Snelling and Longino 1992, Brandão 2003, Longino 2010, Fernández et al. 2015, Lattke et al. 2007, Lattke 1997, Longino 2013, Sosa-Calvo et al. 2018, Longino 2003). Camponotus, Brachymyrmex and Pheidole were identified through the comparison of material identified by specialists and reference collection. All data were organised alphabetically by subfamily, genus and species in an ant checklist following the nomenclature suggested in the Bolton online catalogue of the ants of the world (AntCat, Bolton 2018).

Analysis

Checklist of the soil ant species of Colombian Amazon

A total of 1341 specimens and 4318 individuals were analysed. From the total soil macrofauna, ants were the most abundant and species-richest organisms collected. Ants dominated litter and 0 - 10 cm depths (Barros et al. 2002, Mathieu et al. 2005, Rossi et al. 2006, Velásquez et al. 2012, Suárez Salazar et al. 2015). Litter had the highest species richness with 129 species, followed by the 0 - 10 cm depth with 110 species. Layers from 10 - 20 cm depth and 20 - 30 cm depth had 77 and 45 species, respectively, showing a decreasing ant richness structure in the soil profile with depth.

The preliminary checklist of soil ants from the Colombian Amazon region (Table 2), contains 218 species distributed in 60 genera of 10 subfamilies. The richest subfamily was Myrmicinae with 99 species, followed by Ponerinae with 41 species. Other subfamilies found there included Formicinae with 31 species, Ectatomminae with 18 species, Dolichoderinae with 14 species, Pseudomyrmecinae with 6 species, Dorylinae with 5 species, Amblyopone with 2 species and the Paraponerinae and Proceratiinae with 1 species each, respectively. The richest genus was Pheidole Westwood, 1839 with 27 species, followed by Crematogaster Lund, 1831 with 16 species. Other genera rich in species are Camponotus Mayr, 1861 with 14 species, Odontomachus Latreille, 1804 with 10 species and Gnamptogenys Roger, 1863 with 8 species.

Checklist of the soil ant species of the Colombian Amazon. The list is organised alphabetically by subfamily, genus and species. Species names in bold characters refer to species recorded for the first time in Colombia. River basins corresponded to: A = Amazon river; C = Caquetá river; P = Putumayo river. Depth of species collection: 1 = Litter; 2 = 0 – 10 cm; 3 = 10 – 20 cm; 4 = 20 – 30 cm. Land use corresponded to PF = Primary forest; SF = Secondary forest; P = Pasture; R = Young secondary regeneration forest.

Subfamilies Scientific valid name River basin Depth Land use
Amblyoponinae Prionopelta antillana Forel, 1909 A,C 1,2,3 R
Fulakora orizabana (Brown, 1960) C 3 P
Dolichoderinae Azteca sp1 C,P 1,2 PF
Azteca sp2 A,P 1,2,4 PF, R
Azteca sp3 A,C,P 1,2,3,4 PF, P, R
Azteca sp4 C 2,3 SF
Azteca sp5 C 1,4 PF, P
Dolichoderus attelaboides Fabricius, 1775 A 1 PF
Dolichoderus bidens Linnaeus, 1758 C,P 1,2 P, R
Dolichoderus bispinosus Olivier, 1792 P 1 PF, R
Dolichoderus imitator Emery, 1894 A,C 2,3 R
Dolichoderus quadridenticulatus Roger, 1862 C 2 P
Dolichoderus rugosus Smith, 1858 A,P 1 PF, R
Linepithema sp1 A,C,P 1,2,3,4 PF, P
Linepithema sp2 C 1,2,3,4 PF, P, SF
Linepithema sp3 C 1,2,3,4 PF, SF
Dorylinae Cheliomyrmex andicola Emery, 1894 C 2 SF
Eciton hamatum Fabricius, 1782 A,C 1 PF
Labidus praedator Smith, 1858 C 1,2,3 P, SF
Leptanilloides sp. P 2 PF
Neivamyrmex cf. hetschkoi Mayr, 1886 C 1,4 PF, SF
Ectatomminae Ectatomma brunneum Smith, 1858 A,C 1 PF, R
Ectatomma edentatum Roger, 1863 A 2 PF
Ectatomma lugens Emery, 1894 P 2 PF
Ectatomma ruidum Roger, 1860 A,C 1,2,3 PF, P
Ectatomma tuberculatum Olivier, 1792 C 2 PF
Gnamptogenys cf. ilimani Lattke, 1995 C 1 P
Gnamptogenys (gr. minuta) sp A 2 PF
Gnamptogenys kempfi Lenko, 1964 A 2 PF
Gnamptogenys cf. lavra Lattke, 2002 A,C,P 1,2 PF
Gnamptogenys porcata Emery, 1896 C 3 R
Gnamptogenys striatula Mayr, 1884 C,P 1,3 PF
Gnamptogenys strigata Norton, 1868 P 3 PF
Gnamptogenys tortuolosa Smith, 1858 A 1 PF
Typhlomyrmex clavicornis Emery, 1906 C,P 3,4 PF, SF
Typhlomyrmex major Santschi, 1923 A,C 3,4 PF, SF
Typhlomyrmex meire Lacau, Villemant & Delabie, 2004 C 1,3 P
Typhlomyrmex pusillus Emery, 1894 C 1,2,4 PF, P
Typhlomyrmex sp. A,C 2 PF
Formicinae Acropyga aff. epedana Snelling, 1973 C 2 R
Acropyga exsanguis Wheeler, 1909 C 4 PF, R
Acropyga goeldii Forel, 1893 C,P 1,2,3 PF
Acropyga guianensis Weber, 1944 P 1,2,3 PF, P, SF
Acropyga smithii Forel, 1893 P 2 PF
Acropyga tricuspis LaPolla, 2004 A 1,2 R
Brachymyrmex aff. heeri Forel, 1874 P 2 PF
Brachymyrmex aff. australis Forel, 1901 C 1 P
Brachymyrmex cordemoyi Forel, 1895 A,C 1,2,3,4 PF, SF
Brachymyrmex myops Emery, 1906 A 2 PF
Brachymyrmex pictus Mayr, 1887 C 1 SF
Camponotus aff. ager Smith, 1858 A 2 PF
Camponotus atriceps Smith, 1858 A 1 PF
Camponotus casicus Santschi, 1920 C 1 SF
Camponotus femoratus Fabricius, 1804 A,C,P 1,2 PF, P, SF
Camponotus latangulus Roger, 1863 C 1 P
Camponotus nitidior Santschi, 1921 C 2 PF
Camponotus novogranadensis Mayr, 1870 A 1 PF
Camponotus rapax Fabricius, 1804 C 1,3 PF
Camponotus rufipes Fabricius, 1775 C 1 PF
Camponotus senex Smith, 1858 C 3 P
Camponotus sp1 C 1 SF
Camponotus sp2 A 1 PF
Camponotus sp3 C 3 R
Camponotus sp4 C 2 PF
Gigantiops destructor Fabricius, 1804 A,C,P 1,2 PF
Myrmelachista sp. C 1,3 PF
Nylanderia sp1 A 1,2,3,4 PF, P, R, SF
Nylanderia sp2 A,C 1,2,3,4 PF, P, S, SF
Nylanderia sp3 A 1,2 PF, P, SF
Nylanderia sp4 A 3 PF
Myrmicinae Acromyrmex coronatus Fabricius, 1804 C 4 PF
Apterostigma auriculatum Wheeler, 1925 P 2 PF
Apterostigma cf. acre Lattke, 1997 A 1 R
Apterostigma goniodes Lattke, 1997 C 1,4 PF
Apterostigma (gr. pilosum) sp.1 A 1 SF
Apterostigma (gr. pilosum) sp.2 C 2 R
Apterostigma megacephala Lattke, 1999 C 1 P
Atta colombica Guérin-Méneville, 1844 C 1 PF, P
Blepharidatta brasiliensis Wheeler, 1915 A 1 PF
Cardiocondyla nuda Mayr, 1866 C 1 SF
Carebara brevipilosa Fernández, 2004 C 3 P
Carebara (gr. escherichi) sp.1 A 4 PF
Cephalotes aff. cordatus Smith, 1853 C 2 P
Cephalotes atratus Linnaeus, 1758 A,C 1 PF
Cephalotes cf. patellaris Mayr, 1866 C 1 SF
Cephalotes spinosus Mayr, 1862 C 1,3 P
Crematogaster abstinens Forel, 1899 A,C 1,2,3,4 SF
Crematogaster acuta Fabricius, 1804 A 2,3,4 PF
Crematogaster aff. evallans Forel, 1907 C 2,3 SF
Crematogaster brasiliensis Mayr, 1878 A,C 1,2,3,4 PF, S, SF
Crematogaster bryophilia Longino, 2003 A 1 PF
Crematogaster carinata Mayr, 1862 A,C,P 1,2,3,4 PF, P, SF
Crematogaster cf. snellingi Longino, 2003 A 1 PF
Crematogaster crinosa Mayr, 1862 C 1,3,4 SF
Crematogaster erecta Mayr, 1866 C 2 SF
Crematogaster flavosensitiva Longino, 2003 P 1 PF
Crematogaster limata Smith, 1858 A,C,P 1,2,3,4 PF, P, R, SF
Crematogaster longispina Emery, 1890 A,C 1,2 PF, SF
Crematogaster minutissima Mayr, 1870 A 1,2 PF
Crematogaster nigropilosa Mayr, 1870 A 2,3 PF
Crematogaster sotobosque Longino, 2003 C,P 2,3 PF
Crematogaster tenuicula Forel, 1904 A,P 1,2,3 PF, R
Cyphomyrmex bicornis Forel, 1895 A 2 PF
Cyphomyrmex laevigatus Weber, 1938 A,P 1 PF, R
Cyphomyrmex minutus Mayr, 1862 C 1 PF
Cyphomyrmex peltatus Kempf, 1966 C 1 PF
Cyphomyrmex rimosus Spinola, 1851 C,P 1,2,3 PF, P, SF
Hylomyrma immanis Kempf, 1973 A,C 1,2 PF, SF
Hylomyrma sagax Kempf, 1973 C 1 PF
Kempfidris inusualis Fernández, 2007 A 2 R
Megalomyrmex cf. balzani Emery, 1894 C 3 PF
Megalomyrmex emeryi Forel, 1904 C,P 1,2,3 P
Megalomyrmex foreli Emery, 1890 C,P 1,2,3 PF, P
Megalomyrmex leoninus Forel, 1885 C 1,3 P
Megalomyrmex megadrifti Boudinot, Sumnicht & Adams, 2013 C 1 PF
Mycocepurus smithii Forel, 1893 A,C,P 1,2,3 PF, SF
Myrmicocrypta longinoda Weber, 1938 A 2 PF
Myrmicocrypta sp. C 2 PF
Myrmicocrypta spinosa Weber, 1937 C 3 PF
Nesomyrmex tristani Emery, 1896 C 1 SF
Ochetomyrmex neopolitus Fernández, 2003 P 1 PF
Octostruma balzani Emery, 1894 C 1 PF
Octostruma impressa Palacio, 1997 C 1 PF
Pheidole aff. biconstricta Mayr, 1870 A,C,P 1,2 PF
Pheidole aff. chocoensis Wilson, 2003 P 1,3 PF
Pheidole aff. cocciphaga Borgmeier, 1934 A 1 PF
Pheidole aff. huilana Wilson, 2003 P 1,2 PF, R
Pheidole aff. radoszkowski Mayr, 1884 C 3 SF
Pheidole aff. sensitiva Borgmeier, 1959 P 1 PF
Pheidole aff. subnuda Wilson, 2003 P 1 PF
Pheidole aff. vafra Santschi, 1923 C 2 SF
Pheidole astur Wilson, 2003 P 3 PF
Pheidole gertrudae Forel, 1886 C 3 PF
Pheidole sp1 C 1 SF
Pheidole sp2 A,C 1,2,3 PF, SF
Pheidole sp3 P 2 PF
Pheidole sp4 P 4 PF
Pheidole sp5 C 3 P, SF
Pheidole sp6 C 1 SF
Pheidole sp7 C 2 SF
Pheidole sp8 P 2,3 PF
Pheidole sp9 C 3,4 P, SF
Pheidole sp10 C 2 P
Pheidole sp11 C 2 SF
Pheidole sp12 C 1 SF
Pheidole sp13 C 2 P
Pheidole sp14 A,P 2,3,4 PF, SF
Pheidole sp15 A,P 1,3 PF
Pheidole sp16 A 1,2,4 PF
Pheidole sp17 A 4 R
Procryptocerus scabriusculus Forel, 1899 C 3 PF
Rogeria belti Mann, 1922 A 1,2 PF, P
Sericomyrmex bondari Borgmeier, 1937 A,C 1,2,3,4 PF, R
Solenopsis geminata Fabricius, 1804 C 1,2,3 PF, P, R
Solenopsis sp1 C 1 P
Solenopsis sp2 P 2 PF
Solenopsis sp3 A,P 1,2,4 PF, P, SF
Solenopsis sp4 A,C 1,3 PF, P, SF
Solenopsis sp5 C 1,2,3,4 PF, P, SF
Solenopsis virulens Smith, 1858 P 1 PF
Strumigenys denticulata Mayr, 1887 A 1 R
Strumigenys interfectiva Lattke & Goitía, 1997 C 1 PF
Strumigenys smithii Forel, 1886 P 1 PF
Trachymyrmex sp1 C,P 1,2,3,4 PF, SF
Trachymyrmex sp2 A,C,P 1,2,3,4 PF, P, SF
Trachymyrmex sp3 C,P 1,3,4 PF, SF
Trachymyrmex sp4 C 3 PF
Tranopelta gilva Mayr, 1866 A,C,P 1,2,3,4 PF, P, SF
Wasmannia auropunctata Roger, 1863 A,C,P 1,2,3,4 PF, P, R, SF
Paraponerinae Paraponera clavata Fabricius, 1775 C,P 1 PF
Ponerinae Anochetus diegensis Forel, 1912 C 1 PF, P
Anochetus mayri Emery, 1884 C 1 PF
Anochetus cf. neglectus Emery, 1894 C 1 PF
Centromyrmex alfaroi Emery, 1890 C 2 SF
Centromyrmex brachycola Roger, 1861 A,C,P 1,2,3 PF, P, SF
Cryptopone guianensis Weber, 1939 C,P 2,3 PF
Cryptopone holmgreni Wheeler, 1925 C 2,3,4 PF, SF
Dinponera longipes Emery, 1901 A 1 PF
Hypoponera distinguenda Emery, 1890 A,C 1,2 PF, R
Hypoponera sp1 C,P 1,2,3,4 PF, P, R
Hypoponera sp2 C,P 1,2,3 PF, R
Hypoponera sp3 A,P 1,2 PF, R
Hypoponera sp4 A,C,P 1,2,3 PF, SF
Hypoponera sp5 A,C,P 1,2,3 PF, P, SF
Hypoponera sp6 A,C 1,2,3,4 PF, P, R, SF
Leptogenys (gr. crudelis) sp C 1 PF
Mayaponera constricta Mayr, 1884 A,C,P 1,2 PF, P, R, SF
Neoponera apicalis Latreille, 1802 A,C 1 PF
Neoponera commutata Roger, 1860 P 2 PF
Neoponera unidentata Mayr, 1862 C 3 PF
Neoponera verenae Forel, 1922 C 1 PF
Neoponera villosa Fabricius, 1804 A 1 PF
Odontomachus aff. panamensis Forel, 1899 C 1 SF
Odontomachus bauri Emery, 1892 C 1,4 PF, P
Odontomachus bradleyi Brown, 1976 C 1 PF
Odontomachus caelatus Brown, 1976 P 1 PF
Odontomachus haematodus Linnaeus, 1758 A,C 1 SF
Odontomachus meinerti Forel, 1905 C 1,3 PF
Odontomachus opaciventris Forel, 1899 C,P 1 PF, SF
Odontomachus scalptus Brown, 1978 C 1 SF
Odontomachus spisuss Kempf, 1962 P 1 PF
Odontomachus cf. yucatecus Brown, 1976 C 2 PF
Pachycondyla crassinoda Latreille, 1802 P 1,2 PF
Pachycondyla fuscoatra Roger, 1861 A 1 R
Pachycondyla harpax Fabricius, 1804 A,C,P 1,2 PF, R
Pachycondyla impressa Roger, 1861 A 1 PF
Pseudoponera stigma Fabricius, 1804 A,C,P 1,2 PF
Rasopone arhuaca Forel, 1901 A,C,P 1,2,3 PF, R
Rasopone becculata MacKay & MacKay, 2010 C 2,3,4 PF
Rasopone lunaris Emery, 1896 A 2 PF
Rasopone sp. C 2 PF
Proceratiinae Proceratium transitionis de Andrade, 2003 C 1 PF
Pseudomyrmecinae Pseudomyrmex sp1 A,C 1,2,3 PF, P
Pseudomyrmex sp2 A,C,P 1,2,4 PF, P, R, SF
Pseudomyrmex sp3 C 1,2,3,4 PF, P, SF
Pseudomyrmex sp4 C,P 1,2,4 PF, SF
Pseudomyrmex sp5 C 1,3,4 P, SF
Pseudomyrmex sp6 C,P 1 PF

Ant richness in this report is remarkable when compared with previous reports from the Amazon region. The study done by Ryder Wilkie et al. 2010, which is recognised as the most complete work on ant diversity in the Amazon region, recorded at the Tiputini Reserve in Ecuador 66 genera and more than 300 species between subsoil and canopy. The high diversity reported in our work was certainly the effect of the broad area sampled (which includes three river basins) and the wide range of altitude included (Marsh et al. 2018).

The following are new records for Colombia:

Acropyga tricuspis (LaPolla, 2004)

Specimen Data. 4 w. AMAZONAS. Puerto Nariño [03°46'33.6"S; 70°21'41.8"W], 84 m a.s.l., 16 Jun 2017, C. Peña. Identification by D. Castro & A. Meneses (CATAC - 0413).

Comments. New record for Colombia. This species has been recorded in the Brazilian Amazonia (LaPolla 2004).

Typhlomyrmex clavicornis (Emery, 1906)

Specimen Data. 3 w. CAQUETÁ. Belén de los Andaquies [01°42'06.8"N; 75°53'57.5"W], 1500 m a.s.l., 23 Jan 2016, D. Castro. Identification by D. Castro & S. Sanchez (CATAC - 0893); 8 w, CAQUETÁ. Florencia, Palmichar [01°42'52.2"N; 75°36'53.6"W], 241 m a.s.l., 23 Mar 2016, Y. Virguez. Identification by D. Castro & S. Sanchez (CATAC - 0292).

Comments. New record for Colombia. This species has been recorded in Bolivia (Type locality), Brazil, French Guiana, Guyana, Paraguay and Suriname (Fernández and Arias-Penna 2008, Wild 2007).

Typhlomyrmex meire (Lacau, Villemant & Delabie, 2004)

Specimen Data. 2 w. CAQUETÁ: Florencia, Sebastopol [01°43'00.12"N; 75°36'49.3"W], 527 m a.s.l., 29 Mar 2016, Y. Virguez. Identification by D. Castro & S. Sanchez (CATAC-02563).

Comments. New record for Colombia. This species has been recorded in Brazil (Lacau et al. 2004).

Cyphomyrmex bicornis (Forel, 1895)

Specimen Data. 1 w. AMAZONAS. Leticia. Tanimboca Natural Reserve, [04°07'15.4"S - 69°57'19.7"W], 98 m a.s.l., 23 Jun 2017, D. Castro. Identification by M. Tocora (CATAC-01582).

Comments. New record for Colombia. This species has been recorded in Brazil (Type Locality) (Kempf 1966).

Megalomyrmex emeryi (Forel, 1904)

Specimen Data. 6 w. CAQUETÁ. Florencia, Sebastopol [01°43'00.12"N; 75°36'49.3"W], 527 m a.s.l., 29 Mar 2016, Y. Virguez. Identification by M. Tocora (CATAC-0326).

Comments. New record for Colombia. This species has been recorded in Bolivia, French Guiana, Guyana, Peru and Suriname (Type Locality) (Brandão 2003, Brandão 1990).

Myrmicocrypta spinosa (Weber, 1937)

Specimen Data. 1 w. CAQUETÁ. Florencia, Arandia [01°26'39.9"N - 75°31'29.1"Wˈ], 259 m a.s.l., 2 Jul 2016, Y. Virguez. Identification by M. Tocora (CATAC-0331).

Comments. New record for Colombia. This species has been recorded in Guyana (Type Locality) (Weber 1937).

Discussion

The Caquetá river basin showed the highest number of soil ant species amongst basins (149 species, which corresponded to 68% of the total ants recorded), followed by the Amazon river basin (86 species, 40%) and the Putumayo river basin (71 species, 33%). From all species recorded, 89 species were exclusively registered in the Caquetá river basin, which was twice the number of species reported exclusively in the Amazon river basin (36 species) and in the Putumayo river basin (25 species). The high diversity of the Caquetá river basin may be areflection of the geographic conditions of the area and the sampling effort used there. The Caquetá river basin includes the Andean-Amazonian transition where a high turnover of species might occur, but additionally, it was the one with the greatest sampling effort.

From all the ant species recorded, 20 species were found in the four soil depths (Table 2). The most abundant of these species were: Wasmannia auropunctata Roger, 1863, Tranopelta gilva Mayr, 1866, Sericomyrmex bondari Borgmeier, 1937, Crematogaster limata Smith, 1858, Crematogaster carinata Mayr, 1862, Crematogaster brasiliensis Mayr, 1878, Crematogaster abstinens Forel, 1899 and Brachymyrmex cordemoyi Forel, 1895.

The genera Acropyga and Typhlomyrmex are underground genera commonly collected at deep soil depths. For example, the genus Typhlomyrmex was collected mostly at 10 - 20 and 20 – 30 cm soil depths. Although TSBF was appropriate for collecting these soil ants, which are generally undersampled with other methods of collection, the TSBF method might underestimate army ants and other large ants such as Paraponerinae that were not recorded in the searched Amazon basin area (Ryder Wilkie et al. 2007, Oliveira and Morato 2009, Sanabria-Blandón and de Ulloa 2011, Ryder Wilkie et al. 2010). However, the use of soil monoliths for macrofauna sampling allowed comparisons between macrofauna groups (e.g. ants with other macrofauna groups). The use of more than one method to obtain an accurate image of the community of ants has been proven (e.g. Winkler or pitfall for estimating the abundance of leaf litter ants) (Delsinne and Arias-Penna 2012, Wong and Guénard 2017, Ryder Wilkie et al. 2007). However, in this study, the composition of subterranean ant subfamilies was not affected by the method of collection used (TSBF) as the proportions of subfamilies were similar to those obtained using additional methods (Wong and Guénard 2017).

An important number of canopy and arboreal ant species such as Crematogaster, Azteca, Dolichoderus, Camponotus and Cephalotes were collected. Studies of ant fauna in the Colombian Amazon (Pérez et al. 2009) highlighted the diversity of these genera in the region. Canopy and arboreal ant species can be an important part of the ant density in the upper strata of soil (leaf litter and the depth of 0 - 10 cm) as occurred in this study where most of these ants were collected in litter. Results inferred that these ants use the soil as a way for transportation or for food provisioning, although they do not live in the soil such as ants of the genera Pheidole, Acropyga, Cryptopone or Hypoponera.

Some arboreal species of Crematogaster, Camponotus, Myrmelachista, Procryptocerus and Pseudomyrmex were found in soil deep horizons, even at 20-30 cm depth such as Azteca and Pseudomyrmex. Ant collection was done manually in the field. During this process, some arboreal ants could fall down and contaminate monolith samples when the bags were not well closed. However, arboreal ants may realistically be away from their common substrate or nest, as little is known about their biology, even more so when this is not the first time they have been recorded in soil samples (Rosumek et al. 2008, Vasconcelos et al. 2003, Delabie and Fowler 1995).

Ants are the most diverse soil macrofauna group in the Amazon region (Mathieu et al. 2005Barros et al. 2008) and represent a high density (Table 3). In the Caquetá basin, they are the most dense organisms of the soil macrofauna. In the other two river basins, ants are only exceeded by termites. Differences in ant and termite densities might be a reflection of the land use sampled. Termites tend to be more abundant in less disturbed ecosystems (Mboukou-Kimbatsa et al. 1998, Velásquez et al. 2012), while ants tend to be more abundant in disturbed or degraded ecosystems of the Amazon region. In our study, the Caquetá basin is where the most disturbed coverings, such as pastures and young secondary forests, are found (Table 1) (Aquino et al. 2008, Barros et al. 2002, Marichal et al. 2014, Pinzón et al. 2014, Rousseau et al. 2014).

Density (Individuals/m2) of the main taxonomic groups collected in TSBF monoliths in the Colombian Amazon.

Taxa Group River Basin
Amazonas Caquetá Putumayo
Formicidae 274.16 173.70 82.12

Amblyoponinae

0.78 0.49 0.00

Dolichoderinae

4.39 9.66 3.29

Dorylinae

0.00 2.47 0.47

Ectatomminae

7.24 4.23 3.29

Formicinae

72.87 37.94 9.18

Myrmicinae

145.74 91.96 45.88

Paraponerinae

0.00 0.63 0.71

Ponerinae

41.34 24.26 18.82

Proceratiinae

0.00 0.07 0.00

Pseudomyrmecinae

1.29 1.97 0.47
Termitoidea 289.15 146.33 142.12
Coleoptera 33.59 15.80 34.12
Araneae 26.36 15.87 17.18

Immature insects

18.35 15.02 13.18
Chilopoda 15.50 8.32 12.00
Diplopoda 11.11 6.98 9.41
Blattodea 4.13 7.62 4.94
Hemiptera 9.30 5.71 5.18
Isopoda 8.79 3.74 8.94
Diplura 8.01 3.95 4.00
Opiliones 8.01 3.10 4.24

The Neotropics (including the Amazon basin) have been recognised as a region of speciation and conservation of multiple lineages of ants (Moreau and Bell 2013). Results presented here increase the knowledge of soil ants from the Amazon region and suggest that ant species richness may increase considerably when sampling effort increases and combined methodologies are used to capture ants in different habitats.

Acknowledgements

We thank the Instituto Amazónico de Investigaciones Científicas SICHI for its financial support. Thanks to AZICATCH, AIZA and OIMA indigenous associations and the Tanimboca, Otra Parte and Cerca Viva Natural Reserves for allowing us to collect ants in their areas. We also thank Jack Longino, Claudia Ortiz and Lina Pedraza for their help in the identification of some ant species. We heartily thank Dr. John Lattke and Dr. Gabriela Camacho for their useful comments and suggestions on the manuscript.

Author contributions

DC and CPPV collected the material. DC and FF analysed data. DC, FF, ADM, MCT and SS identified the material. DC, FF, MCT and CPPV elaborated the manuscript.

References