Biodiversity Data Journal : Data Paper (Biosciences)
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Data Paper (Biosciences)
Photo images, 3D/CT data and mtDNA of the freshwater mussels (Bivalvia: Unionidae) in the Kyushu and Ryukyu Islands, Japan, with SEM/EDS analysis of the shell
expand article infoYuichi Kano, Yoshihisa Kurita , Kazuki Kanno, Kengo Saito, Hironori Hayashi, Norio Onikura, Takeshi Yamasaki§
‡ Kyushu University, Fukuoka, Japan
§ Yamashina Institute for Ornithology, Konoyama, Japan
Open Access

Abstract

Background

Freshwater mussels (Bivalvia: Unionidae), which are keystone species of freshwater ecosystems, are in global decline. In addition to ecological/genetic studies, morphological examinations are needed to help provide information for the development of additional freshwater mussel studies and eventually conservation efforts for freshwater ecosystems.

The microscopic structure, which can be obtained by scanning electron microscopy (SEM) and elemental composition, which can be obtained with energy dispersive X-ray spectrometry (EDS), of mollusc shells are of interest to malacologists. However, information about freshwater mussels is still limited.

Kyushu Island is the southernmost island of the four major islands of Japan. Kyushu Island is a hotspot of bitterling fishes in Japan, which simultaneously means that the island is a hotspot of freshwater mussels. The Ryukyu Islands stretch southwest from Kyushu Island to Taiwan; a freshwater mussel of unknown origin was reported from the Ryukyu Islands.

Digital archiving for biology and ecology is a continuing challenge for open science. This data paper describes online published photo images, 3D/CT and mtDNA data and SEM/EDS analyses of the shell of freshwater mussels that inhabit the Kyushu and Ryukyu Islands, Japan. Our data will provide basic information regarding freshwater biology and be of public interest as open science.

New information

Photo images, 3D/CT data, mtDNA data, SEM images and EDS elemental analysis of freshwater mussels that inhabit the Kyushu and Ryukyu Islands (61 individuals, nine species/subspecies) were published online in a local database (http://ffish.asia/Unionidae3D), GBIF (http://ipt.pensoft.net/resource?r=unionidae3d) and DDBJ/EMBL/Genbank (LC431810–LC431840).

Keywords

3D model, anatomy, CT scan, digital archiving, elemental composition, energy dispersive X-ray spectrometry (EDS), freshwater mussels morphology, open science, scanning electron microscope (SEM), shell exoskeleton

Introduction

Freshwater mussels (Bivalvia: Unionidae) are one of the most endangered freshwater animals in Japan and around the world. In total, 21 unionid species/subspecies have been reported from Japan, including introduced Hyriopsis cumingii from China (Masuda and Uchiyama 2004, Imai 2008, Kondo 2008, Kitano and Imai 2012), of which 13 species/subspecies are listed as endangered (NT, VU or CR+EN) by the Red Data Book of the Ministry of the Environment, Japan (2018). Freshwater mussels have an ecological relationship with bitterling fishes, which are also generally threatened. Bitterling fishes lay their eggs in the gills of mussels; the life history/cycle of bitterling fishes is not completed without mussels (e.g. Kitamura et al. 2012). On the other hand, mussel larvae (glochidia) parasitise sympatric fishes, including bitterling fishes (Kondo 2008). Freshwater mussels also provide crucial ecosystem services by contributing to water clarity (e.g. Chowdhury et al. 2016). Thus, freshwater mussels are considered keystone species and more studies are needed to help provide information for conservation plans for freshwater ecosystems.

Kyushu Island is a hotspot for bitterling fishes in Japan (Onikura et al. 2011), which also means that the island is home to populations of freshwater mussels. The Ryukyu Islands stretch southwest from Kyushu Island to Taiwan; a freshwater mussel, Cristaria tenuis, was once reported from Ishigaki Island, one of the major Ryukyu Islands (Imai 2008). Cristaria tenuis was also found in Fukuoka Prefecture, which is in the northern part of Kyushu Island (Kitano and Imai 2012). Further studies are needed to determine whether C. tenuis is native to Ishigaki Island or Fukuoka Prefecture (Imai 2008).

Shell exoskeleton morphology is a crucial characteristic in mollusc studies related to hydrodynamics (e.g. Eagar 1978, Aldridge 1999, Denny and Blanchette 2000, Kondo 2008), whereas evaluation of the soft body has usually been overlooked. In addition to shell morphology, microscopic structure and chemical/elemental composition of the shell, which can be obtained by scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS), have been of interest to malacologists (Matsuda and Hirano 1980, Carter 1990, Ubukata 1994, Fuchigami and Sasaki 2005, de Paula and Silveira 2006, Checa et al. 2016).

Digital archiving is one of the progressive challenges for the open science of biology and biodiversity studies (e.g. Berquist et al. 2012, Miyazaki et al. 2014, Kano et al. 2016, Kano et al. 2018). This paper describes photo images published online, 3D/CT data (to evaluate both the shell exoskeleton and soft body) and mtDNA data of freshwater mussels that inhabit the Kyushu and Ryukyu Islands of Japan (61 individuals of nine species/subspecies) with SEM images and EDS analysis of the shell. We expect our study will contribute valuable information to general malacology, freshwater ecosystem conservation and public interest in biodiversity conservation.

Sampling methods

Study extent: 

Freshwater mussels were collected in the wild of Kyushu and Ryukyu Islands, Japan (Fig. 1).

Figure 1.  

Sampling sites of the nine freshwater mussel species in the Kyushu and Ryukyu Islands. The number attached to each circle indicates the number of individuals.

Sampling description: 

The living specimens (61 individuals) were captured by hand from the Tsuri, Tenkai, Yamakuni, Matsuura, Katsura, Chikugo, Kikuchi and Nagura River systems.

Quality control: 

Identification followed Masuda and Uchiyama (2004), Kondo (2008) and Sano et al. (2017).

Step description: 

Individual photo images were taken in the field (Fig. 2) (Kano and Nakajima 2014). The specimens were fixed in 10% formalin followed by preservation in 70% ethanol. A small segment of the soft body was cut off and separately preserved in 99% ethanol for mtDNA analysis.

Figure 2.  

A live Nodularia douglasiae nipponensis (QUYK-08668d)

All specimens were CT scanned (Aloka Latheta LCT-200, Hitachi Ltd., Japan) and 3D surface models (Fig. 3; CT value: −450 to 600) were extracted from the CT data (Figs 4, 5).

Figure 3.

3D surface models of freshwater mussels.

aInversiunio yanagawensis (QUYK-06858) with "metal artifacts" noise due to the thick/hard shell.  
bLanceolaria grayana (QUYK-08773d).  
cPronodularia japanensis (QUYK-08881).  
dNodularia douglasiae nipponensis (QUYK-08667d).  
Figure 4.

CT digital image of a freshwater mussel (Sinanodonta japonica; QUYK-08891).

aExternal shell exoskeleton.  
bDigitally sliced shell that shows the internal soft body.  
Figure 5.  

A CT-scanned image of Obovalis omiensis (QUYK-08887).

mtDNA analysis of 16S-rRNA was conducted for 31 individuals (Fig. 6). For PCR amplification, we used the primer pair Unio16SFwd (forward: 5′-TGCCTGTTTACCAAAAACATCG-3′) and Unio16SRev (reverse: 5′-CTTGGGGTCCTTTCGTACA-3′). PCR amplification was performed in 10-µl reaction mixtures that contained 5 µl KAPA 2G™ Robust HotStart ReadyMix (Kapa Biosystems, USA), 1 µM of each primer, 1 µl DNA template and 2 µl sterile deionised water. The reaction mixtures were preheated at 95°C for 3 min, followed by 30 amplification cycles (95°C for 15 s, 50°C for 15 s and 72°C for 40 s), with a final 5-min extension at 72°C. Direct sequencing of the PCR products was conducted externally (FASMAC, Japan). The nucleotide sequences were deposited in DDBJ/EMBL/GenBank (accession numbers: LC431810–LC431840).

Figure 6.  

A phylogenetic tree of nine Unionidae species/subspecies found in the Kyushu and Ryukyu Islands (mtDNA 16S-rRNA). The tree was reconstructed using the maximum likelihood method (model: GTR+G) in MEGA 7 (Kumar et al. 2016). Bootstrap values were obtained with 500 pseudoreplicates. Margaritifera falcata was used as an outgroup. The DDBJ accession numbers are shown in brackets.

The SEM/EDS analysis was conducted for 29 individuals. A shell fragment was cut off from each specimen (from the posterior part of the shell) and the inner side of the shell was analysed by SEM (JCM-6000, JEOL Ltd., Japan) to observe the microscopic images of the pearled surface (Fig. 7). Furthermore, EDS analysis (JED-2300, JEOL Ltd.) was conducted to determine the elemental composition of the shell fragment by targeting B, C, N, O, F, Na, Mg, Al, Si, P (Fig. 8a), S, Cl, K and Ca (Fig. 8b) (Suppl. material 1).

Figure 7.

SEM images of the shell (inner pearled surface).

aMultilevel wavelike structure of Sinanodonta japonica QUYK-08891 (Scale bar 100 μm).  
bMultilevel wavelike structure of Obovalis omiensis QUYK-08872 (Scale bar 20 μm).  
cHexagonal microstructure of Inversidens brandtii QUYK-08915 (Scale bar 5 μm).  
dColumnar microstructure of Pronodularia japanensis QUYK-08880 (Scale bar 5 μm).  
Figure 8.

Elemental compositions of the shell (% number of atoms) discriminated by locality.

aPhosphorous.  
bCalcium.  

Geographic coverage

Description: 

Freshwater habitats of Kyushu and Ryukyu Islands, Japan.

Coordinates: 

24.4 and 33.8 Latitude; 124.2 and 131.2 Longitude.

Taxonomic coverage

Description: 

All freshwater mussels (family Unionidae), distributed in the Kyushu and Ryukyu Islands, were studied except Anemina arcaeformis, Hyriopsis schlegelii and Sinanodonta sp. A shell exoskeleton, that was photographically identified as A. arcaeformis, was reported from Miyazaki Prefecture, Kyushu Island (Toyama and Nishi 2016), although all other details remain unclear. Hyriopsis schlegelii individuals were artificially introduced to Isahaya Bay for water purification (Ishizaki et al. 2007), even though H. schlegelii is non-native to Kyushu Island. Sinanodonta sp. (or spp.) populations were informally reported from the Ryukyu Islands, but some of the populations were likely introduced from Taiwan (Shokita 1984, Kurozumi 2003, Imai 2008).

Taxa included:
Rank Scientific Name Common Name
kingdom Animalia Animals
phylum Mollusca Molluscs
class Bivalvia Bivalves
order Unionoida Freshwater mussels and pearl mussels
family Unionidae Freshwater mussels
species Cristaria tenuis "Dobu-gai-modoki"
species Inversidens brandtii "Oba-eboshi-gai"
species Inversiunio yanagawensis "Nise-matsukasa-gai"
species Lanceolaria grayana "Tongari-sasanoha-gai"
subspecies Nodularia douglasiae nipponensis "Ishi-gai"
species Obovalis omiensis "Kataha-gai"
species Pronodularia japanensis "Matsukasa-gai"
species Sinanodonta japonica "Ta-gai"
species Sinanodonta lauta "Numa-gai"

Temporal coverage

Notes: 

From 2013-12-21 to 2018-06-29.

Usage rights

Use license: 
Other
IP rights notes: 

Data resources

Data package title: 
Photo images, 3D/CT data and mtDNA of the freshwater mussels (Bivalvia: Unionidae) in the Kyushu and Ryukyu Islands, Japan, with SEM/EDS analysis of the shell.
Number of data sets: 
2
Data set name: 
Unionidae3D
Character set: 
UTF8
Data format: 
html; jpg; Wavefront object format (.obj); CT dicom file (.dcm); text for mtDNA sequence.
Description: 

Photo images, surface 3D models and CT scanned data are available for 61 individuals. To render the CT dicom files as a visual 3D volume, several free software are available. mtDNA sequences (16S-rRNA) are also available for 31 individuals. SEM images and EDS analysis are available for 29 individuals. Below, the main 11 columns are listed;

Column label Column description
Specimen/Data ID ID for the specimen
Images Images for specimen and associated files
Species Species information
Taxon Order, family and genus
DNA DNA sequence data if available
N Number of individuals
Country Country where the sample was obtained
Sampling location Description of the locality where the specimen was obtained with a map (the resolution of locality data is shown roughly to prevent poaching)
Location Locality information
Habitat Habitat information
Comment Other information
Data set name: 
Photo images, 3D/CT data and mtDNA of the freshwater mussels (Bivalvia: Unionidae) in the Kyushu and Ryukyu Islands, Japan, with SEM/EDS analysis of the shell
Data format: 
Darwin Core Archive
Description: 

GBIF registered occurrence and multimedia data for the specimens. Below, the 42 columns are listed;

Column label Column description
occurrenceID (occurrence) Occurrence ID
basisOfRecord (occurrence) The specific nature of the data record
eventDate (occurrence) The date-time or interval during which the specimen collected
year (occurrence) The four-digit year in which the Event occurred, according to the Common Era Calendar
month (occurrence) The ordinal month in which the Event occurred
day (occurrence) The integer day of the month on which the Event occurred
eventRemarks (occurrence) Comments or notes about the Event
scientificName (occurrence) Scientific name (or tentative name) of the specimen
kingdom (occurrence) The full scientific name of the kingdom in which the taxon is classified
phylum (occurrence) The full scientific name of the phylum or division in which the taxon is classified
class (occurrence) The full scientific name of the class in which the taxon is classified
order (occurrence) The full scientific name of the order in which the taxon is classified
family (occurrence) The full scientific name of the family in which the taxon is classified
genus (occurrence) The full scientific name of the genus in which the taxon is classified
specificEpithet (occurrence) The name of the first or species epithet of the scientificName
infraspecificEpithet (occurrence) The name of the lowest or terminal infraspecific epithet of the scientificName, excluding any rank designation
taxonRank (occurrence) The taxonomic rank of the most specific name in the scientificName. Recommended best practice is to use a controlled vocabulary
nomenclaturalCode (occurrence) The nomenclatural code (or codes in the case of an ambiregnal name) under which the scientificName is constructed
decimalLatitude (occurrence) Value of decimal latitude (0.1 degree level: the resolution of locality data is shown roughly to prevent poaching)
decimalLongitude (occurrence) Value of decimal longitude (0.1 degree level: the resolution of locality data is shown roughly to prevent poaching)
geodeticDatum (occurrence) The ellipsoid, geodetic datum or spatial reference system (SRS) upon which the geographic coordinates given in decimalLatitude and decimalLongitude as based
coordinateUncertaintyInMeters (occurrence) The horizontal distance (in metres) from the given decimalLatitude and decimalLongitude describing the smallest circle containing the whole of the Location
verbatimCoordinateSystem (occurrence) The spatial coordinate system for the verbatimLatitude and verbatimLongitude or the verbatimCoordinates of the Location
islandGroup (occurrence) The name of the island group in which the Location occurs
island (occurrence) The name of the island on or near which the Location occurs
country (occurrence) The name of the country or major administrative unit in which the Location occurs
countryCode (occurrence) The standard code for the country in which the Location occurs
stateProvince (occurrence) The name of the next smaller administrative region than country (state, province, canton, department, region etc.) in which the Location occurs
locality (occurrence) The specific description of the place
individualCount (occurrence) The number of individuals represented present at the time of the Occurrence
establishmentMeans (occurrence) The process by which the biological individual(s) represented in the Occurrence became established at the location
preparations (occurrence) Preservation methods for a specimen
occurrenceID (multimedia) Occurrence ID
references (multimedia) An html webpage that shows the image or its metadata
title (multimedia) The media items title
type (multimedia) The kind of media object
format (multimedia) The format the image is exposed in
description (multimedia) A textual description of the content of the media item
created (multimedia) The date and time this media item was taken
creator (multimedia) The person who took the image, recorded the video or sound
audience (multimedia) A class or description for whom the image is intended or useful
rightsHolder (multimedia) A person or organisation owning or managing rights over the media item

Additional information

Note on the origin of Cristaria tenuis

We cannot conclude whether Cristaria tenuis is native or introduced based on our results (Fig. 6). However, it is notable that the sequences were all the same amongst the six samples from the two distant locales; C. tenuis might have been introduced in either Ishigaki Island or Fukuoka Prefecture.

“Metal artefacts” in CT data of thick-shell individuals

Several individuals, especially Inversiunio yanagawensis individuals, had significantly thicker shells; streak-like artefacts were generated at the surface of the shell as noise and were considered “metal artefacts” (e.g. Fig. 3a).

Acknowledgements

We thank Dr. Mallory Eckstut from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript. This paper includes partial study results funded by JSPS KAKENHI (Grant Number 18HP8029 and 15H02456), Open Call for Research and Development Proposal on River and Sabo Engineering Technologies (The Ministry of Land, Infrastructure, Transport and Tourism, Japan) and Kurita Water and Environment Foundation (18K010).

References

Supplementary material

Suppl. material 1: Elemental composition of the shell by EDS 
Authors:  Yuichi Kano
Data type:  csv