Biodiversity Data Journal :
Research Article
|
Corresponding author: Natalie Weber (natalieweber@gmx.de)
Academic editor: Ricardo Moratelli
Received: 21 Mar 2019 | Accepted: 23 May 2019 | Published: 18 Jun 2019
© 2019 Natalie Weber, Ricarda Wistuba, Jonas Astrin, Jan Decher
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Weber N, Wistuba R, Astrin J, Decher J (2019) New records of bats and terrestrial small mammals from the Seli River in Sierra Leone before the construction of a hydroelectric dam. Biodiversity Data Journal 7: e34754. https://doi.org/10.3897/BDJ.7.e34754
|
Sierra Leone is situated at the western edge of the Upper Guinean Forests in West Africa, a recognised biodiversity hotspot which is increasingly threatened by habitat degradation and loss through anthropogenic impacts. The small mammal fauna of Sierra Leone is poorly documented, although bats and rodents account for the majority of mammalian diversity. Based on morphological, genetic and echolocation data, we recorded 30 bat (Chiroptera), three shrew (Soricomorpha) and eleven rodent (Rodentia) species at the Seli River in the north of the country in 2014 and 2016, during a baseline study for the Bumbuna Phase II hydroelectric project. In 2016, 15 bat species were additionally documented at the western fringe of the Loma Mountains, a recently established national park and biodiversity offset for the Bumbuna Phase I dam. Three bat species were recorded for the first time in Sierra Leone, raising the total number for the country to 61. Further, two bat species are threatened and endemic to the Upper Guinean Forest and several taxa of small mammals are poorly known or represent undescribed species. Overall, the habitats of the project area supported a species-rich small mammal fauna including species of global conservation concern. Suitable mitigation measures and/or offsets are necessary to maintain biodiversity and ecosystems in a region that is under high human pressure.
Bumbuna Phase II hydroelectric project, Upper Guinean Forests, Chiroptera, Rodentia, Soricomorpha
Sierra Leone is situated at the western edge of the Upper Guinean Forest biodiversity hotspot in West Africa (
Within the Upper Guinean Forests, particularly the few (sub-)montane areas represent centres of species richness and are highly significant for the conservation of biodiversity (
This study provides new distribution data for bats and terrestrial small mammals from mountainous areas in northern Sierra Leone in a regional context. We present updates on the distribution of several range-restricted species and the national species list. Our data constitute the baseline to assess impacts on bats and terrestrial small mammals from the development of the Bumbuna Phase II hydroelectric dam, and to identify habitats and species of conservation concern for appropriate environmental management.
Bumbuna II is located along the Seli River, approximately 25 km south-southwest of Kabala, the capital of Koinadugu District (Fig.
Study area.
We sampled bats and terrestrial small mammals to cover all major habitat types in the study area (Table
Site IDs used in the text, full name, sampling year, coordinates and description of all study sites visited in the Bumbuna Phase II area (B) in the Northern Province, Sierra Leone, in 2014 and 2016 and at the western outskirts of the Loma Mountains National Park (Lo) in 2016.
Site ID |
Site Name |
2014 |
2016 |
Coordinates |
Description |
B1 |
Makarikari River 1 |
X |
X |
|
Riparian forest, steep and rocky ravine at seasonal tributary, bordering savannah and palm plantation. |
B2 |
Megboke River |
X |
|
Riparian forest at rocky tributary, farmbush with palm plantation, secondary forest on slope, village. |
|
B3 |
Pallama |
X |
X |
|
Hilly savannah, elephant grass, stony ground, xerophyte trees, patches of riparian forest and farmland. |
B4 |
Yogoron River 1 |
X |
X |
|
Mouth of Yogoron River, riparian forest, mangrove elements, sandbank with vegetation and rocks. |
B5 |
Makpoton River |
X |
|
Savannah, elephant grass on plateau, creeks, riparian forest, palm field. Degraded bare dry soil at shore. |
|
B6 |
Access road |
X |
|
Farmbush, steep slope, creek, young riparian forest; marsh areas, dead trees, sandy beach and bushes. |
|
B7 |
Yogoron River 2 |
X |
|
Riparian forest, agricultural and swamp lands; forested hills, artisanal gold mining. |
|
B8 |
Makarikari River 2 |
X |
|
Riparian forest, river with rocky steps, small sandy tributary. |
|
B9 |
Badala |
X |
|
Swamp area, peanut and rice fields, small river, bushy vegetation with tall elephant grass, village, forest patches. |
|
Ba |
Kamin Mata Cave |
X |
|
Accessible cave 10-15 m length, heavily degraded surroundings. |
|
Bb |
Yafarama Cave |
X |
|
Partly accessible cave, two openings, underneath plateau in hilly wood- and farmland. |
|
Lo1 |
Dorro River |
X |
|
Patches of forest, farmbush, cultivated and plantation land, swamp at river. |
|
Loa |
Sadia Konkoma Cave |
X |
|
Steep and rocky forested slope, several cavities, vegetation damaged by recent fire. |
We employed 12 m and 6 m mist nets (Ecotone, Poland: 2.8 m height, 5 shelves, 16 mm mesh, 2x70 dernier netting) set at both ground and canopy level and a three-bank harp trap (Austbat, FaunaTech, Australia) to capture bats, following international standard methods (
Captured bats were individually kept in cloth bags until processing. We took body mass and forearm length of all individuals and additional standard measurements of collected specimen (head and body, tail, ear, hind foot). Measurements were taken with a dial caliper (Holex 150/01, Germany) to the nearest 0.1 mm. Body mass was measured with spring balances (Pesola 50 g, 100 g, 600 g, Switzerland). Sex, age class and reproductive state were determined visually. Echolocation calls of representatives of hipposiderid and rhinolophid bats were recorded with a Pettersson D240x bat detector in 10x time expansion mode and transferred to a H2 digital sound recorder (www.zoom.co.jp). The constant-frequency (cf) component of echolocation calls was measured to aid identification of problematic taxa, as it is highly specific in many hipposiderid and rhinolophid bats. Most calls were obtained from hand-held bats and on one occasion of bats in flight in a cave. Calls were later analysed with the software BatSound (Version 1.3.3). A total of 18 voucher specimens of species not previously recorded in the area or country was preserved in 70% ethanol for documentation and tissue samples were preserved in 95% ethanol. Additional genetic samples were collected from the patagium of some released individuals. Specimens and tissue samples are deposited in the research collection of Jakob Fahr (RCJF; Division of Evolutionary Biology, TU Braunschweig, Germany). Collection numbers have not yet been assigned, but collection plus field number represents a unique identifier. In addition to published records of bats from Sierra Leone, the exhaustive database AfriBats (http://afribats.myspecies.info) provided context for our results, including museum records from: National History Museum, London (BMNH); Musée Royal de l’Afrique Centrale, Tervuren (MRAC); Royal Ontario Museum, Toronto (ROM); US National Museum, Washington D.C. (USNM).
For terrestrial small mammals, each of six sites was sampled for one to three consecutive nights. We mainly used Sherman live traps (LFA Life Trap, H.P. Sherman Traps, Inc.). Some Victor Metal Pedal Rat snap traps were set out in places difficult to access, e.g. on tree branches and vines, rock outcrops or in creek beds and to capture trap-shy species. At each site, one or two pitfall lines of 40 metres of plastic drift fence with four or five buckets were installed. Four Tomahawk live traps (203 Collapsible Squirrel Life Trap, Tomahawk Live Traps) were used opportunistically to target larger species. Palm fruit, peanut butter with oats and cat food was used as bait. Visual estimations of the microhabitat (canopy and ground cover, see Suppl. material
Survey techniques complied with international standard methods for measuring and monitoring small mammal diversity (
The Hipposideros ruber-caffer species complex (Chiroptera: Hipposideridae) comprises several distinct lineages, which likely represent cryptic bat species (
Maximum likelihood tree showing phylogenetic relationship of Hipposideros bats (in particular within the H. aff. ruber species complex) from the Bumbuna Phase II and I areas, and from other localities in Africa (
We used genetic distances as a mean to verify identification of collected terrestrial small mammals. To that end, we obtained 35 tissue samples (mostly liver) for DNA analysis. Laboratory protocol and DNA sample deposition are identical to those stated above. For PCR of the cytochrome oxidase 1 (CO1) gene (DNA barcoding fragment, 658 bp), we combined the primers LCO1490-JJ and HCO2198-JJ (
Smoothed species accumulation curves were generated with the programme EstimateS (Version 9.1;
Voucher specimens
Bumbuna II: Rhinolophus landeri (RCJF-NW2811); Hipposideros marisae (RCJF-NW2812); Macronycteris vittatus (RCJF-NW3664); Nycteris macrotis (RCJF-NW2823); Nycteris thebaica (RCJF-NW2808); Coleura afra (RCJF-NW3790); Pipistrellus nanulus (RCJF-NW2891, RCJF-NW2904); Scotoecus hirundo (RCJF-NW2900); Scotophilus viridis (RCJF-NW2855); Scotophilus dinganii (RCJF-NW2859); Glauconycteris poensis (RCJF-NW2866); Mops condylurus (RCJF-NW2851). Loma: Scotophilus nux (RCJF-NW3697); Chaerephon nigeriae (RCJF-NW3682); Mops nanulus (RCJF-NW3645); Mops thersites (RCJF-NW3662); Mops trevori (RCJF-NW3646).
DNA voucher and GenBank accession numbers
Bumbuna II: Hipposideros aff. ruber C1 (ZFMK-DNA-FC19476444, MH713747; ZFMK-DNA-FC19476428, MH713749; ZFMK-DNA-FC19476460, MH713750; ZFMK-DNA-FC19476452, MH713751); Hipposideros aff. ruber D(1) (ZFMK-DNA-FC19476467, MH713740; ZFMK-DNA-FC19476396, MH713741; ZFMK-DNA-FC19476404, MH713743; ZFMK-DNA-FC19476412, MH713744; ZFMK-DNA-FC19476420, MH713745; ZFMK-DNA-FC19476468, MH713748); Hipposideros aff. ruber D(?) (ZFMK-DNA-FC19476380, MH713742). Loma: Hipposideros aff. ruber D(?) (ZFMK-DNA-FC19476436, MH713746).
Voucher specimens
Bumbuna II: Crocidura olivieri (ZFMK-MAM-2014.0601); Crocidura cf. theresae (ZFMK-MAM-2014.0603); Crocidura sp.1 (ZFMK-MAM-2014.0602); Cricetomys gambianus (ZFMK-MAM-2014.0641; ZFMK-MAM-2014.0642; ZFMK-MAM-2014.0643); Gerbilliscus kempi (ZFMK-MAM-2014.0604); Lophuromys sikapusi (ZFMK-MAM-2014.0610); Uranomys ruddi (ZFMK-MAM-2014.0640); Hylomyscus simus (ZFMK-MAM-2014.0614); Lemniscomys striatus (ZFMK-MAM-2014.0607‒0609; ZFMK-MAM-2014.0638‒0639; ZFMK-MAM-2014.0644); Malacomys edwardsi (ZFMK-MAM-2014.0611‒0612); Mastomys erythroleucus (ZFMK-MAM-2014.0632; ZFMK-MAM-2014.0634‒0636; ZFMK-MAM-2014.0646; ZFMK-MAM-2014.0685); Mus musculoides/minutoides (ZFMK-MAM-2014.0613); Mus setulosus (ZFMK-MAM-2014.0615‒0616); Praomys rostratus (ZFMK-MAM-2014.0605‒0606; ZFMK-MAM-2014.0617‒0631; ZFMK-MAM-2014.0633; ZFMK-MAM-2014.0637; ZFMK-MAM-2014.0645).
DNA voucher and GenBank accession numbers
Bumbuna II: Crocidura olivieri (ZFMK-TIS-24165, ZFMK-DNA-0171606048, MH713719); Crocidura cf. theresae (ZFMK-TIS-26117; ZFMK-DNA-0171661915; MH713734); Crocidura sp.1 (ZFMK-TIS-24084, ZFMK-DNA-0171606069, MH713711); Cricetomys gambianus (ZFMK-TIS-24076, ZFMK-DNA-0171606071, MH713709; ZFMK-TIS-26466, ZFMK-DNA-0171661910, MH713739); Gerbilliscus kempi (ZFMK-TIS-26099, ZFMK-DNA-0171606057, MH713727); Lophuromys sikapusi (ZFMK-TIS-26115, ZFMK-DNA-0171661916, MH713733); Uranomys ruddi (ZFMK-TIS-26464, ZFMK-DNA-0171661911, MH713738); Hylomyscus simus (ZFMK-TIS-26101, ZFMK-DNA-0171606058, MH713728); Lemniscomys striatus (ZFMK-TIS-24177, ZFMK-DNA-0171606052, MH713722; ZFMK-TIS-24187, ZFMK-DNA-0171606054, MH713724; ZFMK-TIS-26103, ZFMK-DNA-0171606059, MH713729; ZFMK-TIS-26462, ZFMK-DNA-0171661912, MH713737); Mastomys erythroleucus (ZFMK-TIS-24154, ZFMK-DNA-0171606064, MH713716; ZFMK-TIS-24167, ZFMK-DNA-0171606049, MH713720; ZFMK-TIS-24185, ZFMK-DNA-0171606053, MH713723; ZFMK-TIS-26105, ZFMK-DNA-0171661919, MH713730; ZFMK-TIS-26111, ZFMK-DNA-0171661918, MH713731; ZFMK-TIS-26113, ZFMK-DNA-0171661917, MH713732); Mus setulosus (ZFMK-TIS-24148, ZFMK-DNA-0171606066, MH713714; ZFMK-TIS-26362, ZFMK-DNA-0171661914, MH713735); Praomys rostratus (ZFMK-TIS-24088, ZFMK-DNA-0171606068, MH713712; ZFMK-TIS-24144, ZFMK-DNA-0171606067, MH713713; ZFMK-TIS-24150, ZFMK-DNA-0171606065, MH713715; ZFMK-TIS-24156, ZFMK-DNA-0171606063, MH713717; ZFMK-TIS-24160, ZFMK-DNA-0171606061, MH713718; ZFMK-TIS-24169, ZFMK-DNA-0171606050, MH713721; ZFMK-TIS-26095, ZFMK-DNA-0171606055; ZFMK-TIS-26097, ZFMK-DNA-0171606056, MH713726; ZFMK-TIS-24080, ZFMK-DNA-0171606070, MH713710).
In total, we captured 352 bats (Bumbuna II: 268, Loma: 84) in 34 species (Bumbuna II: 29, Loma: 14) and eight families (Tables
Bat species recorded in the Bumbuna Phase II area and west of the Loma Mountains National Park (Loma) during this study, in the Bumbuna Phase I area (
Family or (sub-)order / Species |
BII 2016 |
BII 2014 |
BI 2006 |
Loma 2016 |
Previous references |
RL |
Habitat |
Cave |
Record |
Pteropodidae |
|||||||||
Micropteropus pusillus |
X |
X |
X |
|
S |
||||
Epomops buettikoferi |
X |
X |
X |
X |
F(S) |
||||
Eidolon helvum |
X |
X |
NT |
FS |
1st B |
||||
Hypsignathus monstrosus |
X |
X* |
|
F(S) |
1st B |
||||
Rousettus aegyptiacus |
X |
X |
X |
FS |
+ |
||||
Myonycteris leptodon |
X |
X |
X |
F(S) |
|||||
Myonycteris angolensis |
X |
X |
F(S) |
(+) |
1st B |
||||
Nanonycteris veldkampii |
X |
X |
X |
F(S) |
|||||
Rhinolophidae |
|||||||||
Rhinolophus landeri |
X |
|
(F)S |
+ |
1st B |
||||
Rhinolophus guineensis |
X |
X |
VU |
F |
+ |
||||
Rhinolophus fumigatus |
X* |
X |
S |
+ |
|||||
Rhinolophus denti knorri |
X |
S |
+ |
||||||
Rhinolophus ziama |
X |
EN |
F |
+ |
|||||
Hipposideridae |
|||||||||
Hipposideros marisae |
X |
VU |
F |
+ |
1st SL |
||||
Hipposideros aff. ruber C11 |
X |
? |
X |
n.a. |
FS |
(+) |
|||
Hipposideros aff. ruber D1 |
X |
X |
X |
X |
n.a. |
FS |
(+) |
||
Hipposideros jonesi |
X |
NT |
FS |
+ |
|||||
Hipposideros abae |
X |
X |
S |
+ |
|||||
Macronycteris vittatus 2 |
X |
? |
X |
|
NT |
S |
(+) |
2nd SL |
|
Doryrhina cyclops 2 |
X |
X |
F(S) |
||||||
Nycteridae |
|||||||||
Nycteris arge |
X |
X |
F |
||||||
Nycteris grandis |
X |
X |
F |
||||||
Nycteris macrotis |
X |
FS |
(+) |
1st B |
|||||
Nycteris thebaica |
X |
X |
S |
+ |
1st B |
||||
Nycteris hispida |
X |
|
FS |
||||||
Emballonuridae |
|||||||||
Coleura afra |
X |
S |
+ |
1st SL |
|||||
Miniopteridae |
|||||||||
Miniopterus [schreibersii] villiersi |
X |
n.a. |
F(S) |
+ |
|||||
Vespertilionidae |
|||||||||
Myotis bocagii |
X |
X |
X |
F(S) |
|||||
Pipistrellus inexspectatus |
|
S |
|||||||
Pipistrellus nanulus |
X |
F(S) |
1st B |
||||||
Neoromicia somalica |
USNM 462850 |
S |
|||||||
Neoromicia sp. nov. [aff. nana] |
X |
n.a. |
FS |
||||||
Scotoecus hirundo |
X |
S |
2nd SL |
||||||
Scotophilus viridis |
X |
S |
1st B |
||||||
Scotophilus nux |
X |
F |
|||||||
Scotophilus dinganii |
X |
X |
S |
1st B |
|||||
Glauconycteris poensis |
X |
X |
X |
F |
1st B |
||||
Molossidae |
|||||||||
Chaerephon nigeriae |
X |
S |
2nd SL |
||||||
Mops condylurus |
X |
X |
X |
USNM 462857 |
S |
||||
Mops nanulus |
X |
F |
|||||||
Mops thersites |
X |
F |
|||||||
Mops trevori |
X |
DD |
(F)S |
1st SL |
|||||
Species total |
18/19* |
20 |
19 |
14/15* |
|||||
Species total BII |
30 |
||||||||
Species total BI and II, Loma and wider area |
42 |
||||||||
Soricomorpha |
|||||||||
Crocidura jouvenetae |
X |
F |
|||||||
Crocidura nigeriae |
X |
FS |
|||||||
Crocidura olivieri |
X |
|
FS |
1st B |
|||||
Crocidura poensis |
X |
|
FS |
||||||
Crocidura cf. theresae |
X |
|
FS |
1st B | |||||
Crocidura sp.1 |
X |
1st B |
|||||||
Rodentia |
|||||||||
Cricetomys emini |
X |
|
F |
||||||
Cricetomys gambianus |
X |
|
S |
1st B |
|||||
Gerbilliscus kempi |
X |
|
S |
1st B |
|||||
Hybomys planifrons |
X |
F |
|||||||
Hylomyscus simus |
X |
X |
F |
||||||
Lemniscomys striatus |
X |
X |
|
S |
|||||
Lophuromys sikapusi |
X |
X |
|
FS |
|||||
Malacomys edwardsi |
X |
X |
F |
||||||
Mastomys erythroleucus |
X |
X |
|
S |
|||||
Mastomys natalensis 3 |
|
S |
|||||||
Mus musculus |
|
||||||||
Mus musculoides/ minutoides |
X |
X |
|
S |
|||||
Mus setulosus |
X |
X |
|
FS |
|||||
Praomys rostratus |
X |
X |
|
F |
|||||
Rattus rattus |
|
||||||||
Uranomys ruddi |
X |
|
S |
1st B |
|||||
Species total |
14 |
13 |
|||||||
Species total BI and II and wider area |
22 |
Bats recorded at eight study sites and two caves in the Bumbuna Phase II area (B) in 2014 and 2016, and at the western outskirts of the Loma Mountains National Park (Lo) in 2016. Study sites in italics were sampled in 2014 and 2016. *: only observed, not captured. Species totals treat Hipposideros aff. ruber as one species. 1
Family / Species | B1 | B2 | B3 | B4 | B5 | B6 | B7 | B8 | Ba | Bb | Lo1 | Loa | Total |
Pteropodidae | |||||||||||||
Micropteropus pusillus |
10 | 4 | 7 | 6 | 27 | ||||||||
Epomops buettikoferi | X* | X* | 5 | 18 | 2 | 12 | 8 | 4 | 49 | ||||
Eidolon helvum | 2 | 2 | 4 | ||||||||||
Hypsignathus monstrosus | 1 | X* | 1 | ||||||||||
Rousettus aegyptiacus | 1 | 4 | X* | 12 | X* | 17 | |||||||
Myonycteris leptodon | 1 | 1 | 4 | 6 | |||||||||
Myonycteris angolensis | 1 | 5 | 6 | ||||||||||
Nanonycteris veldkampii | 1 | 13 | 28 | 8 | 44 | 94 | |||||||
Rhinolophidae | |||||||||||||
Rhinolophus landeri | 2 | 2 | |||||||||||
Rhinolophus guineensis | 1 | 1 | |||||||||||
Rhinolophus fumigatus | X* | - | |||||||||||
Hipposideridae | |||||||||||||
Hipposideros marisae | 5 | 1 | 6 | ||||||||||
Hipposideros aff. ruber | 44 | 3 | 1 | 4 | 12 | 3 | 1 | 2 | X* | 70 | |||
Hipposideros aff. ruber C11 | 1 | 1 | 1 | 1 | 4 | ||||||||
Hipposideros aff. ruber D1 | 2 | 1 | 2 | 1 | 1 | 1 | 8 | ||||||
Hipposideros abae | 2 | 2 | 4 | ||||||||||
Macronycteris vittatus 2 |
1 | 1 | 2 | ||||||||||
Doryrhina cyclops 2 | 3 | 1 | 4 | ||||||||||
Nycteridae | |||||||||||||
Nycteris arge | 1 | 1 | |||||||||||
Nycteris grandis | 1 | 1 | |||||||||||
Nycteris macrotis | 1 | 1 | 2 | ||||||||||
Nycteris thebaica | 3 | 3 | |||||||||||
Nycteris hispida | 2 | 2 | |||||||||||
Emballonuridae | |||||||||||||
Coleura afra | 1 | 1 | |||||||||||
Vespertilionidae | |||||||||||||
Myotis bocagii | 1 | 5 | 1 | 7 | |||||||||
Pipistrellus nanulus | 1 | 1 | 2 | ||||||||||
Scotoecus hirundo | 1 | 1 | |||||||||||
Scotophilus viridis | 1 | 1 | |||||||||||
Scotophilus nux | 1 | 1 | |||||||||||
Scotophilus dinganii | 1 | 1 | 2 | ||||||||||
Glauconycteris poensis | 4 | 1 | 1 | 6 | |||||||||
Molossidae | |||||||||||||
Chaerephon nigeriae | 1 | 1 | |||||||||||
Mops condylurus | 3 | 1 | 3 | 7 | |||||||||
Mops nanulus | 6 | 6 | |||||||||||
Mops thersites | 1 | 1 | |||||||||||
Mops trevori | 2 | 2 | |||||||||||
Total | 60 | 9 | 26 | 46 | 20 | 11 | 56 | 36 | 4 | - | 84 | - | 352 |
Number of species |
7 +1* |
3 +1* |
11 | 8 | 4 | 6 | 8 | 10 | 2 | 3* |
14 +1* |
1* | 34 |
In 1,423 trap nights, 106 individuals belonging to 14 species, comprising three shrew and eleven rodent species, were captured (Tables
Terrestrial small mammals recorded at six study sites in the Bumbuna Phase II area (B) in 2014, and capture effort and success.
(Sub-)Order / Species |
B1 |
B2 |
B3 |
B5 |
B6 |
B9 |
Total |
Soricomorpha |
|||||||
Crocidura olivieri |
1 |
1 |
|||||
Crocidura cf. theresae |
1 |
1 |
|||||
Crocidura sp.1 |
1 |
1 |
|||||
Rodentia |
|||||||
Cricetomys gambianus |
1 |
1 |
1 |
2 |
2 |
7 |
|
Gerbilliscus kempi |
1 |
1 |
|||||
Hylomyscus simus |
1 |
1 |
|||||
Lemniscomys striatus |
2 |
1 |
4 |
7 |
|||
Lophuromys sikapusi |
1 |
1 |
|||||
Malacomys edwardsi |
1 |
1 |
2 |
||||
Mastomys erythroleucus |
2 |
1 |
1 |
3 |
7 |
||
Mus musculoide/minutoides |
1 |
1 |
|||||
Mus setulosus |
1 |
3 |
4 |
||||
Praomys rostratus |
40 |
16 |
4 |
9 |
2 |
71 |
|
Uranomys ruddi |
1 |
1 |
|||||
Total |
44 |
21 |
7 |
5 |
15 |
14 |
106 |
Number of species |
5 |
5 |
3 |
5 |
4 |
7 |
14 |
Number of traps |
115 |
122 |
122 |
72 |
89 |
122 |
|
Trap nights |
345 |
244 |
244 |
72 |
152 |
366 |
1423 |
Trap success (%) |
12.8 |
8.6 |
2.9 |
6.9 |
9.9 |
3.8 |
7.4 |
In the following species accounts, we present information on systematics, distribution and conservation status of new species records for the wider Bumbuna area and Loma in 2014 and 2016. For species already documented from Bumbuna I (
Order Chiroptera
Family Pteropodidae
Micropteropus pusillus (Peters, 1868) Peters's Dwarf Epauletted Fruit Bat
Eight individuals were captured in Bumbuna II in savannah habitats in 2014 (B3), and 19 individuals at the same site and along rivers in 2016 (B3, B7, B8). Out of the 27 individuals, 17 were females. Two females were pregnant (30 + 31 Mar) and five were lactating (6 + 7 Apr, 28 May).
Epomops buettikoferi (Matschie, 1899) Buettikofer's Epauletted Fruit Bat
With 49 individuals (Bumbuna II: 45, Loma: 4), this was the second most frequently encountered fruit bat species in our study. In Bumbuna II, the species was found at five sites (B3, B4, B6, B7, B8) in savannah and riparian forest habitats in varying condition. We captured 19 females, including one pregnant (30 Mar) and six lactating (24, 30, 31 Mar, 1 + 7 Apr) females.
Eidolon helvum (Kerr, 1792) African Straw-coloured Fruit Bat
Four individuals of this species were captured, two in Bumbuna II (B7) and two in Loma, representing new records for both areas. All four individuals were encountered between agricultural lands and riparian forests. Eidolon helvum is known from several other localities, mostly day roosts, across the country (
Hypsignathus monstrosus H. Allen, 1861 Hammer-headed Fruit Bat
Male individuals of this largest African bat were heard calling in Loma and one sub-adult female was captured with a canopy net in Bumbuna II in 2016 (B7). Hypsignathus monstrosus was known from the Loma Mountains prior to this study (
Rousettus aegyptiacus (E. Geoffroy, 1810) Egyptian Fruit Bat
We captured 17 individuals of the Egyptian fruit bat in our study in 2016 (B4, B8: 5, Lo1: 12). One of eight females was lactating (7 Apr). We further observed a few individuals of this fruit bat at the entrance of a cavity in Sadia Konkoma (Loa) by day. A local guide reported that the majority of individuals disappeared shortly before our visit, probably due to a recent fire which burnt much of the vegetation on the rocky slope. Another approximately 60 individuals were counted in Yafarama cave (Bb). The species is hunted for bushmeat at day roosts in the area.
Myonycteris leptodon K. Andersen, 1908 Sierra Leone Collared Fruit Bat
We encountered six individuals of M. leptodon at three sites in Bumbuna II (B1, B3, B8). Three of them were females, including one which was lactating (7 Apr).
Myonycteris angolensis smithii (Thomas, 1908) Angolan Fruit Bat
Five females of this fruit bat were recorded in Loma and one male in hilly woodland savannah in Bumbuna II (B3). Two females were lactating (25 Mar). The few previous records from Sierra Leone are patchily distributed, and include the type specimen for this taxon (
Nanonycteris veldkampii (Jentink, 1888) Veldkamp's Epauletted Fruit Bat
With a total of 94 individuals, this species constituted the most numerous species in our study, all of them recorded in 2016. Veldkamp's bat was common in Loma (n = 44 individuals) and occurred at four sites in Bumbuna II (n = 50 individuals; B3, B4, B7, B8). Females were represented by 47 individuals, 28 being adult. Out of these, five were pregnant (25, 30, 31 Mar, 2 Apr) and eleven lactating (25, 27, 30, 31 Mar, 1, 3, 7, 8 Apr). The species was likely absent from the study area in 2014.
Family Rhinolophidae
Rhinolophus landeri Martin, 1838 Lander's Horseshoe Bat
Two males of R. landeri were captured on rocky slopes (B1) in 2014. Forearm length (43.4-43.9 mm) and cf frequency were in the typical range of this species (Table
Echolocation calls of rhinolophid and hipposiderid bats recorded in the Bumbuna Phase II area 2014 and 2016, and at the western outskirts of the Loma Mountains National Park in 2016. Mean cf frequencies are given with standard deviation (SD). Min / Max: minimum / maximum frequency, n: sample size, M: male, F: female.
Species |
Mean±1 SD [kHz] |
Min–Max [kHz] |
n |
Sex |
Rhinolophus landeri |
102.0 |
101.5‒102.5 |
2 |
M |
Rhinolophus guineensis |
82.0 |
1 |
F |
|
Hipposideros marisae |
142.5 |
141.0‒144.0 |
2 |
F |
Hipposideros marisae |
146.5 |
146.0‒147.0 |
2 |
M |
Hipposideros aff. ruber C1 |
145.7±4.5 |
142.0‒152.0 |
3 |
M/F |
Hipposideros aff. ruber D |
122.5±2.4 |
120.0‒125.0 |
4 |
F |
Hipposideros aff. ruber D |
127.0 |
2 |
M |
|
Hipposideros abae |
103.0 |
1 |
F |
|
Macronycteris vittatus |
62.5 |
62.0‒63.0 |
2 |
M/F |
Doryrhina cyclops |
54.5 |
54.0‒55.0 |
2 |
F |
Rhinolophus fumigatus (in flight, in Bb) |
51.5 |
1 |
Rhinolophus guineensis Eisentraut, 1960 Guinean Horseshoe Bat
One pregnant female was recorded in riparian forest at the Yogoron River (B7) in 2016 (30 Mar). Forearm length (46.8 mm) and echolocation frequency of this individual fit with previous observations (Table
Rhinolophus fumigatus Sanborn, 1939 Rüppell's Horseshoe Bat
This horseshoe bat was tentatively identified by echolocation recordings from Yafarama cave (Bb; Table
Family Hipposideridae
Hipposideros marisae Aellen, 1954 Aellen’s Leaf-nosed Bat
In 2014, five individuals of this small leaf-nosed bat (Fig.
This constitutes the first record of H. marisae for Sierra Leone and only the ninth observation of this species in total, with seven records dating back to at least 1990 (
Hipposideros aff. ruber (Noack, 1893) Noack's Roundleaf Bat
Hipposideros aff. ruber belongs to the wider H. caffer/ruber species complex, which shows a high level of cryptic diversity based on Cyt-b (
Hipposideros aff. ruber C1 was represented by five samples in the genetic sequencing (Fig.
Genetic sequencing revealed that 13 samples belonged to Hipposideros aff. ruber D. Eight of these bats were from six sites visited in this study (B1, B2, B4, B5, B6, Lo1). One of four females was pregnant (24 Mar) and one was lactating (2 Apr). Echolocation call frequencies of two males were higher than the frequencies used by four females (Table
Hipposideros abae J.A. Allen, 1917 Aba Leaf-nosed Bat
We captured two females of the Aba roundleaf bat at Kamin Mata (Ba) during the day, one was lactating (24 May). At the time of visit, the cave harboured a colony of roughly 100 individuals of this species and around 300 individuals of Hipposideros aff. ruber. Two males were caught around the transmission line (B5) near Kamin Mata, just on the other side of Seli River. The cf frequency of one female (Table
Macronycteris vittatus (Peters, 1852) Striped Leaf-nosed Bat
Macronycteris has long been considered a synonym of Hipposideros. We follow
We recorded two individuals of this large hipposiderid in 2016, one pregnant female (26 Mar; Fig.
Doryrhina cyclops (Peters 1871) Cyclops Leaf-nosed Bat
We follow
We captured four individuals of Cyclops leaf-nosed bat in 2014, two males and two females which were both lactating (26 + 28 May). One female was encountered at B3, the other three individuals were captured when emerging from their day roost, a hollow tree on a freshly burnt field at B2. Echolocation calls of the two females were recorded and frequencies were in the common range of D. cyclops (Table
Family Nycteridae
Nycteris arge Thomas, 1903 Bates's Slit-faced Bat
One male was captured in riparian forest at B1.
Nycteris grandis Peters, 1865 Large Slit-faced Bat
One male of the large slit-faced bat was recorded in wooded savannah at B6, representing the third record for Sierra Leone (
Nycteris macrotis Dobson, 1876 Large-eared Slit-faced Bat
We captured two males of this species, one in forest at B1 and one in degraded riparian forest at the Seli River (B4). The large-eared slit-faced bat is a widespread species, which has been previously documented from several other localities in Sierra Leone (
Nycteris thebaica E. Geoffroy, 1818 Egyptian Slit-faced Bat
Three individuals of N. thebaica were recorded in Bumbuna II, all in riparian forest at the cave system at B1, one male and one female in 2014, and one sub-adult female in 2016. Nycteris thebaica is difficult to distinguish from N. gambiensis, but the latter is smaller than N. thebaica in West Africa (
Nycteris hispida (Schreber, 1775) Hairy Slit-faced Bat
Four individuals were found roosting in an open cavity under a large rock in a partly dried out riverbed near B2 during the day. One adult and one sub-adult flew off, but one lactating female (25 May) was captured with a young attached. Nycteris hispida is known from other locations in the Northern Province (
Family Emballonuridae
Coleura afra (Peters, 1852) African Sheath-tailed Bat
We captured one male of this emballonurid bat in riparian forest at B4 in 2016 (Fig.
Family Vespertilionidae
Myotis bocagii (Peters, 1870) Rufous Mouse-eared Bat
A total of seven individuals was obtained in both study periods. Six records were from the Seli River (B5, B6) and one individual was captured over Makarikari River (B8). The single female was pregnant (4 Jun).
Pipistrellus nanulus Thomas, 1904 Tiny Pipistrelle
We recorded one male and one female in 2014 (B4, B6). The tiny pipistrelle was previously documented from four localities in the country (
Scotoecus hirundo (de Winton, 1899) Dark-winged Lesser House Bat
Our capture represents the second record of S. hirundo for Sierra Leone, the first is from Musaia approximately 50 km north of our locality (
Scotophilus viridis (Peters, 1852) Green House Bat
We captured one male in the savannah landscape at B3.
Scotophilus nux Thomas, 1904 Nut-coloured Yellow Bat
One male was captured over a swamp at the forest edge in Loma. The nut-coloured yellow bat has been previously recorded from the rainforest zone in southern Sierra Leone. Our record constitutes the northernmost locality of this species to date, with the nearest record from 10 miles north of Panguma, 100 km to the south of Loma (
Scotophilus dinganii (A. Smith, 1833) Yellow-bellied House Bat
Our samples included two pregnant females (7 Apr, 29 May). We encountered one bat in woodland savannah (B3), and one over the Makarikari River (B8). The yellow-bellied house bat was previously known from three localities in Sierra Leone, Bonthe (
Glauconycteris poensis (Gray, 1842) Abo Butterfly Bat
We recorded a total of six males of this species in both study periods and areas. Four individuals were captured at B5 in 2014 after emergence from their day roost. One individual was caught over a small river at B7 and one individual was from Loma. Glauconycteris poensis is known from several localities in the south of Sierra Leone (
Family Molossidae
Chaerephon nigeriae nigeriae Thomas, 1913 Nigerian Free-tailed Bat
We captured one nulliparous female in Loma, which constitutes the second record of this savannah species for Sierra Leone. The first record is from east of Fintonia in Outamba-Kilimi National Park (
Mops condylurus (A. Smith, 1833) Angolan Free-tailed Bat
Seven individuals of this molossid bat were captured with canopy nets, three in savannah habitats (B3), one over Makarikari River (B8) and three in Loma. There are several previous records of M. condylurus from Sierra Leone (
Mops nanulus J. A. Allen, 1917 Dwarf Free-tailed Bat
This very small molossid was only encountered in Loma. Of the six individuals, four were females, one being pregnant (26 Mar). Our record is the third of M. nanulus for the country (
Mops thersites (Thomas, 1903) Railer Free-tailed Bat
A single female of this species was recorded in Loma. Mops thersites was previously documented from several localities in the southern half of the country (
Mops trevori J.A. Allen, 1917 Trevor's Free-tailed Bat
We recorded two females of this rarely observed bat in Loma, one being pregnant (25 Mar; Fig.
Order Soricomorpha
Family Soricidae
Crocidura olivieri (Lesson, 1827) Olivier’s Shrew, African Giant Shrew
One individual of this large common shrew was captured in a pitfall trap close to the Seli River at B2. The species is found in a wide variety of habitats including forest, savannah, degraded forest, farmbush, shrubland and forest clearings in most of sub-Saharan Africa (
Crocidura cf. theresae (Heim de Balsac, 1968) Therese’s Shrew
One male was captured in a pitfall trap at B9. This species was tentatively assigned to C. theresae based on morphological characteristics (head-body length: 89.0 mm, tail: 62.0 mm, hind foot: 15.0 mm, ear: 6.9 mm, body mass: 14.5 g). Molecular analyses supported another position within the C. poensis species group, C. grandiceps (
Crocidura sp.1
One pregnant female of this shrew was captured at B1 (22 May). This individual also belongs to the C. poensis species complex. Our specimen shares morphological characteristics with C. longipes (head-body length: 93.0 mm, tail: 59.0 mm, hind foot: 15.3 mm, ear: 7.3 mm, body mass: 19.0 g), but the genetic identification is not clear and requires further information and reference sequences. Our individual was captured in microhabitat with 75% canopy cover. The C. poensis species complex comprises large-sized species that are distributed throughout the Guinea-Congolian rainforests and savannahs. Taxonomic relationships within this group are currently not resolved and a revision based on morphological and genetic studies is urgently needed.
Order Rodentia
Family Nesomyidae
Cricetomys gambianus (Wroughton, 1910) Gambian Giant Pouched Rat, Giant Rat
Seven individuals (2 males, 4 females, 1 unspecified) of this large rodent were captured in Tomahawk traps at all sites except for B3. All but two individuals were caught in forest vegetation, one female on a branch at about 2 m height. One female was lactating (28 May). Three voucher specimens were identified as C. gambianus based on morphological and molecular analyses. The species occurs in grassland, woodland and anthropogenic habitats in the northern savannahs of West and Central Africa (
Family Muridae
Gerbilliscus kempi (Wroughton, 1906) Kemp's or Northern Savannah Gerbil
One male of this common West and Central African gerbil was obtained at B5 in savannah with elephant grass. This species can be distinguished from the less common G. guineae, which is restricted to northern Sierra Leone, by its shorter, untufted tail (
Lophuromys sikapusi (Temminck, 1853) Rusty-bellied Brush-furred Rat
One male of this distinctive orange-bellied rat was captured in a valley with fields at B9 in a pitfall trap set in dense tall elephant grass. This individual was captured in microhabitat with no canopy cover.
Uranomys ruddi (Dollmann, 1909) Rudd's Brush-furred Mouse
Only one female of this distinctive savannah and open woodland mouse was caught at the edge of a field at B9. The species is very rare in surveys of small mammals (
Hylomyscus simus (Allen and Coolidge, 1930) West African Wood Mouse
One male of this arboreal species was caught on a branch in young forest at B5. We follow
Lemniscomys striatus (Linnaeus, 1758) Typical Striped Grass Mouse
Seven individuals (2 male, 2 female, 3 unspecified) of this striped grass mouse were caught at three sites, mostly in savannahs with elephant grass (B5, B9) or termite mounds (B3). One female was pregnant with 4 embryos (30 May). Following
Malacomys edwardsi (Rochebrunne, 1885) Edward's Swamp Rat
One male was caught on a steep hill in gallery forest at B1 and one pregnant female with two embryos in young forest near the Seli River at B6. Both individuals were captured in microhabitat with an average of 93.5% canopy cover.
Mastomys erythroleucus (Temminck, 1853) Multimammate Mouse
Seven specimens of this common rodent were captured at all study sites except for B1 and B9. Two males were captured at B2 in a rice patch near a palm oil cooking site, one male at B3 in elephant grass savannah, three (two females, one unspecified) at B6 in grassland in a marshy area near the shore of the Seli River and one male at B5. Our specimens belong to the West African phylogroup A (
Mus musculoides (Temminck, 1853) / M. minutoides (Smith, 1834) Pigmy Mice
One male belonging to this species complex was captured at B2. It is distinguished from M. setulosus based on its small size. This tiny mouse was captured in microhabitat with 40.0% canopy cover.
Mus setulosus (Peters, 1876) Peter's Pygmy Mouse
One male of this relatively large pygmy mouse species was captured at B1 in riparian forest and three males at B9 in elephant grass and shrubs. All individuals were captured in microhabitat with an average of 25.0% canopy cover.
Praomys rostratus (Miller, 1900) West African Soft-furred Mouse
We captured 71 individuals (21 male, 36 female, 14 unspecified) of this common forest rat. It was the most common rodent species recorded in our study and captured at almost all study sites (B1, B2, B3, B6, B9), almost exclusively in forest habitats. Seven females were pregnant (one with two embryos, 24 May; two with three embryos, 23 + 27 May). The microhabitat of 68 captured individuals had an average of 73.4% canopy cover.
Family Sciuridae
Paraxerus poensis (Smith, 1834) Green Bush Squirrel
One individual was photographed on 3 June by ornithologist Paul Robinson at B3. This squirrel is common in forest edge, secondary forest and farmbush habitats. It is widely distributed in the Upper Guinean Forests of West Africa to the River Volta and in the Lower Guinean Forests from the Niger River eastwards into Central Africa (
Euxerus erythropus (Geoffroy, 1803) Striped Ground Squirrel
Observed but not photographed crossing the road at S3 on 20 May.
Our study shows that Bumbuna II and its surroundings harbour a species-rich small mammal fauna and highlights the relevance of biodiversity surveys in understudied areas for impact assessments prior to major development projects. We documented 30 bat species from Bumbuna II, half of which were not previously known from the wider area (Table
The species accumulation curves for bats rise continuously, suggesting that additional species can be expected (Fig.
We raised the number of bat species documented from Sierra Leone from 58 to 61. The 30 bat species observed in Bumbuna II within an area of 82 km2 represent almost half of the 61 species known to occur in Sierra Leone (49.2%). The 42 bat species recorded in a wider area spanning 2,220 km2, which corresponds to 3% of the country surface (71,740 km2), represent more than two thirds of the national species pool (68.9%). These figures suggest that the assessment of bats on a national scale is also not yet complete.
The diverse habitat mosaic of Bumbuna II was reflected in the composition of bat species, with similar proportions of savannah (36.7%) and forest (43.3%) bat species, the latter being slightly dominant. However, the presence of bat species differed between seasons. For instance, the proportion of fruit bats in overall captures varied greatly, with 18.5% in three species at the onset of the wet season (2014) and 84.2% in eight species in the dry season (2016). This is probably driven by seasonal and spatial distribution of resources, while interannual variability might also play a role.
Bumbuna II harboured a high proportion of obligatorily and partially cave-roosting bats (Table
The species accumulation curve for terrestrial small mammals rises continuously and does not start to level off (Fig.
The high proportion of savannah species (46.2%) contrasts the results for bats, and might result from over-proportional sampling of savannah habitat and low mobility of terrestrial small mammals. However, three species associated with savannah (C. gambianus, G. kempi, U. ruddi) were only found in Bumbuna II, and three forest-dependent species (C. jouvenetae, C. emini, H. planifrons) only in Bumbuna I. The forest species Praomys rostratus was the most common terrestrial small mammal species in Bumbuna II, as in Bumbuna I (
Lophuromys sikapusi was rare in Bumbuna II (beginning of wet season), but common in Bumbuna I (beginning of dry season). This might support that L. sikapusi does not reproduce during the dry season as has been suggested by
The combined findings from Bumbuna II and I underline that high habitat heterogeneity in the transition zone between forests and savannahs in West Africa supports both forest-dependent and savannah species and fosters species richness, as has been previously reported for bats (
We would like to thank Phil Atkinson, British Trust of Ornithology (BTO), and Walter Bruton and Eimear Gormally, Environmental Resources Management (ERM), for initiating and preparing this project. This study was funded by Joule Africa. We are grateful to Kate M.B. Garnett of the Ministry of Agriculture, Forestry and Food Security (MAFF) in Sierra Leone for issuing export permits for voucher specimens. We greatly appreciate the essential, dedicated assistance of our counterparts and lead assistants, Jerry C. Garteh, Society for the Conservation of Nature of Liberia (SCNL), and Minkailu Bantama. Many thanks to Suzy Coey (ERM) for her support in survey logistics and feasibility, and to Allan Archer and Emily Coleman (BTO) for project organisation and administration. We are thankful to Jakob Fahr for sharing unpublished distribution data from the AfriBats database. Hannah Petersen (ZFMK) kindly performed the wet lab work. Rainer Hutterer (ZFMK) was of great help in identifying terrestrial small mammals, especially shrews, and Juliane Schaer provided input to the interpretation of molecular analyses of bats. Suzanne Livingstone and Emma Tatum-Hume, The Biodiversity Consultancy (TBC), have provided input on the project status. Thanks to Matt Cooper and Matt Rake (Joule Africa), and Mark Sanders-Crook (ERM) for managing the camps and logistic support. Annika Hillers, Royal Society for the Protection of Birds (RSPB), kindly lent us the harp trap of Gola Rainforest National Park for this study. Our work would not have been possible without the guidance and field support of our local assistants.
Field work for this study was funded by Joule Africa, via BTO and ERM. However, this had no role in study design, data collection and analyses, and manuscript writing and the authors declare no conflict of interest.
Microhabitat: proportion of ground cover types for terrestrial small mammals captured in Bumbuna II in 2014, based on percentage estimates at each trap (ca. 1x1m).