Biodiversity Data Journal : Taxonomic Paper
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Taxonomic Paper
Distoseptispora bambusae sp. nov. (Distoseptisporaceae) on bamboo from China and Thailand
expand article infoYaru Sun‡,§, Ishani D. Goonasekara§, Kasun M. Thambugala|, Ruvishika S. Jayawardena§, Yong Wang, Kevin D. Hyde§,
‡ Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang, China
§ Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand
| Genetics and Molecular Biology Unit, Faculty of Applied Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
¶ Institute of Plant Health, Zhongkai University of Agriculture and Engineering, Haizhu District, Guangzhou, China
Open Access

Abstract

Background

Bamboo is a widespread plant with medicinal value. During our taxonomic study on medicinal plants, three collections of Distoseptispora were made from China and Thailand. Phylogenetic analyses of combined LSU, ITS and RPB2 sequence data showed that two collections represented a new species, phylogenetically distinct from other described species in Distoseptispora.

New information

This new species has macronematous, mononematous conidiophores, polyblastic or monoblastic conidiogenous cells and acrogenous, solitary, straight, obclavate, multi-septate, thick-walled conidia. Distoseptispora bambusae sp. nov. is introduced with illustrations and a comprehensive description. The third collection on dead wood from Thailand was identified as D. tectona with newly-generated molecular data for this taxon.

Keywords

One new taxon, Distoseptisporales, hyphomycete, multi-gene phylogeny, taxonomy

Introduction

Distoseptispora was introduced by Su et al. (2016) with Distoseptispora fluminicola McKenzie, H.Y. Su, Z.L. Luo & K.D. Hyde as the type species. Distoseptispora has macronematous, septate, unbranched, straight or flexuous, smooth, olivaceous to brown conidiophores; mono- or polyblastic, holoblastic, determinate, terminal, cylindrical conidiogenous cells and acrogenous, solitary, olivaceous to brown, euseptate or distoseptate conidia (Su et al. 2016, Luo et al. 2018, Yang et al. 2018, Hyde et al. 2019, Luo et al. 2019). The monotypic family Distoseptisporaceae was established to accommodate Distoseptispora in Sordariomycetes (Su et al. 2016, Hyde et al. 2020). The freshwater genus Aquapteridospora J. Yang, K.D. Hyde & Maharachch was introduced by Yang et al. (2015) and was treated as Diaporthomycetidae genera incertae sedis, based on LSU sequence data. In a comprehensive study of freshwater Sordariomycetes, Luo et al. (2019) established Distoseptisporales and placed Distoseptisporaceae and Aquapteridospora within this order. Aquapteridospora differs from Distoseptispora in having polyblastic conidiogenous cells, bearing tiny, circular scars and protuberant, fusiform conidia. This treatment was followed by Hyde et al. (2020). However, Wijayawardene et al. (2020) only accepted Distoseptisporaceae in Distoseptisporales, while Aquapteridospora was placed in Diaporthomycetidae genera incertae sedis.

Currently, 25 species are accepted in Distoseptospora, of which 16 are from freshwater habitats and nine from terrestrial (Luo et al. 2018, Tibpromma et al. 2018, Crous et al. 2019, Hyde et al. 2019, Luo et al. 2019, Phookamsak et al. 2019). Distoseptispora caricis is the only reported endophytic species, while the others are saprobes.

During ongoing surveys of microfungi on medicinal plants, two Distoseptispora species were collected in China and Thailand. We introduce Distoseptispora bambusae as a novel taxon with illustrations and molecular phylogenetic data. We also provide newly-generated molecular data of the second species, D. tectona Doilom & K.D. Hyde, which was also reported from Thailand (Hyde et al. 2016).

Materials and methods

Collections and examination of specimens

Specimens of bamboo culms were collected from Guiyang, Guizhou Province, China (August 2019) and Doi Mae Salong, Chiang Rai, Thailand (July 2015). Another specimen of dead wood was collected from the Botanical Garden, Mae Fah Luang University, Chiang Rai, Thailand (November 2019). The samples were processed and examined following the method described by Dai et al. (2017). Samples were brought to the laboratory in an envelope after recording the collection details including hosts, places and dates. Morphological observations were made using a stereomicroscope (SteREO Discovery. V12, Carl Zeiss Microscopy GmBH, Germany). Fruiting bodies were transferred with a needle and placed in a drop of distilled water on a glass slide, then covered with the cover slip for microscopic studies and photomicrography. The morphological figures were captured using a Nikon ECLIPSE Ni compound microscope (Nikon, Japan) fitted with a NikonDS-Ri2 digital camera (Nikon, Japan). Measurements were made using the Tarosoft (R) Image Frame Work software. Photo-plates were made with Adobe Photoshop CS6 software (Adobe Systems, USA).

Single-spore isolations were done following the method described in (Chomnunti et al. 2014). Germinated spores were transferred to potato dextrose agar (PDA: 39 g/l sterile distilled water, Difco potato dextrose) plates and incubated at room temperature for 4 weeks. Herbarium materials were deposited in the Fungarium of Mae Fah Luang University (MFLU), Chiang Rai, Thailand. Pure cultures were deposited in the Mae Fah Luang University Culture Collection (MFLUCC) and International Collection of Microorganisms from Plants (ICMP). Facesoffungi (FoF) and Index Fungorum numbers were acquired as described in Jayasiri et al. (2015) and Index Fungorum (http://www.indexfungorum.org).

DNA extraction, PCR amplification and sequencing

Fresh fungal mycelia were scraped with sterilised scalpels. Genomic DNA was extracted using Genomic DNA Extraction Kit (GD2416) following the manufacture’s protocol. PCR amplifications were performed in a 20 μl reaction volume, with 10 μl of 10 × PCR Master Mix, 1 μl of each primer, 1 μl template DNA and 7 μl ddH2O. Primers used and PCR thermal cycle programmers are listed in Table 1.

Primers and PCR protocols.

Locus

Primer

PCR protocol

Reference

Internal Transcribed Spacer (ITS)

ITS5

ITS4

1. 94℃ – 3 min

2. 94℃ – 30 s

3. 52℃ – 30 s

4. 72℃ – 1 min

5. Repeat 2–4 for 35 cycles

6. 72℃ – 8 min

7. 4℃ on hold

White et al. (1990)

Large Subunit rRNA (LSU, 28S)

LR0R

LR5

Same protocol as ITS region

White et al. (1990), Rehner and Samuels (1995)

RNA polymerase II Subunit 2 (RPB2)

RPB2-5f

RPB2-7cR

1. 94℃ – 3 min

2. 94℃ – 20 sec

3. 55℃ – 30 sec

4. 72℃ – 1 min

5. Repeat 2–4 for 40 cycles

6. 72℃ – 10 min

7. 4℃ on hold

Liu et al. (1999)

Phylogenetic analyses

Sequences (Table 2) generated during this study were complemented with sequences from previous studies (Hyde et al. 2016, Su et al. 2016, Luo et al. 2018, Crous et al. 2019, Hyde et al. 2019, Luo et al. 2019), which were downloaded from NCBI GenBank (https://www.ncbi.nlm.nih.gov/genbank/). Alignments for each locus were done in MAFFT v7.212 (Katoh and Standley 2013) and checked visually using AliView (Larsson 2014). The alignments were trimmed using trimAl v 1.2 with gappyout (Capella-Gutiérrez et al. 2009). Three single gene alignments were combined using Sequence Matrix (Vaidya et al. 2011). The final alignment was deposited in TreeBASE (submission ID: http://purl.org/phylo/treebase/phylows/study/TB2:S26081).

GenBank accession numbers of isolates included in this study.

The newly-obtained strains are are indicated with after collection number. Ex-type strains are in bold.

Abbreviation: CBS: CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands; CGMCC: China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China; DLUCC: Dali University Culture Collection, Yunnan, China; HKUCC: The University of Hong Kong Culture Collection, Hong Kong, China; HKAS: Kunming Institute of Botany Academia Sinica, Yunnan, China, ICMP: International Collection of Microorganisms from Plants, Auckland, New Zealand; MFLU: the herbarium of Mae Fah Luang University, Chiang Rai, Thailand; MFLUCC: Mae Fah Luang University Culture Collection, Chiang Rai, Thailand.

Additional sequence of D. bambusae MFLUCC 20–0091: SSU: MT232716, TEF: MT232880

Species

Strain

ITS

LSU

RPB2

Aquapteridospora lignicola

MFLUCC 15–0377

KU221018

Aquapteridospora fusiformis

MFLU 18–1601

MK828652

MK849798

D. aquatica

MFLUCC 15–0374

NR154040

KU376268

D. aquatica

S-965

MK828647

MK849792

MN124537

D. aquatica

MFLUCC 18–0646

MK828648

MK849793

D. aquatica

MFLUCC 16–0904

MK828649

MK849794

D. aquatica

MFLUCC 16–1254

MK849795

D. aquatica

MFLUCC 16–1357

MK828650

MK849796

D. bambusae

MFLUCC 20–0091

MT232713

MT232718

MT232881

D. bambusae

MFLUCC 14–0583

MT232712

MT232717

MT232882

D. cangshanensis

MFLUCC 16–0970

MG979754

MG979761

D. caricis

CBS 146041

MN562124

MN567632

MN556805

D. dehongensis

KUMCC 18–0090

MK085061

MK079662

D. fluminicola

MFLUCC 15–0417

NR154041

KU376270

D. fluminicola

DLUCC 0391

MG979755

MG979762

D. fluminicola.

DLUCC 0999

MG979756

MG979763

D. guttulata

MFLUCC 16–0183

MF077543

MF077554

D. guttulata

DLUCC B43

MN163011

MN163016

D. leonensisi

HKUCC 10822

DQ408566

DQ435089

D. lignicola

MFLUCC 18–0198

MK828651

MK849797

D. martini

CGMCC 3.18651

KU999975

KX033566

D. multiseptata

MFLUCC 16–1044

MF077544

MF077555

MF135644

D. multiseptata

MFLUCC 15–0609

KX710145

KX710140

D. multiseptata

MFLUCC 18–0215

MN163013

MN174864

D neorostrata

MFLUCC 18–0376

MN163008

MN163017

D. obclavata

MFLUCC 18–0329

MN163012

MN163010

D. obpyriformis

MFLUCC 17–1694

MG979764

MG988415

D. obpyriformis

DLUCC 0867

MG979757

MG979765

MG988416

D. palmarum

MFLUCC 18–1446

MK085062

MK079663

MK087670

D. phangngaensis

MFLUCC 16–0857

MF077545

MF077556

D. rostrata

MFLUCC 16–0969

MG979758

MG979766

MG988417

D. rostrata

DLUCC 0885

MG979759

MG979767

D. submersa

MFLUCC 16–0946

MG979760

MG979768

MG988418

D. suoluoensis

MFLUCC 17–1305

MF077547

MF077558

D. suoluoensis

MFLUCC 17–0224

MF077546

MF077557

Distoseptispora. sp

HLXM–15–1

KU376269

D. rayongensis

MFLUCC 18–0415

MH457172

MH457137

MH463255

D. rayongensis

MFLUCC 18–0416

MH457173

MH457138

MH463256

D. tectonae

MFLUCC 12–0291

KX751711

KX751713

KX751708

D. tectonae

MFLUCC 20–0090

MT232714

MT232719

D. tectonigena

MFLUCC 12–0292

KX751712

KX751714

KX751709

D. thailandica

MFLUCC 16–0270

MH275060

MH260292

D. thysanolaenae

HKAS 102247

NR164041

MK064091

D. xishuangbannaensis

KUMCC 17–0290

MH275061

MH260293

MH412754

The Maximum Likelihood (ML) analysis was performed using IQ-tree (Nguyen et al. 2015, Chernomor et al. 2016). Nucleotide substitution models were selected under the Akaike Information Criterion (AIC) by jModelTest2 (Darriba et al. 2012) on XSEDE in the CIPRES web portal (Miller et al. 2010). For ITS dataset, the GTR+I+G model was selected (-lnL=3364.5406), for LSU, the TIM2+I+G model (-lnL = 959.3999), and for RPB2, the GTR+I+G (-lnL= 5111.0788). ML was inferred under partitioned models. Non-parametric bootstrap analysis was implemented with 1000 replicates.

Maximum Parsimony (MP) analysis was carried out with the heuristic search in PAUP v. 4.0b10 (Swofford 2002). All characters were unordered and of equal weight, and gaps were treated as missing data. Maxtrees were unlimited, branches of zero length were collapsed and all multiple, equally-parsimonious trees were saved. Clade stability was assessed using a bootstrap (BT) analysis with 1,000 replicates, each with 10 replicates of random stepwise addition of taxa (Hillis and Bull 1993).

Bayesian Inference (BI) analysis was performed by the Markov Chain Monte Carlo sampling (MCMC) coalescent approach implemented in BEAST v1.8.4 (Drummond et al. 2012), with an uncorrelated lognormal relaxed clock. The Birth-Death Incomplete Sampling speciation model (Stadler 2009) was selected as tree prior. The nucleotide substitution models were the same as above. Markov chains were run for 1,000,000 generations and trees were sampled every 1000th generation. The XML file generated by BEAUti (Drummond et al. 2012) was run using BEAST on XSEDE in the CIPRES web portal (Miller et al. 2010). Tracer v1.6 (Rambaut et al. 2014) was used to check the resulting log file. The first 20% of trees, representing the burn-in phase of the analyses, were discarded and a Maximum Clade Credibility tree was inferred using TreeAnnotator 1.8.4.

Trees were visualised with FigTree v1.4.4 (Rambaut 2009) and the layout was edited using Adobe Illustrator CS6 software (Adobe Systems, USA).

Taxon treatments

Distoseptispora bambusae Y.R. Sun, I.D. Goonasekara, Yong Wang bis & K.D. Hyde, sp. nov.

Materials    Download as CSV 
Holotype:
  1. scientificName:
    Distoseptispora bambusae
    ; class:
    Sordariomycetes
    ; order:
    Distoseptisporales
    ; family:
    Distoseptisporaceae
    ; country:
    China
    ; stateProvince:
    Guizhou
    ; locality:
    Guiyang Medicinal Plants Garden
    ; verbatimElevation:
    1100 m
    ; catalogNumber:
    MFLU 20–0261
    ; recordedBy:
    Sun Ya-Ru
    ; identifiedBy:
    Yaru Sun
    ; dateIdentified:
    2019
Paratype:
  1. scientificName:
    Distoseptispora bambusae
    ; class:
    Sordariomycetes
    ; order:
    Distoseptisporales
    ; family:
    Distoseptisporaceae
    ; country:
    Thailand
    ; stateProvince:
    Chiangrai
    ; locality:
    Doi Mae Salong
    ; verbatimElevation:
    390 m
    ; catalogNumber:
    MFLU 17–1653
    ; recordedBy:
    Thambugala Kasun M.
    ; identifiedBy:
    Yaru Sun
    ; dateIdentified:
    2019

Description

Saprobic on culms of bamboo. Sexual morph: Undetermined. Asexual morph: Hyphomycetous (Figs 1, 2). Colonies effuse, brown to dark-brown, hairy. Mycelium mostly immersed, composed of pale to dark brown, septate, branched, smooth, hyaline to subhyaline hyphae. Conidiophores macronematous, mononematous, septate, single or in groups of 2 or 3, erect, cylindrical, straight or slightly flexuous, olivaceous or brown, robust at the base 40–96 × 4–5.5 μm (x̅ = 69 × 5 μm, n = 10). Conidiogenous cells blastic, integrated, terminal, cylindrical, olivaceous or brown 9–19 × 4–5 μm (x̅ = 15 × 4.5 μm, n = 15). Conidia acrogenous, solitary, straight, obclavate, septate, thick-walled, rounded at the apex, truncate at the base, tapering towards apex, olivaceous or brown, 45–74 μm long (x̅ = 60.5 μm, n = 20), 5.5–9.5 μm at the widest (x̅ = 7.5 μm, n = 20).

Figure 1.  

Distoseptispora bambusae (MFLU 20–0261, holotype, collected from China) a, b. Colonies on natural substrate; c, d. Conidiophore with Conidia; e. Conidiophore; f. Conidiogenous cell; gj. Conidia; k. Germinating conidium; l, m. Colony on PDA. Scale bars: c–e, k = 20 μm, f–j = 10 μm.

Figure 2.  

Distoseptispora bambusae (MFLU 17–1653, paratype, collected from Thailand). a, b. Colonies on natural substrate; cg. Conidia attached to conidiophores; hk. Conidia; l. Germinating conidium. Scale Bars: c = 50 μm, d–l = 25 μm.

Culture characteristics: Conidia germinated on PDA within 12 hours and germ tubes were produced from both ends. Colony reached 30 mm in 4 weeks at 26℃ on PDA media, circular, flat, surface rough, grey from above, brown from below, edge entire.

Notes

The morphological characteristics of Distoseptispora bambusae match well with the generic concept of Distoseptispora (Su et al. 2016). Multi-gene analyses showed that D. bambusae is a phylogenetically-distinct species, most closely related to D. suoluoensis, a species isolated from submerged wood in a freshwater habitat (Yang et al. 2018). Distoseptispora bambusae has shorter conidiophores (40–96 vs. 80–250 μm) and shorter conidia (45–74 vs. (65–) 80–125(–145) μm) than those of D. suoluoensis (Yang et al. 2018). Our two specimens of D. bambusae were similar in morphology, but polyblastic conidiogenous cells were observed from the Chinese specimen, while the Thai specimen has only monoblastic conidiogenous cells. These may be due to geographical differences and the different observation period. Although the two strains clustered together with short branches in the phylogenetic tree, comparisons of ITS sequences showed that there are 3 bp (base pair) differences without gaps between two strains and we identified them as the same species following the guidelines for species delineation proposed by Jeewon and Hyde (2016).

Etymology

Bambusae, referring to the host.

Distoseptispora tectonae Doilom & K.D. Hyde Fungal Diversity 81: 222 (2016)

Material    Download as CSV 
  1. scientificName:
    Distoseptispora tectonae
    ; class:
    Sordariomycetes
    ; order:
    Distoseptisporales
    ; family:
    Distoseptisporaceae
    ; country:
    Thailand
    ; stateProvince:
    Chiangrai
    ; locality:
    Mae Fah Luang University, Botanical Garden
    ; verbatimElevation:
    390 m
    ; catalogNumber:
    MFLU 20–0262

Description

Saprobic on stems of dead wood. Sexual morph: Unknown. Asexual morph: Hyphomycetous (Fig. 3). Colonies effuse, brown to dark brown, hairy. Mycelium mostly immersed, composed of brown, septate, branched hyphae. Conidiophores macronematous, mononematous, septate, single or in groups of two, straight or slightly flexuous, cylindrical, dark brown, 34–95 × 5–8 μm (x̅ = 61.5 × 6 μm, n = 15). Conidiogenous cells integrated, terminal, monoblastic, cylindrical, brown. Conidia acrogenous, solitary, straight or slightly flexuous, rostrate, 11–23-distoseptate, differently constricted at the septa, thick-walled, truncate at the base, tapering towards apex, brown at the base, pale brown at the apex, 89–176 μm long (x̅ = 121 μm, n = 25), 12–19 μm at the widest (x̅ = 15 μm, n = 25).

Figure 3.  

Distoseptispora tectonae (MFLU 20–0262). a. Colonies on natural substrate; b. Conidiogenous cell; ce. Conidiophores and conidia; fi. Conidia; j. Germinating conidium. Scale bars: b = 10 μm, a, c–j = 50 μm.

Culture characteristics: Conidia germinated on PDA within 12 hours and germ tubes were produced from both ends. On PDA, colony circular, reaching 40 mm diam after 4 weeks at 26℃, brown from above, dark brown from below, surface flat and slightly rough, edge entire.

Notes

Distoseptispora tectonae was introduced by Hyde et al. (2016), from a terrestrial habitat in Thailand. Distoseptispora tectonae has macronematous, cylindrical, septate conidiophores, monoblastic, integrated, terminal, cylindrical conidiogenous cells and obclavate, straight or slightly curved, septate, smooth conidia. Our collection was also from Thailand. The morphological characters of our collection are the same as in the holotype, except that our isolate has longer and wider conidiophores (34–95 × 5–8 μm vs. up to 40 × 4–6 μm) and less septa (11–23 vs. 20–28), compared to those of D. tectonae MFLUCC 12–0291. In this study, we also provide new sequences for D. tectonae.

Analysis

Partial nucleotide sequences of the LSU, ITS and RPB2 were used to determine the phylogenetic position of the taxa isolated. Sequences of 47 strains retrieved from GenBank, representing species of Distoseptispora and two outgroups A. fusiformis (MFLU 18–1601) and A. lignicola (MFLUCC 15–0377), were analysed. Single gene analyses were done to compare the topologies and clade stabilities, respectively. Nucleotide substitution models were selected by jModelTest2 on XEDE (Drummond et al. 2012). For the ITS and RPB2 dataset, the GTR+I+G model was selected, for LSU, the TIM2+I+G. The manually-adjusted LSU, ITS and RPB2 alignment comprised a total of 2,246 characters (768 for LSU; 436 for ITS; 1,042 for RPB2), including coded alignment gaps. Amongst them, 1,471 characters were constant, 195 variable characters were parsimony-uninformative and number of parsimony-informative characters was 580. One thousand equally most parsimonious trees (Tree length = 1799, CI = 0.640, RI = 0.733, RC = 0.469, HI = 0.360) were yielded from the heuristic search. MP, ML and Bayesian analyses of the combined dataset inferred similar topologies, respectively. The "most likelihood" tree is presented (Fig. 4).

Figure 4.  

Maximum Likelihood (RAxML) tree, based on analysis of a combined dataset of LSU, ITS and RPB2 sequence data. Bootstrap support values for ML and MP greater than 75% and Bayesian posterior probabilities greater than 0.95 are given near nodes, respectively. The tree is rooted with Aquapteridospora fusiformis (MFLU 18–1601) and A. lignicola (MFLUCC 15–0377). The ex-type strains are indicated in bold and the new isolates are in red.

In the phylogenetic analyses, generated by ML, MP and BI analysis, the two Distoseptispora bambusae isolates clustered with strong support (80%, 92%, 0.97). They formed a sister clade with D. suoluoensis with high support (95%, 94%, 0.95). Our isolate D. tectonae (MFLUCC 20–0090) grouped with D. tectonae (MFLUCC 12–0291) with strong ML, MP and BI support (96%, 80%, 0.97), indicating they are the same species.

Discussion

In this study, two collections from China and Thailand, representing a new Distoseptispora species, is introduced, based on morphology and phylogenetic analysis. The two samples were both found on bamboo from terrestrial habitats. It is the fourth species found from medicinal plants. The other three are D. palmarum, D. thailandica and D. xishuangbannaensis (Tibpromma et al. 2018, Hyde et al. 2019).

Distoseptispora species does not seem to have specific habitat preferences. Most of them are reported from submerged wood in freshwater habitats, while some species have been introduced from terrestrial habitats (Luo et al. 2018, Tibpromma et al. 2018, Hyde et al. 2019, Luo et al. 2019, Phookamsak et al. 2019). So far, Distoseptispora were only found in China and Thailand. They may exist in other countries, waiting to be discovered on the basis of their diverse habitats.

The asexual morph of Distoseptispora is similar to Sporidesmium in producing holoblastic, euseptate or distoseptate conidia and blastic, terminal conidiogenous cells (Shenoy et al. 2006, Luo et al. 2018, Yang et al. 2018). Sexual morphs of Distoseptispora have not been reported.

Acrodictys martini was transferred to Distoseptispora as D. martini by Xia et al. (2017), based on their phylogenetic analysis. However, this species morphologically resembles Acrodictys rather than Distoseptispora. Therefore, the molecular data of Distoseptispora martini may need further verification (Luo et al. 2018).

It is interesting to note that, in most species of Distoseptispora, the conidia are longer than their conidiophores, while in some, they are shorter than their conidiophores. However, this characteristic does not reflect their phylogenetic position. For example, D. obpyriformis Z.L. Luo & H.Y. Su, a species that has long conidia and short conidiophores and D. rostrata Z.L. Luo, K.D. Hyde & H.Y. Su that has longer conidiophores, but shorter conidia, form a sister clade in the phylogenetic tree.

Acknowledgements

This research is supported by the following projects: National Natural Science Foundation of China (No. 31972222, 31560489), Program of Introducing Talents of Discipline to Universities of China (111 Program, D20023), Science and Technology basic work of MOST [2014FY120100], Talent project of Guizhou Science and Technology Cooperation Platform ([2017]5788-5 and [2019]5641) and Guizhou Science, Technology Department International Cooperation Basic project ([2018]5806), Construction Program of Biology First-class Discipline in Guizhou (GNYL[2017]009), Guizhou University cultivation project [2017]5788-33, Science and technology major project of Guizhou Province ([2019]3005). We would like to thank Dr. Eric H. C. McKenzie for suggestions on the morphology and Dr. Shaun Pennycook for checking the nomenclature. Kevin D. Hyde would thank the Thailand Research grants entitled “Impact of climate change on fungal diversity and biogeography in the Greater Mekong Subregion” (grant no: RDG6130001).

References