A new genus of Bambusicolaceae (Pleosporales) on Corylus avellana (Fagales) from Italy

Subodini Wijesinghe; Yong Wang; Erio Camporesi; Dhanushka Wanasinghe; Saranyaphat Boonmee; Kevin Hyde

doi:10.3897/BDJ.8.e55957

Biodiversity Data Journal : Taxonomic Paper

Taxonomic Paper

A new genus of Bambusicolaceae (Pleosporales) on Corylus avellana (Fagales) from Italy

Subodini Nuwanthika Wijesinghe^‡,§,|, Yong Wang^‡, Erio Camporesi^¶, Dhanushka Nadeeshan Wanasinghe^#, Saranyaphat Boonmee^§,|, Kevin David Hyde^§,#,¤

‡ Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, Guizhou Province, 550025, China

§ Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand

| School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand

¶ A.M.B. Gruppo Micologico Forlivese “Antonio Cicognani”, Via Roma 18, Forlì, Italy

# CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, Yunnan, China

¤ Innovative Institute of Plant Health, Zhongkai University of Agriculture and Engineering, Haizhu District, Guangzhou 510225, China

Corresponding author: Yong Wang (yongwangbis@aliyun.com)

Academic editor: Danny Haelewaters

Received: 29 Jun 2020 | Accepted: 16 Jul 2020 | Published: 19 Aug 2020

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation: Wijesinghe SN, Wang Y, Camporesi E, Wanasinghe DN, Boonmee S, Hyde KD (2020) A new genus of Bambusicolaceae (Pleosporales) on Corylus avellana (Fagales) from Italy. Biodiversity Data Journal 8: e55957. https://doi.org/10.3897/BDJ.8.e55957

Abstract

Background

In this study, we introduce Corylicola gen. nov. in the family of Bambusicolaceae (Pleosporales), to accommodate Corylicola italica sp. nov. The new species was isolated from dead branches of Corylus avellana (common hazel) in Italy. The discovery of this new genus with both sexual and asexual characters will contribute to expand the knowledge and taxonomic framework of Bambusicolaceae.

New information

Corylicola gen. nov. has similar morphological characters compared to other genera of Bambusicolaceae. These are solitary, scattered, globose to subglobose and ostiolate ascomata; anastomosing and branching pseudoparaphyses; cylindrical asci with a well-developed ocular chamber and short furcate pedicel; and single-septate ascospores. The coelomycetous asexual morph of Corylicola has holoblastic, phialidic conidiogenous cells and light brown conidia analogous to other members in the family. Corylicola differs from the other genera of Bambusicolaceae in having yellowish-brown ascospore masses at the top of the ascomatal neck. Detailed morphological illustrations with comprehensive descriptions for the new taxa are provided, as well as a key to the genera of Bambusicolaceae. Maximum Likelihood analysis and Bayesian Inference of a combined SSU, LSU, ITS, RPB2 and TEF1 sequence dataset confirms the placement of this genus as a distinct lineage in Bambusicolaceae.

Keywords

Bambusicolous fungi, Dothideomycetes, phylogeny, taxonomy

Introduction

Bambusicolaceae (Pleosporales) was introduced in Dothideomycetes by Hyde et al. (2013) to accommodate Bambusicola (Dai et al. 2012, Liu et al. 2015, Jayasiri et al. 2019, Yang et al. 2019). Initially, this family included only three genera: Bambusicola, Neobambusicola (Crous et al. 2014) and Palmiascoma (Liu et al. (2015). Later, Longipedicellata (Zhang et al. 2016) and Leucaenicola (Jayasiri et al. 2019) were also introduced. Tanaka et al. (2015) revised the pleosporalean sub-order Massarineae and transferred Neobambusicola to Sulcatisporaceae. Phukhamsakda et al. (2016) excluded Longipedicellata from Bambusicolaceae and introduced a new family to accommodate this genus, Longipedicellataceae. As a result, currently, Bambusicolaceae includes three genera. These are Bambusicola, Leucaenicola and Palmiascoma (Wijayawardene et al. 2020).

Species of Bambusicolaceae are characterised by solitary, scattered, immersed, semi-immersed to erumpent and conical or globose to subglobose ascomata; anastomosing, branching interascal filaments; cylindrical to clavate asci with a short furcate or rounded to obtuse pedicel; and slightly broad-fusiform or clavate to ellipsoidal, hyaline or yellowish to brown, single-septate ascospores with gelatinous sheath (Dai et al. 2012, Hyde et al. 2013, Liu et al. 2015, Dai et al. 2017). Coelomycetous asexual characteristics of Bambusicolaceae are pycnothyrial or pycnidial conidiomata, holoblastic or enteroblastic and phialidic or annelidic conidiogenous cells with hyaline or pale to dark brown, cylindrical or oblong to ellipsoidal aseptate to 1–3-septate conidia (Dai et al. 2012, Hyde et al. 2013, Liu et al. 2015, Dai et al. 2017, Jayasiri et al. 2019). Members of the family Bambusicolaceae share morphological characters with families Didymosphaeriaceae, Massarinaceae and Tetraplosphaeriaceae in the order Pleosporales, such as cylindrical to clavate asci and fusiform to ellipsoidal, hyaline to brown, single-septate ascospores (Tanaka et al. 2009, Zhang et al. 2009, Dai et al. 2012, Hyde et al. 2013, Dai et al. 2015). However, its asexual characteristics are different from these families (Dai et al. 2015).

Bambusicola was introduced by Dai et al. (2012) and placed in Trematosphaeriaceae, based on the phylogenetic analysis of a large subunit (LSU) ribosomal DNA dataset by maximum parsimony. Bambusicola, with type species B. massarinia (Wijayawardene et al. 2017), is characterised by small, cone-shaped ascomata; slightly broad and fusiform, hyaline ascospores; and coelomycete asexual morphs with light brown conidia (Dai et al. 2012, Yang et al. 2019). A multi-locus phylogenetic analysis conducted by Hyde et al. (2013) resulted in the placement of Bambusicola in Bambusicolaceae. Twelve species are known in this genus according to Index Fungorum (2020). Palmiascoma was introduced by Liu et al. (2015) from palms, based on morpho-molecular analyses. This monotypic genus is characterised by clavate asci with rounded to obtuse pedicels and clavate to ellipsoidal, yellowish-brown to dark brown, echinulate, single-septate ascospores (Liu et al. 2015). Leucaenicola is an asexual genus introduced by Jayasiri et al. (2019) from decaying pods of Leucaena species. It is characterised by conidial morphology, size and colour that are similar to those of the micro-conidia of B. thailandica, but are phylogenetically distinct (Jayasiri et al. 2019). Three species are currently described in Leucaenicola (Ariyawansa et al. 2020).

In this study, we introduce Corylicola gen. nov. to accommodate Corylicola italica sp. nov. isolated from Corylus avellana in Italy. We present morphological illustrations of both sexual and asexual morphs, comprehensive descriptions, phylogenetic analyses based on SSU, LSU, ITS, RPB2 and TEF1 sequence data and a key to genera in Bambusicolaceae to confirm the placement of the new genus in Bambusicolaceae.

Materials and methods

Specimens collection, examination and isolation

Dead branches with black raised spots on the surface were collected from Corylus avellana trees in Italy (February 2019). Samples were taken to the laboratory in a plastic Ziploc bag and stored inside paper envelopes. Samples were examined and processed following the procedure described by Wijesinghe et al. (2019). Photographs of enlarged host twigs and ascomata were taken using a Motic SMZ 168 compound stereomicroscope. Morphological characters were examined by hand sectioning of fruiting structures on the surface of twigs. The micro-morphological structures inside ascomata were photographed using a Nikon ECLIPSE 80i compound stereomicroscope with a Canon 600D digital camera. The following structures were observed and measured: diameter, height, colour and shape of ascomata and ostiole; peridium width, cell structure and colour; length and width of asci and ascospores (at the longest and widest point, respectively) and width of pseudoparaphyses. Tarosoft (R) Image Frame Work version 0.9.7. programme was used for the measurements of photomicrograph structures. Images used for figures were processed with Adobe Photoshop CS6 Extended version 13.0.1 software (Adobe Systems, San Jose, California).

Single-ascospore isolation was carried out following protocols described by Chomnunti et al. (2014). Single germinated ascospores were aseptically transferred on to potato dextrose agar (PDA) plates, which were incubated at 18°C for 15 to 20 days to obtain pure cultures. Colony characters were observed and measured weekly. After one month, cultures were used to extract DNA. Photographs of enlarged structures in culture were taken using a Motic SMZ 168 compound stereomicroscope. Micro-morphological characters were examined and photographed using a Nikon Eclipse Ni-U microscope with Nikon DS-RI2 microscope camera. All structures (conidiomata, hyphae, conidiomata wall, conidiogenous cells and conidia) were processed for photographs by using water-mounted glass slides. The holotype was deposited at MFLU (Mae Fah Luang University Herbarium, Chiang Rai, Thailand). The ex-type cultures were deposited at MFLUCC (Mae Fah Luang culture collection). Both Facesoffungi and Index Fungorum numbers were obtained (Jayasiri et al. 2015, Index Fungorum 2020).

DNA extraction, PCR amplification and sequencing

Genomic DNA was isolated from fruiting bodies and from scraped fresh fungal mycelium grown on PDA media for six weeks at 18°C, using the EZgene^TM Fungal gDNA extraction Kit GD2416 (Biomiga, Shanghai, China), following the manufacturer's instructions. DNA was stored at 4°C for use in regular work and at -20°C for long-term. Sequences were generated for five gene regions, small subunit (SSU), the internal transcribed spacer region (including ITS1, 5.8S, ITS2), large subunit (LSU), RNA polymerase II second largest subunit (RPB2) and translation elongation factor 1-α (TEF1). The following primers were used for PCR amplification: NS1 and NS4 for SSU, ITS5 and ITS4 for ITS, LR0R and LR5 for LSU, fRPB2-5F and fRPB2-7cR for RPB2 and EF1-983F and EF1-2218R for TEF1 (White et al. 1990, Vilgalys and Hester 1990, Hopple 1994, Rehner and Samuels 1994, Liu et al. 1999, Rehner 2001).

PCR was carried out in 20 μl reactions, containing 10.0 μl of Bench Top^TM Taq MasterMix PCR mixture (SinoGenoMax, Beijing, China), 1 μl of each forward and reverse primer (10 μM), 1 μl template genomic DNA and 7.0 μl deionised water. PCR protocols were as follows: For ITS and LSU: initial denaturation at 94°C for 2 mins; followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 55°C for 50 s, elongation at 72°C for 90 s; and final extension at 72°C for 10 min. For SSU: initial denaturation at 95°C for 3 mins; followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 55°C for 50 s, elongation at 72°C for 30 s; and final extension at 72°C for 10 min. For RPB2: initial denaturation at 94°C for 2 mins; followed by 35 cycles of denaturation at 95°C for 45 s, annealing at 57°C for 50 s, elongation at 72°C for 90 s; and final extension at 72°C for 10 min. Finally for TEF1: initial denaturation at 94°C for 2 mins; followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 58°C for 50 s, elongation at 72°C for 1 min; and final extension at 72°C for 10 min. The PCR products were verified by staining with ethidium bromide on 1% agarose electrophoresis gels. Sequencing of PCR amplicons was conducted with the same primers used for PCR. Sequencing of successfully amplified PCR products was outsourced to the SinoGenoMax Sanger sequencing laboratory (Beijing, China). Lasergene SeqMan Pro v.7 software (DNASTAR, Madison, Wisconsin) was used to obtain consensus sequences from generated sequence reads. Resulting sequences were deposited in NCBI GenBank (Table 1).

Table 1.

Download as

CSV

XLSX

Taxa used for molecular study and their GenBank numbers.

* Newly-generated sequences are indicated by ^▲ after the species name and type materials are in bold.

Abbreviation: CBS: CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands; KUMCC: Kunming Institute of Botany Culture Collection, Chinese Academy of Sciences, Kunming, China; MFLU: the Herbarium of Mae Fah Luang University, Chiang Rai, Thailand; MFLUCC: Mae Fah Luang University Culture Collection, Chiang Rai, Thailand; SICAU Herbarium of Sichuan Agricultural University, Chengdu, China.

Species	Strain /Voucher	SSU	LSU	ITS	TEF1	RPB2
*Bambusicola bambusae*	MFLUCC 11-0614	JX442039	JX442035	JX442031	KP761722	KP761718
*B. didymospora*	MFLUCC 10-0557	KU872110	KU863105	KU940116	KU940188	KU940163
B. didymospora	MFLUCC 15-0189	KU872111	KU863106	KU940117	KU940189	KU940164
*B. dimorpha*	MFLUCC 13-0282	KY038354	KY000661	KY026582	-	KY056663
*B. irregulispora*	MFLUCC 11-0437	JX442040	JX442036	JX442032	KP761723	KP761719
*B. loculata*	MFLU 15-0056	KP761735	KP761729	KP761732	KP761724	KP761715
*B. massarinia*	MFLUCC 11-0389	JX442041	JX442037	JX442033	KP761725	KP761716
B. massarinia	MFLUCC 11-0135	-	KU863111	KU940122	KU940192	KU940169
*B. pustulata*	MFLUCC 15-0190	KU872112	KU863107	KU940118	KU940190	KU940165
B. sichuanensis	SICAU 16-0004	MK253528	MK253532	MK253473	MK262828	MK262830
B. splendida	MFLUCC 11-0611	KU872114	KU863110	KU940121	-	KU940168
*B. splendida*	MFLUCC 11-0439	JX442042	JX442038	JX442034	KP761726	KP761717
*B. subthailandica*	SICAU 16-0005	MK253529	MK253533	MK253474	MK262829	MK262831
*B. thailandica*	MFLUCC 11-0147	KU872113	KU863108	KU940119	KU940191	KU940166
*B. triseptatispora*	MFLUCC 11-0166	-	KU863109	KU940120	-	KU940167
*Corylicola italica^▲*	MFLUCC 20-0111	MT633084	MT626713	MT633085	MT590777	MT635596
*Corylicola italica^▲*	MFLU 19-0500	MT554923	MT554926	MT554925	-	MT590776
*Latorua caligans*	CBS 576.65	-	MH870362	MH858723	-	-
*L. grootfonteinensis*	CBS 369.72	-	KR873267	-	-	-
*Leucaenicola aseptata*	MFLUCC 17-2423	MK347853	MK347963	MK347746	MK360059	MK434891
*L. phraena*	MFLUCC 18-0472	MK347892	MK348003	-	MK360060	MK434867
*Magnicamarosporium diospyricola*	MFLUCC 16-0419	KY554211	KY554212	KY554210	KY554209	KY554208
M. iriomotense	CBS 139696	AB797219	AB807509	AB809640	-	-
*Neoaquastroma guttulatum*	MFLUCC 14-0917	KX949741	KX949740	KX949739	KX949742	-
Neobambusicola brunnea	MFLU 18-1393	-	MH644791	MH644792	-	-
*N. strelitziae*	CBS 138869	-	KP004495	KP004467	MG976037	-
*Palmiascoma gregariascomum*	MFLUCC 11-0175	KP753958	KP744495	KP744452	-	KP998466
P. gregariascomum	KUMCC 19-0201	MT477186	MT477185	MT477183	-	MT495782
*Polyschema congolensis*	CBS 542.73	-	EF204502	MH860770	-	EF204486
*P. terricola*	CBS 301.65	EF204519	MH870213	MH858576	-	EF204487
*P. larviformis*	CBS 463.88	-	EF204503	-	-	-
*Sulcatispora acerina*	CBS 139703	LC014605	LC014610	LC014597	LC014615	-
*S. berchemiae*	CBS 139704	AB797244	AB807534	AB809635	AB808509	-

Phylogenetic analyses

Sequences with high similarity indices were determined by BLAST searching and relevant literature (Jayasiri et al. 2019, Yang et al. 2019). Contig sequences were analysed with other sequences downloaded from GenBank. The final alignment consists of the new species and sequences of the genera Bambusicola, Leucaenicola and Palmiascoma, along with representatives from other families (Latoruaceae, Parabambusicolaceae, Sulcatisporaceae) (Table 1). Single and multiple (SSU, LSU, ITS, RPB2 and TEF1) alignments of all sequences were performed with MAFFT v.7 (http://mafft.cbrc.jp/alignment/server/index.html, Katoh and Standley 2013, Katoh et al. 2017). Manual improvement, when necessary, was done using BioEdit v.7.0.5.2 software (Hall 1999). Terminal ends and ambiguous regions of the alignment were deleted manually.

Phylogenetic analyses of both individual and combined datasets were based on Maximum Likelihood (ML) and Bayesian Inference (BI). Both analyses were run on the CIPRES Science Gateway portal (Miller et al. 2012). For RAxML (Randomised Accelerated Maximum Likelihood) analysis, the sequence alignments were converted from FASTA into PHYLIP format using the ALTER (alignment transformation environment, http://www.sing-group.org/ALTER/) bioinformatics web tool (Glez-Pea et al. 2010). Maximum Likelihood trees were generated with RAxML-HPC2 on XSEDE (v.8.2.10) (Stamatakis 2014) using the GTR+GAMMA substitution model. The optimal ML tree search was conducted with 1,000 separate runs. Nonparametric bootstrap iterations were run with 1,000 replicates.

For BI, the sequence alignments were converted from FASTA into NEXUS format using ClustalX2 v.1.83 (Thompson et al. 1997). To estimate the best evolutionary models for each gene region, MrModeltest v.2.3 (Nylander 2004) was used under the Akaike Information Criterion (AIC) implemented in PAUP v.4.0b10 (Swofford 2003). The best-fit model was determined as GTR+I+G for each locus. Six simultaneous Markov chains were run for 1,000,000 generations and trees were sampled every 100^th generation. The distribution of log-likelihood scores was examined to determine the stationary phase for each search and to decide if extra runs were required to achieve convergence, using Tracer 1.5 (Rambaut and Drummond 2007). The first 10% of generated trees were discarded and remaining 90% of trees were used to calculate posterior probabilities (PP) of the 50% majority rule consensus tree. Phylograms were visualised with FigTree v.1.4.0 (Rambaut 2012) and modified in Microsoft PowerPoint (2010). The finalised alignment and tree were deposited in TreeBASE submission ID: 26617 (http://purl.org/phylo/treebase/phylows/study/TB2:S26617).

Data resources

Taxon treatments

Corylicola Wijesinghe, E. Camporesi, Yong Wang bis & K.D. Hyde, 2020, gen. nov.

IndexFungorum 557767

Type species

Corylicola italica Wijesinghe, Camporesi, Yong Wang bis & K.D. Hyde, 2020, sp. nov.

Description

Saprobic on dead branches of Corylus avellana L. Sexual morph Ascomata solitary, scattered, immersed to erumpent, globose to subglobose, coriaceous, uni-loculate with an ostiole. Ostiole central, papillate, lined with hyaline periphyses. Peridium fused with host tissues, unequally thick, outermost layer comprising blackish to dark brown cells of textura angularis, inner layer comprising hyaline cells of textura prismatica. Hamathecium comprising numerous, dense, filamentous, cellular pseudoparaphyses with distinct septa. Asci 8-spored, bitunicate, fissitunicate, cylindrical, pedicellate, with an ocular chamber. Ascospores uniseriate, fusiform to ellipsoidal, yellowish to pale brown, single-septate, echinulate, accumulating as yellowish-brown masses at the apices of ascomatal neck. Asexual morph: Coelomycetous. Conidiomata pycnidial, solitary to gregarious, scattered, semi-immersed to superficial, globose to subglobose, uni-loculate to multi-loculate, ostiolate. Ostiolate central and circular. Conidiomata wall composed of several layers of pale to dark brown, pseudoparenchymatous cells. Conidiophores reduced to conidiogenous cells. Conidiogenous cells holoblastic, phialidic, ampulliform, yellowish to pale brown, aseptate, smooth-walled. Conidia solitary, globose or oblong to ellipsoid, rounded or obtuse ends, yellowish to pale brown, aseptate, rarely guttulate, smooth-walled.

Facesoffungi Number:

FoF 08684

Etymology

Referring to the host genus, Corylus

Notes

Corylicola gen. nov. is a monotypic genus associated with Corylus avellana L., which is commercially important for hazelnut production (Nitride et al. 2017). The new genus is characterised by didymosporous, brown and echinulate ascospores, which are morphologically similar to Palmiascoma (Bambusicolaceae), Didymosphaeria, Munkovalsaria and Verruculina (Didymosphaeriaceae) (Zhang et al. 2012, Liu et al. 2015). Phylogenetically, however, Corylicola forms a distinct lineage within Bambusicolaceae (Fig. 1 B). Corylicola differs morphologically from Bambusicola and Palmiascoma by the accumulation of ascospores as yellowish-brown masses at the apices of ascomatal necks. Bambusicola, Leucaenicola and Palmiascoma have coelomycetous asexual morphs, which are similar to Corylicola (Dai et al. 2012, Liu et al. 2015, Jayasiri et al. 2019). Asexually, Corylicola is characterised by holoblastic, phialidic conidiogenous cells similar to Palmiascoma and one-celled, aseptate conidia similar to Palmiascoma and Leucaenicola, whereas Bambusicola has 1–3-septate conidia (Hyde et al. 2013, Liu et al. 2015, Dai et al. 2017). Based on morphological observations, a key to all four genera of the family is provided.

Figure 1.

Phylogram generated from Maximum Likelihood analysis, based on combined SSU, LSU, ITS, RPB2 and TEF1 sequence data for Bambusicolaceae. Maximum Likelihood bootstrap values (ML) ≥ 70% and posterior probabilities (PP) ≥ 0.95 are given above each node. The GenBank accession numbers are provided at the right side of the species names. Strains of the novel species are visualised in blue-bold and holotype materials are symbolized with ^H.

Corylicola italica Wijesinghe, Camporesi, Yong Wang bis & K.D. Hyde 2020, sp. nov.

IndexFungorum 557768

Materials Download as CSV

Holotype:

scientificName:
Corylicola italica Wijesinghe, Camporesi, Yong Wang bis & K.D. Hyde 2020
; phylum:
Ascomycota
; class:
Dothideomycetes
; order:
Pleosporales
; family:
Bambusicolaceae
; genus:
Corylicola
; stateProvince:
Province of Forlì-Cesena
; county:
Italy
; locality:
near Meldola
; year:
2019
; month:
March
; habitat:
Terrestrial
; fieldNotes:
on a dead hanging branch of Corylus avellana (Betulaceae)
; recordedBy:
Erio Camporesi
; identifiedBy:
S.N. Wijesinghe
; dateIdentified:
2019
; collectionID:
MFLU 19–0500
; collectionCode:
IT4211

Other material:

scientificName:
Corylicola italica
; type:
ex-type living culture
; occurrenceID:
MFLUCC 20–0111

Description

Saprobic on a dead, hanging branch of Corylus avellana L. Sexual morph: (Fig. 2), Ascomata 210–300 high, 200–260 µm diam. (x̄ = 261 × 227 µm), solitary, scattered, immersed, erumpent at maturity, raised as dark spots on the substrate, sessile, globose to subglobose, coriaceous, uni-loculate with an ostiole. Ostiole 50–65 µm long, 30–35 µm wide, central, papillate, lined with hyaline periphyses. Peridium composed of two layers, unequally thickened, 15–30 µm wide at the apex and 10–25 µm wide at the base, outermost layer comprising blackish to dark brown cells of textura angularis fused with host tissues, inner layer comprising hyaline cells of textura prismatica. Hamathecium comprising numerous pseudoparaphyses, which are 1–2 µm wide (x̄ = 2 µm, n =10), dense, filamentous, cellular, with distinct septa, not constricted at the septa, branching and anastomosing above the asci. Asci 55–65 × 6–8 µm (x̄ = 61 × 7 µm, n =20), 8-spored, bitunicate, fissitunicate, cylindrical, short distinct pedicel with furcate ends, apically rounded, well-developed ocular chamber. Ascospores 10–15 × 3–4 µm (x̄ = 12 × 3.5 µm, n =40), overlapping, uni-seriate, fusiform to ellipsoidal, straight, yellowish when young, becoming pale brown at maturity, single-septate, constricted at the septum, rounded at the apices, upper cell is wider than the lower cell (2–5 vs. 2–4 µm (x̄ = 4 vs. 3.25 µm, n =40), echinulate, guttulate. Asexual morph: (Fig. 3), Coelomycetous forming naturally on PDA media after 12 weeks. Conidiomata 175–200 high 150–170 µm diam. (x̄ = 183 × 161 µm) pycnidial, solitary to gregarious, scattered, semi-immersed to superficial, visible as black spore mass surrounded by cellular vegetative hyphae (1–2 µm width), globose to subglobose, glabrous, uni-loculate to multi-loculate, ostiolate. Ostiolate 45–50 µm long, 50–60 µm wide, central and circular. Conidiomata wall 7–20 µm wide, composed of several layers of pale to dark brown, pseudoparenchymatous cells, outermost layers comprising 3−5 layers of dark brown cells of textura prismatica to textura angularis, inner layers comprising 2−3 layers of pale brown to hyaline cells of textura angularis. Conidiophores reduced to conidiogenous cells, originated from the basal cavity of conidiomata. Conidiogenous cells 3–4.5 × 2–4 μm (x̄ = 3.6 × 3 μm, n = 30), holoblastic, phialidic, ampulliform, yellowish to pale brown, aseptate, smooth-walled. Conidia 3–5 × 2–3 μm (x̄ = 4 × 2.5 μm, n = 30), solitary, globose or oblong to ellipsoid, rounded or obtuse ends, yellowish to pale brown, aseptate, rarely guttulated, one-celled, smooth-walled.

Figure 2.

Corylicola italica sp. nov. (MFLU 19–0500, holotype). a–b. appearance of ascomata on a twig of Corylus avellana; c. Longitudinal section of ascomata; d. ascoma neck and ascospore mass (arrowed); e peridium wall; f pseudoparaphyses; g–j. asci; k–l. ascospores; m. germinated ascospore; n–o. culture characteristics on PDA (n = from above, o = from below) Scale bars: a = 200 μm, b–c = 100 μm, d, f–j = 20 μm, e, k–m = 5 μm.

Figure 3.

Asexual morph of Corylicola italica sp. nov. on PDA (MFLUCC 20–0111, ex-type). a. conidiomata on PDA; b. vegetative hyphae on agar media; c. longitudinal section of conidiomata; d. conidioma wall; e–h. conidiogenous cells; i–l. conidia. Scale bars: a = 200 μm, b–c = 20 μm, d = 10 μm, e–l = 5 μm.

Culture characteristics:

Ascospores germinating on PDA within 24 hours from single-spore isolation. Colonies on PDA reaching 5–10 mm diam. after 14 days at 16°C, circular, crenated edge, flat with dense, whitish-grey in upper and brownish-black in the lower surface of the colony. Sporulated after 12 weeks.

Facesoffungi Number:

FoF 08685

Etymology

Referring to the country where the holotype was collected, Italy

Notes

Corylicola italica sp. nov. shows morphological characters that are similar to other representatives in the family Bambusicolaceae. Based on morphological comparison with the type species of other genera in the family, Corylicola italica is similar to Palmiascoma gregariascomum (MFLU 11–0211) in having uni-loculate ascomata, central ostioles with minute papilla, cellular pseudoparaphyses and single-septate, echinulate, brown ascospores (Liu et al. 2015). Corylicola italica has cylindrical asci with short, furcate pedicels similar to Bambusicola massarinia (MFLU 12–0405), while P. gregariascomum has clavate asci with short rounded to obtuse pedicels (Dai et al. 2012, Liu et al. 2015). The branching and anastomosing pseudoparaphyses above the asci of C. italica are similar to B. massarinia and P. gregariascomum. However, the absence of a mucilaginous sheath around the ascospores in C. italica distinguishes it from both B. massarinia and P. gregariascomum (Dai et al. 2012, Liu et al. 2015). In addition, B. massarinia has hyaline ascospores, whereas both C. italica and P. gregariascomum have yellowish-brown ascospores (Dai et al. 2012, Liu et al. 2015).

The asexual state of C. italica (Fig. 3) is similar to P. gregariascomum (MFLUCC 11–0175) and Leucaenicola aseptata (MFLUCC 17–2423) in having pycnidial, globose to subglobose and glabrous conidiomata with a central ostiole and similar structures of conidiomata walls (outer layers; textura angularis to textura prismatica, inner layers; textura angularis) (Liu et al. 2015, Jayasiri et al. 2019). However, C. italica differs from these species in having globose conidia that are rarely guttulate, rather than oblong or ellipsoidal (Liu et al. 2015, Jayasiri et al. 2019). Bambusicola massarinia (MFLUCC 11–0389) is different from C. italica in having cylindrical conidia. Leucaenicola aseptata (MFLU 17–2423) distinguishes itself, based on its enteroblastic conidiogenous cells (Dai et al. 2012, Jayasiri et al. 2019). Corylicola italica and P. gregariascomum (MFLUCC 11–0175) both have uni-loculate to multi-loculate conidiomata (Liu et al. 2015). However, C. italica has ampulliform conidiogenous cells, whereas P. gregariascomum (MFLUCC 11–0175) has cylindrical conidiogenous cells instead (Liu et al. 2015). These morphological differences of the sexual (Fig. 2) and asexual (Fig. 3) morphs of Corylicola italica compared to other Bambusicolaceae species, in combination with the results of our multi-locus phylogenetic analysis, allow us to establish Corylicola as a new genus in Bambusicolaceae with C. italica as its type species.

Identification keys

Key to genera in Bambusicolaceae
1	Sexual and asexual morph known	2
–	Only asexual morph known	*Leucaenicola*
2	Yellowish-brown and 1-septate ascospores	3
–	Hyaline and 1–3 septate ascospores	*Bambusicola*
3	Cylindrical asci with short furcate pedicel	*Corylicola*
–	Clavate asci with short rounded to obtuse pedicel	*Palmiascoma*

Analysis

Phylogenetic analyses

DNA sequences derived from extractions from fruiting bodies were identical to those obtained from axenic mycelium. The final concatenated SSU, ITS, LSU, RPB2 and TEF1 alignment (Fig. 1) comprised 33 strains including the outgroup taxon Neoaquastroma guttulatum (MFLUCC 14–0917) and the manually adjusted dataset consisted of 4390 characters including gaps (SSU: 998, LSU: 832, ITS: 647, RPB2: 962, TEF1: 951), of which 857 were parsimony-informative. The ML tree topology is similar to the one of the BI consensus tree. Additionally, the tree topology is similar to previous work Yang et al. (2019). The two strains of Corylicola italica (MFLUCC 20–0111, MFLU 19–0500) grouped together with maximum support (B, Fig. 1) and formed a distinct lineage within Bambusicolaceae, placed sister to Bambusicola with moderate support (71% ML/1.00 PP, Fig. 1). The best-scoring RAxML tree (-lnL = 20609.761363) is shown in Fig. 1.

Discussion

In our multi-locus phylogenetic analysis (Fig. 1), we represent the recognised genera in Bambusicolaceae by letters A, B, C and D. The new genus Corylicola, with a single species C. italica (B), is phylogenetically distinct from other genera in Bambusicolaceae (A, C and D). It is positioned as sister to Bambusicola (A). Nucleotide differences of the ex-type strain of C. italica (MFLUCC 20–0111) were compared against the type species of other genera in Bambusicolaceae. Number of nucleotide differences (including gaps) by gene region were as follows: for Bambusicola massarinia: ITS (JX442033), 57/455 bp different (12.52%); LSU (JX442037), 22/830 bp different (2.65%); RPB2 (KP761716), 144/960 bp different (15%); TEF1 (KP761725), 53/920 bp different (5.7%): for Palmiascoma gregariascomum: ITS (KP744452), 40/455 bp different (8.79%); LSU (KP744495), 21/831 bp different (2.52%); RPB2 (KP998466), 165/956 bp different (17.25%). These numbers further confirm taxonomic placement of the new genus.

Bamboo is a medicinal plant in which saprobic microfungi are abundant on culms and leaves (Dai et al. 2018, Rathnayaka et al. 2019, Sun et al. 2020). Most species of Bambusicola are reported on dead culms of bamboos (Dai et al. 2012, Dai et al. 2015, Dai et al. 2017, Liu et al. 2015, Thambugala et al. 2017, Jayasiri et al. 2019). Recently, Yang et al. (2019) introduced Bambusicola subthailandica and B. sichuanensis from Phyllostachys heteroclada, showing these species could be distributed on a wide range of hosts. Leucaenicola species were isolated from decaying pods of Leucaena sp. (Jayasiri et al. 2019) and leaf spots of Osmanthus fragrans (Ariyawansa et al. 2020). Palmiascoma species were identified from dead palm frond and dead branches of Eucalyptus sp. (Liu et al. 2015, Jayasiri et al. 2019). Most species have been isolated from Thailand and a few from China and Taiwan (Yang et al. 2019, Ariyawansa et al. 2020). Corylicola italica gen. & sp. nov. is the first reported Bambusicolaceae species from Corylus avellana (Fagales) in Italy. Whereas Bambusicola, Corylicola and Palmiascoma have both sexual and asexual morphs, Leucaenicola has only asexual morphs.

Acknowledgements

Yong Wang would like to thank the National Natural Science Foundation of China (No. 31972222, 31560489), Program of Introducing Talents of Discipline to Universities of China (111 Program, D20023), Science and Technology basic work of MOST [2014FY120100], Talent project of Guizhou Science and Technology Cooperation Platform ([2017]5788-5 and [2019]5641) and Guizhou Science, Technology Department International Cooperation Basic project ([2018]5806). S.N. Wijesinghe would like to thank Mae Fah Luang University for financial support and S.C. Karunarathna, R.G.U. Jayalal, A.R. Rathnayaka, A.G. Niego and D. Bundhun for their precious assistance during this study. D.N. Wanasinghe would like to thank the CAS President’s International Fellowship Initiative (PIFI) for funding his postdoctoral research (number 2019PC0008), the National Science Foundation of China and the Chinese Academy of Sciences for financial support under the following grants: 41761144055, 41771063 and Y4ZK111B01. K.D. Hyde would like to thank the Thailand Research Fund (“Impact of climate change on fungal diversity and biogeography in the Greater Mekong Sub-region RDG6130001”).

Author contributions

References

Ariyawansa HA, Tsai I, Hozzein WN, Thambugala KM (2020)

Leucaenicola osmanthi sp. nov. (Bambusicolaceae, Pleosporales), causing leaf spot of Osmanthus fragrans in Taiwan.

Phytotaxa

437

‑

. https://doi.org/10.11646/phytotaxa.437.1.3

Chomnunti P, Hongsanan S, Hudson BA, Tian Q, Peroh D, Dhami MK, Alias AS, Xu J, Liu X, Stadler M, Hyde KD (2014)

The sooty moulds

Fungal Diversity

‑

. https://doi.org/10.1007/s13225-014-0278-5

Crous PW, Wingfield MJ, Schumacher RK, Summerell BA, Giraldo A, Gen J, Guarro J, Wanasinghe DN, Hyde KD, Camporesi E, Gareth Jones EB, Thambugala KM, Malysheva EF, Malysheva VF, Acharya K, Álvarez J, Alvarado P, Assefa A, Barnes CW, Bartlett JS, Blanchette RA, Burgess TI, Carlavilla JR, Coetzee MP, Damm U, Decock CA, den Breeÿen A, de Vries B, Dutta AK, Holdom DG, Rooney-Latham S, Manjón JL, Marincowitz S, Mirabolfathy M, Moreno G, Nakashima C, Papizadeh M, Shahzadeh Fazeli SA, Amoozegar MA, Romberg MK, Shivas RG, Stalpers JA, Stielow B, Stukely MJ, Swart WJ, Tan YP, van der Bank M, Wood AR, Zhang Y, Groenewald JZ (2014)

Fungal plant description sheets: 281319

Persoonia

212

‑

289

. https://doi.org/10.3767/003158514X685680

Dai D, Tang L, Wang H (2018)

A review of bambusicolous ascomycetes

. In: Abdul Khalil H (Ed.)

Bamboo: Current and Future Prospects

165-183

pp. [ISBN

978-1-78923-231-8

]. https://doi.org/10.5772/intechopen.76463

Dai DQ, Bhat DJ, Liu JK, Chukeatirote E, Zhao R, Hyde KD (2012)

Bambusicola, a new genus from bamboo with asexual and sexual morphs.

Cryptogamie, Mycologie

363

‑

379

. https://doi.org/10.7872/crym.v33.iss3.2012.363

Dai DQ, Bahkali AH, Li WJ, Bhat DJ, Zhao RL, Hyde KD (2015)

Bambusicola loculata sp. nov. (Bambusicolaceae) from bamboo

Phytotaxa

213

122

‑

130

. https://doi.org/10.11646/phytotaxa.213.2.5

Dai DQ, Phookamsak R, Wijayawardene NN, Li WJ, Bhat DJ, Xu JC, Taylor JE, Hyde KD, Chukeatirote E (2017)

Bambusicolous fungi

Fungal Diversity

‑

105

. https://doi.org/10.1007/s13225-016-0367-8

Glez-Pea D, Gmez-Blanco D, Reboiro-Jato M, Fdez-Riverola F, Posada D (2010)

ALTER: program oriented conversion of DNA and protein alignments

Nucleic Acids Research

‑

. https://doi.org/10.1093/nar/gkq321

Hall TA (1999)

BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT

Nucleic Acids Symposium Series

‑

Hopple JS (1994)

Phylogenetic investigations in the genus Coprinus based on morphological and molecular characters

PhD thesis, Duke University

Durham, NC

Hyde KD, Jones EB, Liu JK, Ariyawansha H, Boehm E, Boonmee S, Braun U, Chomnunti P, Crous P, Dai DQ, Diederich P, Dissanayake A, Doilom M, Doveri F, Hongsanan S, Jayawardena R, Lawrey JD, Li YM, Liu YX, Lcking R, Monkai J, Nelsen MP, Phookamsak R, Muggia L, Pang KL, Senanayake I, Shearer CA, Wijayawardene N, Wu HX, Thambugala M, Suetrong S, Tanaka K, Wikee S, Zhang Y, Hudson BA, Alias SA, Aptroot A, Bahkali AH, Bezerra LJ, Bhat JD, Camporesi E, Chukeatirote E, Hoog SD, Gueidan C, Hawksworth DL, Hirayama K, Kang JC, Knudsen K, Li WJ, Liu ZY, McKenzie EH, Miller AN, Nadeeshan D, Phillip AJ, Mapook A, Raja HA, Tian Q, Zhang M, Scheuer C, Schumm F, Taylor J, Yacharoen S, Tibpromma S, Wang Y, Yan J, Li X (2013)

Families of Dothideomycetes

Fungal Diversity

‑

313

. https://doi.org/10.1007/s13225-013-0263-4

Index Fungorum (2020) http://www.indexfungorum.org/Names/Names.asp. Accessed on: 2020-5-10.

Jayasiri SC, Hyde KD, Ariyawansa HA, Bhat DJ, Buyck B, Cai L, Dai YC, Abd-Elsalam KA, Ertz D, Hidayat I, Jeewon R, Jones EB, Bahkali AH, Karunarathna SC, Liu JK, Luangsa-ard JJ, Lumbsch HT, Maharachchikumbura SS, McKenzie EH, Moncalvo JM, Ghobad-Nejhad M, Nilsson H, Pang KL, Pereira OL, Phillips AJ, Rasp O, Rollins AW, Romero AI, Etayo J, Seluk F, Stephenson SL, Suetrong S, Taylor JE, Tsui CK, Vizzini A, Abdel-Wahab MA, Wen TC, Boonmee S, Dai DQ, Daranagama DA, Dissanayake AJ, Ekanayaka AH, Fryar SC, Hongsanan S, Jayawardena RS, Li WJ, Perera RH, Phookamsak R, Silva NI, Thambugala KM, Tian Q, Wijayawardene NN, Zhao RL, Zhao Q, Kang JC, Promputtha I (2015)

The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts

Fungal Diversity

‑

. https://doi.org/10.1007/s13225-015-0351-8

Jayasiri SC, Hyde KD, Jones EB, McKenzie EH, Jeewon R, Phillips AJ, Bhat DJ, Wanasinghe DN, Liu JK, Lu YZ, Kang JC (2019)

Diversity, morphology and molecular phylogeny of Dothideomycetes on decaying wild seed pods and fruits

Mycosphere

‑

186

. https://doi.org/10.5943/mycosphere/10/1/1

Katoh K, Standley DM (2013)

MAFFT multiple sequence alignment software version 7: improvements in performance and usability

Molecular Biology and Evolution

772

‑

780

. https://doi.org/10.1093/molbev/mst010

Katoh K, Rozewicki J, Yamada KD (2017)

MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization

Briefings in Bioinformatics

1160

‑

1166

. https://doi.org/10.1093/bib/bbx108

Liu JK, Hyde KD, Jones EB, Ariyawansa HA, Bhat JD, Boonmee S, Maharachchikumbura SS, Mckenzie EH, Phookamsak R, Phukhamsakda C, Shenoy BD, Abdel-Wahab MA, Buyck B, Chen J, Chethana KW, Singtripop C, Dai DQ, Dai YC, Daranagama DA, Dissanayake AJ, Doilom M, Dsouza MJ, Fan XL, Goonasekara ID, Hirayama K, Hongsanan S, Jayasiri SC, Jayawardena RS, Karunarathana SC, Li WJ, Mapook A, Norphanphoun C, Pang KL, Perera RH, Peroh D, Pinruan U, Senanayake IC, Somrithipol S, Suetrong S, Tanaka K, Thambugala KM, Tian Q, Tibpromma S, Udayanga D, Wuayawardene NN, Wanasinghe D, Wisitrassameewong K, Zeng XY, Abdel-Aziz FA, Adamk S, Bahkali AH, Boonyuen N, Bulgakov T, Callac P, Chomnunti P, Greiner K, Hashimoto A, Hofstetter V, Kang JC, Lewis D, Li XL, Liu X, Liu ZY, Matsumura M, Mortimer PE, Rambold G, Randrianjohany E, Sato G, Sriindrasutdhi V, Tian CM, Verbeken A, Von Brackel W, Wang Y, Wen TC XJYJZRCE, Xu JC, Yan JY, Zhao RL, Camporesi E (2015)

Fungal diversity notes 1110: taxonomic and phylogenetic contributions to fungal species

Fungal Diversity

‑

197

. https://doi.org/10.1007/s13225-015-0324-y

Liu Y, Whelen S, BD H (1999)

Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit.

Molecular Biology and Evolution

1799

‑

1808

. https://doi.org/10.1093/oxfordjournals.molbev.a026092

Miller M, Pfeiffer W, T S (2012)

The CIPRES science gateway: enabling high-impact science for phylogenetics researchers with limited resources

Proceedings of the 1st Conference of the Extreme Science and Engineering Discovery Environment: Bridging from the extreme to the campus and beyond (XSEDE ’12)

Association for Computing Machinery, USA

1-8

pp. https://doi.org/10.1145/2335755.2335836

Nitride C, Picariello G, Mamone G, Ferranti P (2017)

Proteomics of Hazelnut (Corylus avellana)

. In: Colgrave M (Ed.)

Proteomics in Food Science

538

pp. [In

English

]. [ISBN

978-0-12-804007-2

]. https://doi.org/10.1016/B978-0-12-804007-2.00007-2

Nylander J (2004)

MrModeltest 2.0

Program distributed by the author

Evolutionary Biology Centre, Uppsala University

Phukhamsakda C, Hongsanan S, Ryberg M, Ariyawansa HA, Chomnunti P, Bahkali AH, Hyde KD (2016)

The evolution of Massarineae with Longipedicellataceae fam. nov

Mycosphere

1713

‑

1731

. https://doi.org/10.5943/mycosphere/7/11/7

Rambaut A, Drummond A (2007)

Tracer

v1, 4

. URL: http://beast.bio.ed.ac.uk/Tracer

Rambaut A (2012)

FigTree

v. 1.4.0

. URL: http://tree.bio.ed.ac.uk/ software/figtree/

Rathnayaka AR, Dayarathne MC, Maharachchikumbura SS, Liu JK, Tennakoon DS, Hyde KD (2019)

Introducing Seriascoma yunnanense sp. nov. (Occultibambusaceae, Pleosporales) based on evidence from morphology and phylogeny

Asian Journal of Mycology

245

‑

253

. https://doi.org/10.5943/ajom/2/1/15

Rehner S (2001)

Primers for elongation factor 1-α (EF1-α)

. URL: http://ocid. nacse.org/research/deephyphae/EF1primer.pdf

Rehner SA, Samuels GJ (1994)

Taxonomy and phylogeny of Gliocladium analyzed from nuclear large subunit ribosomal DNA sequences

Mycological Research

625

‑

634

. https://doi.org/10.1016/S0953-7562(09)80409-7

Stamatakis A (2014)

RAxML Version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies

Bioinformatics

1312

‑

1313

. https://doi.org/10.1093/bioinformatics/btu033

Sun Y, Goonasekara I, Thambugala K, Jayawardena R, Wang Y, Hyde K (2020)

Distoseptispora bambusae sp. nov. (Distoseptisporaceae) on bamboo from China and Thailand

Biodiversity Data Journal

e53678

. https://doi.org/10.3897/BDJ.8.e53678

Swofford DL (2003)

PAUP* 4.0b10: phylogenetic analysis using parsimony (*and other methods)

Sinauer Associates, Sunderland, Massachusetts

Tanaka K, Hirayama K, Yonezawa H, Hatakeyama S, Harada Y, Sano T, Shirouzu T, Hosoya T (2009)

Molecular taxonomy of bambusicolous fungi: Tetraplosphaeriaceae, a new pleosporalean family with Tetraploa-like anamorphs

Studies in Mycology

175

‑

209

. https://doi.org/10.3114/sim.2009.64.10

Tanaka K, Hirayama K, Yonezawa H, Sato G, Toriyabe A, H K, Hashimoto A, Matsumura M, Harada Y, Kurihara Y, Shirouzu T, Hosoya T (2015)

Revision of the Massarineae (Pleosporales, Dothideomycetes)

Studies in Mycology

‑

136

. https://doi.org/10.1016/j.simyco.2015.10.002

Thambugala KM, Wanasinghe DN, Phillips AJ, Camporesi E, Bulgakov TS, Phukhamsakda C, Ariyawansa HA, Goonasekara ID, Phookamsak R, Dissanayake A, Tennakoon DS, Tibpromma S, Chen YY, Liu ZY, Hyde KD (2017)

Mycosphere notes 150: grass (Poaceae) inhabiting Dothideomycetes

Mycosphere

697

‑

796

. https://doi.org/10.5943/mycosphere/8/4/13

Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997)

The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools

Nucleic Acids Research

4876

‑

4882

. https://doi.org/10.1093/nar/25.24.4876

Vilgalys R, Hester M (1990)

Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species

Journal of Bacteriology

172

4238

‑

4246

. https://doi.org/10.1128/jb.172.8.4238-4246.1990

White TJ, Bruns T, Lee S, Taylor J (1990)

Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies.

In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds)

PCR protocols: a guide to methods and applications.

Academic Press

Academic Press, San Diego

315-322

pp. https://doi.org/10.1016/B978-0-12-372180-8.50042-1

Wijayawardene N, Hyde K, Rajeshkumar K, Hawksworth D, Madrid H, Kirk P, Braun U, Singh R, Crous P, Kukwa M, Lücking R, Kurtzman C, Yurkov A, Haelewaters D, Aptroot A, Lumbsch HT, Timdal E, Ertz D, Etayo J, Phillips AL, Groenewald J, Papizadeh M, Selbmann L, Dayarathne M, Weerakoon G, Jones EBG, Suetrong S, Tian Q, Castañeda-Ruiz R, Bahkali A, Pang K, Tanaka K, Dai DQ, Sakayaroj J, Hujslová M, Lombard L, Shenoy B, Suija A, Maharachchikumbura SN, Thambugala K, Wanasinghe D, Sharma B, Gaikwad S, Pandit G, Zucconi L, Onofri S, Egidi E, Raja H, Kodsueb R, Cáceres MS, Pérez-Ortega S, Fiuza P, Monteiro JS, Vasilyeva L, Shivas R, Prieto M, Wedin M, Olariaga I, Lateef AA, Agrawal Y, Fazeli SAS, Amoozegar MA, Zhao GZ, Pfliegler W, Sharma G, Oset M, Abdel-Wahab M, Takamatsu S, Bensch K, de Silva NI, De Kesel A, Karunarathna A, Boonmee S, Pfister D, Lu Y, Luo Z, Boonyuen N, Daranagama D, Senanayake I, Jayasiri S, Samarakoon M, Zeng X, Doilom M, Quijada L, Rampadarath S, Heredia G, Dissanayake A, Jayawardana R, Perera R, Tang LZ, Phukhamsakda C, Hernández-Restrepo M, Ma X, Tibpromma S, Gusmao LP, Weerahewa D, Karunarathna S (2017)

Notes for genera: Ascomycota

Fungal Diversity

(

‑

594

. https://doi.org/10.1007/s13225-017-0386-0

Wijayawardene NN, Hyde KD, Al-Ani LK, Tedersoo L, Haelewaters D, Rajeshkumar KC, Zhao RL, Aptroot A, Leontyev DV, Saxena RK, Tokarev YS, Dai DQ, Letcher PM, Stephenson SL, Ertz D, Lumbsch HT, Kukwa M, Issi IV, Madrid H, Phillips AJL SL, Selbmann L, Pfliegler WP, Horváth E, Bensch K, Kirk P, Kolaříková Z, Raja HA, Radek R, Papp V, Dima B, Ma J, Malosso E, Takamatsu S, Rambold G, Gannibal PB, Triebel D, Gautam AK, Avasthi S, Suetrong S, Timdal E, Fryar SC, Delgado G, Réblová M, Doilom M, Dolatabadi S, Pawłowska J, Humber RA, Kodsueb R, SánchezCastro I, Goto BT, Silva DKA, De Souza FA, Oehl F, Da Silva GA, Silva IR, Błaszkowski J, Jobim K, Maia LC, Barbosa FR, Fiuza PO, Divakar PK, Shenoy BD, Castañeda-Ruiz RF, Somrithipol S, Karunarathna SC, Tibpromma S, Mortimer PE, Wanasinghe DN, Phookamsak R, Xu J, Wang Y, Fenghua T, Alvarado P, Li DW, Kušan I, Matočec N, Maharachchikumbura SSN, Papizadeh M, Heredia G, Wartchow F, Bakhshi M, Boehm E, Youssef N, Hustad VP, Lawrey JD, Santiago ALCMA, Bezerra JDP, Souza-Motta CM, Firmino AL, Tian Q, Houbraken J, Hongsanan S, Tanaka K, Dissanayake AJ, Monteiro JS, Grossart HP, Suija A, Weerakoon G, Etayo J, Tsurykau A, Kuhnert E, Vázquez V, Mungai P, Damm U, Li QR, Zhang H, Boonmee S, Lu YZ, Becerra AG, Kendrick B, Brearley FQ, Motiejűnaitë J, Sharma B, Khare R, Gaikwad S, Wijesundara DSA, Tang LZ, He MQ, Flakus A, Rodriguez-Flakus P, Zhurbenko MP, McKenzie EHC, Stadler M, Bhat DJ, Liu JK, Raza M, Jeewon R, Nassonova ES, Prieto M, Jayalal RGU, Yurkov A, Schnittler M, Shchepin ON, Novozhilov YK, Liu P, Cavender JC, Kang Y, Mohammad S, Zhang LF, Xu RF, Li YM, Dayarathne MC, Ekanayaka AH, Wen TC, Deng CY, Lateef AA, Pereira OL, Navathe S, Hawksworth DL, Fan XL, Dissanayake LS, Erdoðdu M (2020)

Outline of Fungi and fungi-like taxa

Mycosphere

1060

‑

1456

. https://doi.org/10.5943/mycosphere/11/1/8

Wijesinghe SN, Dayarathne MC, Maharachchikumbura SS, Wanasinghe DN, Hyde KD (2019)

Ceramothyrium chiangraiense, a novel species of Chaetothyriales (Eurotiomycetes) from Ficus sp. in Thailand

Asian Journal of Mycology

269

‑

280

. https://doi.org/10.5943/ajom/2/1/17

Yang CL, Xu XL, Liu YG (2019)

Two new species of Bambusicola (Bambusicolaceae, Pleosporales) on Phyllostachys heteroclada from Sichuan, China

Nova Hedwigia

108

527

‑

545

. https://doi.org/10.1127/nova_hedwigia/2019/0526

Zhang H, Dong W, Hyde KD, Bahkali AH, Liu JK, Zhou D, Zhang DI (2016)

Molecular data shows Didymella aptrootii is a new genus in Bambusicolaceae

Phytotaxa

247

‑

108

. https://doi.org/10.11646/phytotaxa.247.2.1

Zhang Y, Wang HK, Fournier J, Crous PW, Jeewon R, Pointing SB, Hyde KD (2009)

Towards a phylogenetic clarification of Lophiostoma /Massarina and morphologically similar genera in the Pleosporales

Fungal Diversity

225

‑

251

Zhang Y, Crous PW, Schoch CL, Hyde KD (2012)

Pleosporales

Fungal Diversity

‑

221

. https://doi.org/10.1007/s13225-011-0117-x

Supplementary material

Endnotes