Biodiversity Data Journal :
Taxonomic Paper
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Corresponding author: Yony T. Callohuari (ycallohuari@lamolina.edu.pe)
Academic editor: Ľubomír Kováč
Received: 18 Aug 2020 | Accepted: 15 Nov 2020 | Published: 25 Nov 2020
© 2020 José G. Palacios-Vargas, Yony T. Callohuari
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Palacios-Vargas JG, Callohuari YT (2020) A new species of the genus Neotropiella Handschin, 1942 (Collembola: Neanuridae) from Peru. Biodiversity Data Journal 8: e57743. https://doi.org/10.3897/BDJ.8.e57743
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Neotropiella is a genus of springtails which can be of medium size (2 mm) or relatively long (5 mm). These springtails live in leaf litter, under the bark of dead trees or in decomposing wood, mainly in the Neotropical Region and are often collected by litter samples on Berlese funnels or by pitfall traps. Most species have been described, based on relatively few specimens and chaetotaxy of several species is incomplete.
A new species within Neotropiella was discovered in recent pitfall trap collections from Peru. Neotropiella peruana sp. n. was taxonomically treated and studied under both phase contrast and scanning electron microscopy. It is similar to N. insularis from Brazil, but smaller with only 4 mandibular teeth (vs. 5) and with well-developed unguis lateral teeth. Intraspecific variation of the new species is provided. We also present the first DNA barcodes for the genus.
taxonomy, chaetotaxy, Pseudachorutinae, Neotropics, Neanuridae.
Members of the genus Neotropiella are often abundant in forest litter, but are also found in decomposing wood, bark and moss on branches of trees and even in epiphytic plants. They are often collected by Malaise traps, canopy fogging and by pitfall traps. The genus in mainly distributed in the Neotropical Region where 18 species have been listed (
The only species of this genus cited from Peru is Neotropiella carli (Denis, 1924) which can measure up to 5 mm long; the new species here described is less than half the size and has a stronger pigmentation and several different characters. It was collected by pitfall traps at San Ramón, Chanchamayo, fundo Génova. The locus typicus is Yunga lower montane forest (Fig.
Material from Peru was collected by the second author using pitfall traps and preserved in 96% ethanol. A total of 19 specimens were subsequently slide-mounted in Hoyers’ solution at Laboratorio de Ecología y Sistemática de Microartrópodos, Faculty of Sciences, Universidad Nacional Autónoma de México for study.
For the Scanning Electron Microscopy (SEM) study, several specimens were dehydrated using a graduated series of ethanol dilutions, then dried using a critical point dryer (Baltec CPD030) and coated in gold using a Denton Vacuum Desk II ioniser.
For the molecular study, five specimens were photographed and sent for sequencing with the standard COI–5P marker (“DNA barcode”,
For the morphological study, specimens or cuticles were mounted in Hoyer’s solution after clearing the specimens. Some slides were remounted to study chaetotaxy and morphological variation.
Nineteen specimens (including vouchers and type material) were deposited in the following collections:
LESM Laboratorio de Ecología y Sistemática de Microartrópodos, Universidad Nacional Autónoma de Mexico, Ciudad de México, México.
MEKRB Museo de Entomología Klaus Raven Büller, Universidad Nacional Agraria La Molina, La Molina, Lima, Perú.
A-G—labial setae of median area; Abd—abdominal segment; Ant—antennal segment; cf—cuticular fold of sensorial Ant III organ; Cx—coxa; d-f—lateral labial setae; d1-5—dorsal cephalic setae; De—dorso external area; Di—dorso-internal area; DL—Dorso-lateral area; Fe—femur; g—genal seta 4,5; hr—anal valve setae; i— dorsal microseta “i” of Ant IV; L—vestigial tuberculate labial seta L; Lb—labium; Lr— labrum; M—Median seta of tibiotarsus proximal verticile Ma—macroseta; m—microseta; m’—ventral or lateral microsensillum; m—microsetae; ms— microsensillum; Oc1, Oc2, Oc3—ocular setae 1, 2, 3; Mx—maxilla; PAO—postantennal organ; or— antenal organite; S—antennal sensillum 1-9 sensilla; Scx I—subcoxa I; Scx II—subcoxa II; sd—subdorsal seta 1-4; sf—ventral sensorial field of Ant IV; Sgd—dorsal guard sensillum; Sgv—ventral guard sensillum; ss—body sensorial setae; Tita—tibiotarsus; Th—thoracic segment; Tr—trochanter; VT—ventral tube. Setae: a—anterior row of setae; m—median row of setae; p—posterior row of setae.
Class Collembola Lubbock, 1870
Order Poduromorpha Börner, 1913
Family Neanuridae Börner, 1901
Neotropiella Handchin, 1942 in
Type species: Neotropiella silvestrii (Denis, 1929) from Cuba (
Body length average (n = 14) 1.6 mm (range 1.25-2.25 mm). Colour in ethanol (Fig.
Ratio head diagonal (275 µm): antenna (300 µm) = 1:1.3. Ant I (64 µm) with 9 setae, Ant II (80 µm) with 12 setae, Ant III and IV (Fig.
Eyes 5+5 on a black eye patch (Fig.
Dorsal chaetotaxy with heterochaetosis (Figs
The name is locative, after of the country where it was collected in Peru.
DNA was successfully obtained from four specimens of N. peruana sp. n., sequences are BCICL099-19, BCICL101-19, BCICL102-19 and BCICL103-19, which were deposited in the project BCICL of the Barcode of Life Data System (http://www.barcodinglife.org/index.php). The Barcode Index Number for this species is BOLD:ADS2464 (hyperlink activated as https://www.boldsystems.org/index.php/Public_BarcodeCluster?clusteruri=BOLD:ADS2464).
The cuticles of four specimens were recovered and mounted in Hoyer’s solution which represent the vouchers and are kept at the author’s institution.
Neotropiella peruana sp. nov. is characterised by the combination of a ventral sensorial field on Ant IV with about 100 modified setae, small body size (1.2-2.2 mm) and small number of vesicles in the postantennal organ (26-28 in average). Mandibles have 4 teeth and maxillae with 2 lamella, 1 acuminate and 1 hooked. Th I with 4 pairs of smooth microsetae and Di area on Th II and III with 3 setae. 1 + 1 lateral tooth on unguis besides the inner 1 and often 1 + 1 subapical tooth.
N. peruana sp. nov. shares with N. carli (
Variation: For body length, only 14 specimens were measured (adults and juveniles) from the same locality. The average is 1.665 µm (range 1.250-2.250 µm). Ant IV sensilla have usually 6, but few specimens had 7-8 or even 5. Ant IV ventral file, from 65 to 116 modified setae (average 87). Eyes are mostly constant, 5 + 5, but in one case, there was one specimen with 5 + 4 eyes. PAO, minimum vesicles 20, maximum 53 vesicles, average 26-28. Mandibles always with 4 teeth. Maxilla always hooked. Labium always the same pattern, only one specimen had 0 + 1 postlabial seta. Unguis was studied in 17 specimens; they always had one inner tooth and 2 lateral teeth, but in 7 cases, there was one pair of subapical small teeth as shown in Figure 8. Head chaetotaxy seems to be constant. Body chaetotaxy variation was studied in 14 well preserved specimens, but only on Th. I and Abd. V was strong variation found. The seta m1 of Th I is always microseta (14 cases); m2 as microseta in 3 cases and as meso- or macroseta in 11 cases; m3 as microseta in 4 cases and meso- or macroseta in 8 cases; m4 is always microseta; lateral seta is always microseta. On Abd V, the seta p1 is always microseta; p3 is always the sensorial seta, p4 is always a microseta, seta p5 is microseta, in one case, it was macroseta, lateral seta p6 is always microseta. One specimen has one teratological antenna, where only the basal segment was present and at the tip one large sensillum, another has one teratology on leg II where the tibiotarsus was lacking. One female presents only 1 eugenital seta instead of 2 such setae.
FONDECYT-Perú provided financial support to Yony T. Callohuari for traveling to UNAM, México to work on Collembola. Additional specimens from Peru were donated by Reyna Zuasnabar. SEM photographs were taken by Silvia Espinosa, from Laboratorio de Microscopía Electrónica de Barrido, Facultad de Ciencias, UNAM. Angela Arango and Maira Montejo took the pictures of the habitus. Elihú Catalán prepared some of the drawings under the phase contrast microscope and Jair Páez prepared the final plates. Erick García (in charge of the Science Faculty collections) assigned the catalogue number. The molecular part of this contribution was possible thanks to the project “Application of metabarcoding tools to long-term monitoring of soil fauna” (Yves Basset & Héctor Barrios), funded by SENACYT FID16-070. A sincere thank you to Dr. Nicholas Seiter for his diligent proofreading of this paper.