Biodiversity Data Journal :
Single Taxon Treatment
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Corresponding author: Héctor A. Vargas (lepvargas@gmail.com)
Academic editor: Shinichi Nakahara
Received: 27 Oct 2021 | Accepted: 19 Nov 2021 | Published: 24 Nov 2021
© 2021 Héctor Vargas
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Vargas HA (2021) New distribution records, first host plant record and DNA barcoding of the Neotropical plume moth Oidaematophorus pseudotrachyphloeus Gielis (Lepidoptera, Pterophoridae). Biodiversity Data Journal 9: e77167. https://doi.org/10.3897/BDJ.9.e77167
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Oidaematophorus pseudotrachyphloeus Gielis, 2011 (Lepidoptera, Pterophoridae) is a little-known Neotropical plume moth previously recorded in Ecuador, Peru and Argentina. Its host plants and DNA barcodes are unknown.
Oidaematophorus pseudotrachyphloeus is recorded for the first time from Chile, based on six specimens from the Azapa Valley (Arica Province) and two from Guayacán (Coquimbo Province). Those from the Azapa Valley were reared from folivorous larvae collected on Ambrosia cumanensis Kunth (Asteraceae), representing the first host plant record for this plume moth. The first DNA barcode sequences of O. pseudotrachyphloeus are provided and used to explore relationships with congenerics.
Asteraceae, Atacama Desert, Chile, folivorous larvae, mitochondrial DNA
Oidaematophorus Wallengren, 1862 (Lepidoptera, Pterophoridae, Pterophorinae, Oidaematophorini) is a widespread genus of plume moths with 24 species described (
Six species of Oidaematophorus were listed by
Hitherto, O. pseudotrachyphloeus Gielis, 2011 is reported in Ecuador, Peru and Argentina (
Plume moths were reared from larvae collected on Ambrosia cumanensis Kunth (Asteraceae) in the Azapa Valley, Arica Province, northern Chile. Eight larvae were collected in July 2017 and placed in plastic vials with leaves of A. cumanensis and a paper towel at the bottom. Additional leaves were provided until the larvae completed feeding and pupated, about ten days after collection. Before pupation, two larvae were placed in 95% ethanol at -20°C until DNA extraction. Adults emerged about twelve days after pupation in August 2017. A search for additional conspecifics from Chile was performed in the Colección Entomológica de la Universidad de Tarapacá (IDEA), Arica, Chile. The abdomen of each specimen was removed and placed in 10% potassium hydroxide (KOH) for a few minutes for genitalia dissection, stained with Chlorazol black and Eosin and mounted on slides with Euparal. Images of the genitalia were captured with a Sony CyberShot DSC-HX200V digital camera attached to a Leica M125 stereomicroscope. The identification of the specimens was based on comparisons with the original description of O. pseudotrachyphloeus provided by
Genomic DNA was extracted from two larvae following the procedures described in
Species |
BOLD accession |
GenBank accession |
Country |
Oidaematophorus balsamorrhizae McDunnough, 1939 |
USA |
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Oidaematophorus brucei (Fernald, 1898) |
USA |
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Oidaematophorus cineraceus (Fish, 1881) |
USA |
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Oidaematophorus giganteus (Mann, 1855) |
France |
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Oidaematophorus grisescens (Walsingham, 1880) |
USA |
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Oidaematophorus guttatus (Walsingham, 1880) |
USA |
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Oidaematophorus lindseyi McDunnough, 1923 |
Canada |
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Oidaematophorus lithodactyla (Treitschke, 1833) |
Italy |
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Oidaematophorus mathewianus (Zeller, 1874) |
Canada |
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Oidaematophorus pseudotrachyphloeus Gielis, 2011 |
Chile |
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Oidaematophorus rogenhoferi (Mann, 1871) |
Russia |
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Oidaematophorus vafradactylus Svensson, 1966 |
Estonia |
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Adaina microdactyla (Hübner, [1813]) |
Austria |
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Emmelina monodactyla Linneaus, 1758 |
Germany |
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Hellinsia osteodactylus (Zeller, 1841) |
Austria |
Six adults (three females, three males) of O. pseudotrachyphloeus (Fig.
Two identical sequences of O. pseudotrachyphloeus were obtained (GenBank accessions OK510535, OK510536), which represent the first DNA barcodes for this species. The lowest divergence (9.6% K2P) was with O. balsamorrhizae McDunnough, 1939 and O. cineraceus (Fish, 1881). Oidaematophorus was recovered as a monophyletic group in the ML analysis, but with low UFBoot support (Fig.
Based on previous records of O. pseudotrachyphloeus (
Ambrosia cumanensis, erroneously cited as A. peruviana Willd. in the botanical literature (
Although the ML analysis here presented is based on a single mitochondrial marker, it is promising that sequences of Oidaematophorus were clustered as a monophyletic group in agreement with the most recent morphological definition of the genus (
Eight species of Oidaematophorus were previously recorded from Chile (
The author thanks Maryzender Rodríguez-Melgarejo and Shinichi Nakahara for kind suggestions on a previous version of the manuscript, Bernard Landry and Juan Grados for sharing information and photos of the genitalia slide of the holotype of O. pseudotrachyphloeus, respectively, Marcelo Vargas-Ortiz for DNA extraction, Wilson Huanca-Mamani for the use of the molecular biology equipment and Lafayette Eaton for checking the English.