Biodiversity Data Journal :
Taxonomic Paper
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Corresponding author: Min Wang (minwang@scau.edu.cn)
Academic editor: Shinichi Nakahara
Received: 18 Nov 2021 | Accepted: 06 Feb 2022 | Published: 14 Feb 2022
© 2022 Yulong Zhang, Siyao Huang, Min Wang
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Zhang Y, Huang S, Wang M (2022) First record of the genus Olepa Watson, 1980 from China (Lepidoptera, Erebidae, Arctiinae, Arctiini). Biodiversity Data Journal 10: e78167. https://doi.org/10.3897/BDJ.10.e78167
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The tribe Arctiini is a species-rich tribe of the subfamily Arctiinae of the family Erebidae. The genus Olepa Watson, 1980 is distributed in the Oriental and Palearctic Regions and the diversity reaches its peak in south Asia.
We herein describe the first record of the genus Olepa from China and re-describe Olepa ricini (Fabricius, 1775), together with illustrations of its adult and male genitalia. Furthermore, based on an analysis of 658-bp COI barcoding sequences, together with morphological studies, we consider that Olepa schleini Witt et al., 2005 syn. n. is a new synonym of O. ricini.
Guangdong, Hainan, molecular analysis, venation, genitalia, synonym
The genus Olepa was originally established by
Morphological study. We photographed the adults using a Sony DSC-RX100 v1.00 camera. To study the wing venation, the wings were removed from the thorax and cleaned with a 1:1 mixture of bleaching liquid. A Nikon D750 camera was used to photograph the venation. Abdomens were removed and macerated in hot 10% sodium hydroxide (NaOH) solution for examination of male genitalia, photographs of which were taken under a Keyence VHX-5000 digital microscope. Terminology of adult and genitalia follows
Molecular phylogenetic analysis. We selected 19 samples representing the species of Olepa as the ingroup and, for the outgroup, we used Trischalis aureoplagiata and Cyclosiella spiralis (two species of the tribe Lithosiini of the subfamily Arctiinae). We sequenced four specimens from China and the remaining sequences were obtained from GenBank and Bold Systems. DNA was extracted from two or three legs of dried adult specimens using a TIANamp Genomic DNA Kit (Tiangen, Guangzhou, China) following the manufacturer’s instructions. We amplified a single mitochondrial gene (a 658-bp fragment of COI) using the general primers 1490-2198 (
Voucher information and accession numbers for the specimens in this study.
Taxon | Locality | Date | Voucher Number | Accession Number |
Olepa ricini | China | 24-Ⅸ-2020 | SCAU Ole01 | OK576935 |
Olepa ricini | China | 14-Ⅸ-2021 | SCAU Ole02 | OK576936 |
Olepa ricini | China | 1-Ⅹ-2021 | SCAU Ole03 | OK576937 |
Olepa ricini | China | 13-Ⅶ-2020 | SCAU Ole04 | OK576938 |
Olepa schleini | Israel | 19-Ⅶ-2005 | DNATAX02723 | AM050270* |
Olepa schleini | Israel | 19-Ⅶ-2005 | DNATAX02724 | AM050271* |
Olepa schleini | Israel | 19-Ⅶ-2005 | DNATAX02725 | AM050272* |
Olepa schleini | India | 07-Ⅹ-2017 | ZSI_WRC_L_2029 | DBFWG011-21+ |
Olepa schleini | India | 24-Ⅱ-2019 | MOGSJ 03 | DBEM003-20+ |
Olepa schleini | India | 04-Ⅶ-2019 | ZSI_WRC_L_2028 | DBFWG004-20+ |
Olepa suryamal | India | 07-Ⅹ-2017 | ZSI_WRC_L_2148 | DBFWG006-21+ |
Olepa suryamal | India | 07-Ⅹ-2017 | ZSI_WRC_L_2149 | DBFWG007-21+ |
Olepa suryamal | India | 07-Ⅹ-2017 | ZSI_WRC_L_2150 | DBFWG012-21+ |
Olepa zedesi | India | 23-Ⅷ-2017 | ZSI_WRC_L_2154 | DBFWG009-21+ |
Olepa zedesi | India | 17-Ⅷ-2017 | ZSI_WRC_L_2155 | DBFWG010-21+ |
Olepa ricini | India | N/A | N/A | KY559102* |
Olepa ricini | India | 05-ⅩⅡ-2012 | RO_Olepric-1 | KX371816* |
Olepa toulgoeti | India | 4-Ⅳ-1997 | DNATAX02741 | AM050285* |
Olepa toulgoeti | India | 4-Ⅳ-1997 | DNATAX02742 | AM050286* |
Trischalis aureoplagiata | Australia | 15-Ⅲ-1992 | 10ANIC-00599 | HQ921017* |
Cyclosiella spiralis | Malaysia | 18-Ⅴ-2014 | BIOUG14394-C09 | LEPMY1189+ |
* Indicates that the gene sequence was downloaded from NCBI, + Indicates that the gene sequence was downloaded from Bold Systems.
Bombyx ricini
Alope ricini Moore, 1882: 70
Pericallia ricini Hampson, 1901, 350
Olepa ricini Watson, 1980:133
Olepa (Ricinia) ricini; Singh & Singh, 2013:276
Olepa neumuthi Orhant, 2012:61, synonymised by Singh & Singh, 2013
Olepa schleini Witt et al., 2005:102 (Type locality: Israel, Tel Aviv North), syn. nov.
Male (Fig.
Male genitalia (Fig.
Female: unknown.
China (new record), Thailand, India, Israel.
The Chinese population of Olepa is morphologically similar to O. schleini and O. ricini. These two species were supposed to be distinguished from each other mainly by the differences in the tip of the valva, i.e. tip rounded in the former and more acute in the latter, together with a great genetic difference in COI sequences (
We are grateful to Dr. XL Fan for guiding the experiment and helping to revise the manuscript, Mr. Hou, Mr. Qin and Mr. Zhou for providing specimens and the Cave Biology Laboratory of South China Agricultural University for taking male genitalia photographs.