Biodiversity Data Journal :
Data Paper (Biosciences)
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Corresponding author: Yingchun Xing (xingych@cafs.ac.cn)
Academic editor: Rupert Collins
Received: 30 Jun 2022 | Accepted: 27 Sep 2022 | Published: 30 Sep 2022
© 2022 Rui Xi, Wanru Gao, Xin Wang, Yingchun Xing
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Xi R, Gao W, Wang X, Xing Y (2022) Species diversity of coral reef fishes around the West Island of Sanya City, South China Sea, based on environmental DNA. Biodiversity Data Journal 10: e89685. https://doi.org/10.3897/BDJ.10.e89685
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West Island is the second largest island in the Hainan Province, China and its surrounding sea area has a well-preserved coral reef ecosystem and high species diversity of coral reef fishes. Undoubtedly, coral reef fishes and coral reefs have complex symbiotic relationships and fish species diversity should reflect the healthy status of coral reef ecosystems. Environmental DNA (eDNA) is a useful and sensitive tool to detect fish species and causes less environmental damage than traditional fish survey methods. This paper investigated coral reef fish species of West Island, Hainan Province, China, based on eDNA and provided scientific data for understanding and protection of the coral reef ecosystem of the South China Sea.
The sea area surrounding West Island is the most important component of the coral reef ecosystem in the northern part of the South China Sea, which is also an essential part of the largest National Coral Reef Reserve in China. However, complete data of fish species distributed in this region have been a long-term gap. This study provides information on 41 fish species belonging to 28 genera, 16 families and three orders in this sea area and is the first complete record of coral reef fishes surrounding the West Island. In addition, the information of Molecular Operational Taxonomic Units (MOTUs) for taxon identification were also provided and it could contribute to building specific eDNA taxonomy database of coral reef fishes of the South China Sea. The study includes three datasets, with aspects of fish taxon-occurrences, MOTUs sequences and information of environmental indicators surrounding West Island, Hainan Province, China. The “fish taxon occurrences” dataset presents records involving taxonomic, distribution, habitat condition, latitude and longitude of 41 coral reef fish species detected, based on eDNA, the “MOTUs sequences” dataset provides MOTUs sequences and their abundance of 31 species detected and the “information of environmental indicators” dataset presents records of transparency, temperature, water pressure, dissolved oxygen, electrical conductivity, hydrogen and redox potential measured from five sampling localities.
coral reef ecosystem, occurrence of fish taxon, distribution, habitat condition, sequences of fish MOTUs, South China Sea
The coral reef is an important tropical marine ecosystem and it provides abundant habitats for fish communities (
Environmental DNA (eDNA) is derived from metabolic waste, damaged tissue or sloughed skin cells of the organisms released into the environment and is used for detecting specific fish species through amplifying a target molecular marker or detecting multiple species through DNA metabarcoding (
The surrounding sea area of West Island is located in the nearshore of Sanya City, Hainan Province, China.
The surface water samples (under water 8 m) were collected using 3 l water sampler at five localities in the sea area surrounding West Island, Hainan Province, China in June 2021 (Fig.
The eDNA was extracted using the DNeasy Blood and Tissue Kit (QIAGEN, Germany) and the following modifications were made compared to the spin column protocol: a) add 720 µl ATL Buffer and 80 µl Proteinase K into centrifuge tubes containing filtration membranes and incubated 3-4 hours at 56°C; b) add 800 µl AL Buffer and 800 µl 96% ethanol after incubation; c) elution was done in 2 × 50 μl TE Buffer for a final volume of 100 μl. For each round of extraction, an extraction blank sample was made for monitoring cross-contamination, with smaller volumes being added: 180 μl ATL Buffer, 20 μl Proteinase K, 200 μl AL Buffer and 200 μl 96% ethanol. The laboratory tables, aseptic operation tables and experimental equipment were regularly cleaned with 5% bleach and then 75% ethanol before DNA extraction. The extracted DNA samples were stored at -20°C.
PCR amplification was performed using “Tele02” primers developed by
Sample ID |
OligoTags |
XD-1 |
GAACTA |
XD-2 |
AGTGTT |
XD-3 |
AAGACA |
XD-4 |
TACTTC |
XD-5 |
GTCTTA |
The raw sequences obtained from the Illumina Novaseq platform were initially processed using QIIME 2 software (
We surveyed five localities in the surrounding sea area of West Island (Fig.
18.230 and 18.239 Latitude; 109.347 and 109.369 Longitude.
In total, three orders, 16 families, 28 genera and 41 coral reef fish species were detected by using eDNA in the surrounding sea area of West Island.
Rank | Scientific Name |
---|---|
kingdom | Animalia |
phylum | Chordata |
class | Osteichthyes |
order | Perciformes |
order | Clupeiformes |
order | Atheriniformes |
family | Carangidae |
family | Lutjanidae |
family | Pomacentridae |
family | Blenniidae |
family | Tripterygiidae |
family | Gerreidae |
family | Gobiidae |
family | Apogonidae |
family | Labridae |
family | Siganidae |
family | Sphyraenidae |
family | Rachycentridae |
family | Leiognathidae |
family | Clupeidae |
family | Engraulidae |
family | Atherinidae |
genus | Trachurus |
genus | Trachinotus |
genus | Selar |
genus | Megalaspis |
genus | Alepes |
genus | Decapterus |
genus | Lutjanus |
genus | Abudefduf |
genus | Neopomacentrus |
genus | Blenniella |
genus | Enneapterygius |
genus | Gerres |
genus | Asterropteryx |
genus | Ostorhinchus |
genus | Stethojulis |
genus | Siganus |
genus | Sphyraena |
genus | Rachycentron |
genus | Hilsa |
genus | Herklotsichthys |
genus | Sardinella |
genus | Engraulis |
genus | Encrasicholina |
genus | Atherinomorus |
species | Trachurus japonicus (Temminck & Schlegel, 1844) |
species | Trachinotus ovatus (Linnaeus,1758) |
species | Trachinotus blochii (Lacepède, 1801) |
species | Selar crumenophthalmus (Bloch,1793) |
species | Megalaspis cordyla (Linnaeus,1758) |
species | Alepes kleinii (Bloch,1793) |
species | Alepes djedaba (Forsskål, 1775) |
species | Alepes melanoptera (Swainson,1839) |
species | Decapterus maruadsi (Temminck & Schlegel,1843) |
species | Lutjanus argentimaculatus (Forsskål, 1775) |
species | Lutjanus fulviflamma (Forsskål, 1775) |
species | Lutjanus sebae (Cuvier,1816) |
species | Abudefduf vaigiensis (Quoy & Gaimard,1825) |
species | Abudefduf sexfasciatus (Lacepède, 1801) |
species | Neopomacentrus azysron (Bleeker,1877) |
species | Blenniella bilitonensis (Bleeker,1858) |
species | Enneapterygius philippinus (Peters,1868) |
species | Gerres filamentosus Cuvier, 1829 |
species | Asterropteryx semipunctata Rüppell, 1830 |
species | Ostorhinchus aureus (Lacepède, 1802) |
species | Stethojulis terina Jordan & Snyder 1902 |
species | Siganus fuscescens (Houttuyn,1782) |
species | Sphyraena jello Cuvier 1829 |
species | Rachycentron canadum (Linnaeus,1766) |
species | Hilsa kelee (Cuvier,1829) |
species | Herklotsichthys quadrimaculatus (Rüppell, 1837) |
species | Sardinella gibbosa (Bleeker, 1849) |
species | Engraulis japonicus Temminck & Schlegel, 1846 |
species | Encrasicholina punctifer Fowler 1938 |
species | Atherinomorus lacunosus (Forster,1801) |
species | Atherinomorus pinguis (Lacepède, 1803) |
species | Lutjanus sp. |
species | Abudefduf sp. |
species | Siganus sp. |
species | Sardinella sp.1 |
species | Sardinella sp.2 |
species | Encrasicholina sp. |
species | Blenniidae gen. sp. |
species | Leiognathidae gen. sp. |
species | Engraulidae gen. sp. |
species | Atherinidae gen. sp. |
2021-6-24
The dataset presents the results of species composition of 31 coral reef fish detected in five sampling localities in the sea area surrounding West Island using eDNA techniques. The important information including taxonomic, geographic location of the occurrence and habitat condition were provided for 10 species (
Column label | Column description |
---|---|
occurrenceID | Unique occurrence identifier. |
basisOfRecord | The specific nature of the data record. |
eventDate | Date of sampling event. |
scientificName | The full scientific name. |
verbatimIdentification | A string representing the taxonomic identification as it appeared in the original record. |
kingdom | The full scientific name of the kingdom in which the taxon is classified. |
Phylum | The full scientific name of the phylum or division in which the taxon is classified. |
Class | The full scientific name of the class in which the taxon is classified. |
Order | The full scientific name of the order in which the taxon is classified. |
Family | The full scientific name of the family in which the taxon is classified. |
Genus | The full scientific name of the genus in which the taxon is classified. |
taxonRank | The taxonomic rank of the most specific name in the scientificName as it appears in the original record. |
locality | The specific description of the county from where specimens are collected. |
county | The full, unabbreviated name of the next smaller administrative region than stateProvince (county, shire, department etc.) in which the Location occurs. |
stateProvince | The name of the next smallest administrative region than country (state, province, canton, department, region etc.) in which the Location occurs. |
Country | The full, unabbreviated name of the country where the organism was collected. |
waterBody | The name of the water body in which the Location occurs. |
habitat | A category or description of the habitat in which the Event occurred. |
locationID | A spatial region or named place. The locationID refers to serial number of each sampling site in this study. |
decimalLatitude | The geographic latitude (in decimal degrees, using the spatial reference system given in geodeticDatum) of the geographic centre of a Location. |
decimalLongitude | The geographic longitude (in decimal degrees, using the spatial reference system given in geodeticDatum) of the geographic centre of a Location. |
geodeticDatum | The geographic information system (GIS) upon which the geographic coordinates given in decimalLatitude, decimalLongitude and meterElevation are based. |
The dataset presents the nucleotides sequence and abundance of sequences of each fish species MOTU, through high-throughput sequencing of eDNA samples collected in the sea area surrounding West Island. Considering another 10 MOTUs could not be identified to definite species, and there is no single MOTU sequence, these MOTUs have not been provided in this dataset (Suppl. material
Column label | Column description |
---|---|
scientificName | The full scientific name. |
associatedSequences | A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the Occurrence. The associatedSequences refers to MOTU sequences of each scientificName. |
organismQuantity | A number or enumeration value for the quantity of organisms. |
organismQuantityType | The type of quantification system used for the quantity of organisms. |
dateIdentified | The date on which the subject was determined as representing the Taxon. |
identificationReferences | A list (concatenated and separated) of references (publication, global unique identifier, URI) used in the Identification. |
identificationRemarks | Comments or notes about the Identification. |
The dataset presents records of seven regular marine environmental indicators, including transparency, temperature, water pressure, dissolved oxygen, electrical conductivity, hydrogen and redox potential. These records were detected at the same time when eDNA was collected (Suppl. material
Column label | Column description |
---|---|
eventDate | Date of sampling event. |
locationID | A spatial region or named place. The locationID refers to serial number of each sampling site in this study. |
decimalLatitude | The geographic latitude (in decimal degrees, using the spatial reference system given in geodeticDatum) of the geographic centre of a Location. |
decimalLongitude | The geographic longitude (in decimal degrees, using the spatial reference system given in geodeticDatum) of the geographic centre of a Location. |
measurementID | An identifier for the MeasurementOrFact (information pertaining to measurements, facts, characteristics or assertions). May be a global unique identifier or an identifier specific to the dataset. The measurementID refers to serial number of each environmental sample in this study. |
measurementType | The nature of the measurement, fact, characteristic or assertion. Recommended best practice is to use a controlled vocabulary. |
measurementValue | The value of the measurement, fact, characteristic or assertion. |
measurementUnit | The units associated with the measurementValue. |
measurementDeterminedDate | The date on which the MeasurementOrFact was made. |
We analysed the fish composition, based on eDNA results (Fig.
Herein, 31 coral reef fish species have been identified according to sequences of MOTUs amongst all species obtained. In contrast, another 10 MOTUs could not be identified to definite species, so we only represented them as indeterminate species of known genus or family, using “sp.” as the species name of them. The reason for this dilemma mainly is incomplete reference DNA database of Chinese marine fishes, especially tropical fishes. Although NCBI was still considered as a widely accepted database for alignment of DNA sequences and species annotation, data of tropical fishes are lacking (
This study is funded by the Hainan Provincial Joint Project of Sanya-Yazhou Bay Science and Technology City Grant (No.320LH069), the Key research and development programme of Hainan Province (No. ZDYF2022SHFZ027) and Central Public-interest Scientific Institution Basal Research Fund, CAFS (NO. 2020TD11) to YX. We appreciated help from Mr. Youming Chen and Mr. Bixing Li working in the Hainan Blue Granary Technology Co., Ltd during water sampling.
Rui Xi prepared datasets, analysed data and drafted the manuscript. Yingchun Xing, Wanru Gao and Xin Wang performed the fieldwork. Wanru Gao performed eDNA laboratory work. All co-authors gave their comments on this manuscript.
This dataset provides taxonomic, distribution, habitat condition, latitude and longitude of each species of 41 coral reef-fish species detected, based on eDNA from the surrounding sea area of West Island.
This dataset provides MOTUs sequences and abundances of sequences of 31 coral-reef fish species detected by eDNA in the surrounding sea area of West Island.
This dataset provides the measurement values of environmental indicators of five sampling sites surrounding West Island.