Biodiversity Data Journal :
OMIC Data Paper
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Corresponding author: Gaëtan Rongier (rongiergaetan15@gmail.com), Jerome Orivel (jerome.orivel@cnrs.fr)
Academic editor: Brian Lee Fisher
Received: 12 Aug 2022 | Accepted: 30 Nov 2022 | Published: 07 Feb 2023
© 2023 Gaëtan Rongier, Audrey Sagne, Sandrine Etienne, Frederic Petitclerc, Gaelle Jaouen, Jerome Murienne, Jerome Orivel
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Rongier G, Sagne A, Etienne S, Petitclerc F, Jaouen G, Murienne J, Orivel J (2023) Ants of French Guiana: 16S rRNA sequence dataset. Biodiversity Data Journal 11: e91577. https://doi.org/10.3897/BDJ.11.e91577
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This dataset represents a reference library of DNA sequences for ants from French Guiana. A total of 3931 new sequences from the 16S rRNA gene has been generated. The reference library covers 344 species distributed in 57 genera. Overall, 3920 sequences have been assigned at the species level and 11 at the genus level. All these sequences were submitted to DDBJ/EMBL/GenBank databases in the Bioproject: PRJNA779056: 16S French Guiana Ants (Hymenoptera: Formicidae), sequence identifier KFFS00000000.
DNA sequencing, 16S rRNA, molecular identification, Formicidae, NGS, Neotropics
The current biodiversity crisis calls for efforts to reach more rapid biodiversity characterisation. Indeed, our global knowledge of biodiversity is still largely unknown, with ca. 80% of species to be described and more than 20 years, on average, for the description of a new species following its discovery (
With more than 16,000 described species to date (
French Guiana, the largest French overseas territory, is located in the Guiana shield on the north-eastern coast of South America. Covered with primary forest on more than 90% of its surface, it is part of the largest block of tropical forest worldwide, hosting a large diversity of species. As an example, the recent checklist of ants from French Guiana highlighted the presence of 659 valid species and subspecies from 84 genera and 12 subfamilies, representing ca. 10% of the ant diversity known in the Neotropical realm (
Ants were sampled from 2013 onwards in a diversity of sites covering most of the major forest habitats represented in French Guina (
Sampling was performed following the Ants of Leaf Litter Protocol (
Specimens were preserved in 95% ethanol and then sorted to morphospecies in the lab. One individual of each morphospecies was then mounted for morphological identification to species using taxonomic resources available in the literature and the expertise of taxonomy specialists. Voucher specimens were deposited in the Laboratorio de Mirmecologia, Cocoa Research Centre CEPEC/CEPLAC (Itabuna, BA, Brazil) and at EcoFoG in Kourou.
Although the mitochondrial gene encoding the cytochrome c oxidase subunit 1 (COI) has been accepted has the consensus marker (
DNA extraction was performed from single leg or whole specimen for the smallest, with at least three specimens per species. Each extract was amplified by PCR with the 16S rRNA primer Ins16S_1 (
Sequence data (Suppl. material
The quality of the sequences (Suppl. material
Notes: 2013-present
This dataset (Suppl. material
This Targeted Locus Study project has been deposited at DDBJ/EMBL/GenBank under the accession number KFFS00000000. The version described in this paper is the first version, KFFS01000000.
Financial support for this study was provided by Investissement d'Avenir grants of the Agence Nationale de la Recherche (CEBA: ANR- 10-LABX-25-01; DRIIHM: ANR-11-LABX-0010; TULIP: ANR-10-LABX-41), by the Programme Convergence 2007-2013, Région Guyane from the European community (BREGA, 757/2014/SGAR/DE/BSF) and by the PO-FEDER 2014-2020, Région Guyane (BiNG, GY0007194 and BUG, GY0024253). We would like to thank Sébastien Cally and Anna Grandchamp who participated with specimen sequencing and data analysis. We thank the national park and natural reserve managers for allowing our research programme in the protected areas. Specimens from Itoupé and Mitaraka were collected in the core area of the Parc Amazonien de Guyane. The Itoupé expedition was organised and conducted in collaboration with the Parc Amazonien de Guyane. The Mitaraka expedition was part of the “Our Planet Reviewed” French Guiana-2015 initiative organised by the Muséum National d’Histoire Naturelle (Paris) and the NGO Pro-Natura International and funded by the European Regional Development Fund (ERDF), the Conseil Régional de Guyane, the Conseil Général de Guyane, the Direction de l'Environnement, de l'Aménagement et du Logement (DEAL) and by the Ministère de l'Éducation nationale, de l'Enseignement Supérieur et de la Recherche. Specimens from the Trinité area were collected in the Réserve Naturelle Nationale de La Trinité managed by the Office National des Forêts. The expedition was funded by the Réserve Naturelle Nationale de La Trinité and the DEAL Guyane. Data have been collected from access to genetic resources in French Guiana, that has come through a declarative process with non-commercial uses at the competent administrative authority, in accordance with article L.421-7 of the environmental code (Authorization number TREL1820249A/51; APA-973-1 and ABSCH-IRCC-FR-253854-1).
The authors declare no conflicts of interests.